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Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258

Klebsiella pneumoniae clonal group (CG) 258, comprising sequence types (STs) 258, 11, and closely related variants, is associated with dissemination of the K. pneumoniae carbapenemase (KPC). Hospital outbreaks of KPC CG258 infections have been observed globally and are very difficult to treat. As a...

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Autores principales: Wyres, Kelly L., Gorrie, Claire, Edwards, David J., Wertheim, Heiman F.L., Hsu, Li Yang, Van Kinh, Nguyen, Zadoks, Ruth, Baker, Stephen, Holt, Kathryn E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453057/
https://www.ncbi.nlm.nih.gov/pubmed/25861820
http://dx.doi.org/10.1093/gbe/evv062
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author Wyres, Kelly L.
Gorrie, Claire
Edwards, David J.
Wertheim, Heiman F.L.
Hsu, Li Yang
Van Kinh, Nguyen
Zadoks, Ruth
Baker, Stephen
Holt, Kathryn E.
author_facet Wyres, Kelly L.
Gorrie, Claire
Edwards, David J.
Wertheim, Heiman F.L.
Hsu, Li Yang
Van Kinh, Nguyen
Zadoks, Ruth
Baker, Stephen
Holt, Kathryn E.
author_sort Wyres, Kelly L.
collection PubMed
description Klebsiella pneumoniae clonal group (CG) 258, comprising sequence types (STs) 258, 11, and closely related variants, is associated with dissemination of the K. pneumoniae carbapenemase (KPC). Hospital outbreaks of KPC CG258 infections have been observed globally and are very difficult to treat. As a consequence, there is renewed interest in alternative infection control measures such as vaccines and phage or depolymerase treatments targeting the K. pneumoniae polysaccharide capsule. To date, 78 immunologically distinct capsule variants have been described in K. pneumoniae. Previous investigations of ST258 and a small number of closely related strains suggested that capsular variation was limited within this clone; only two distinct ST258 capsule polysaccharide synthesis (cps) loci have been identified, both acquired through large-scale recombination events (>50 kb). In contrast to previous studies, we report a comparative genomic analysis of the broader K. pneumoniae CG258 (n = 39). We identified 11 different cps loci within CG258, indicating that capsular switching is actually common within the complex. We observed several insertion sequences (IS) within the cps loci, and show further intraclone diversification of two cps loci through IS activity. Our data also indicate that several large-scale recombination events have shaped the genomes of CG258, and that definition of the complex should be broadened to include ST395 (also reported to harbor KPC). As only the second report of extensive intraclonal cps variation among Gram-negative bacterial species, our findings alter our understanding of the evolution of these organisms and have key implications for the design of control measures targeting K. pneumoniae capsules.
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spelling pubmed-44530572015-06-10 Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258 Wyres, Kelly L. Gorrie, Claire Edwards, David J. Wertheim, Heiman F.L. Hsu, Li Yang Van Kinh, Nguyen Zadoks, Ruth Baker, Stephen Holt, Kathryn E. Genome Biol Evol Research Article Klebsiella pneumoniae clonal group (CG) 258, comprising sequence types (STs) 258, 11, and closely related variants, is associated with dissemination of the K. pneumoniae carbapenemase (KPC). Hospital outbreaks of KPC CG258 infections have been observed globally and are very difficult to treat. As a consequence, there is renewed interest in alternative infection control measures such as vaccines and phage or depolymerase treatments targeting the K. pneumoniae polysaccharide capsule. To date, 78 immunologically distinct capsule variants have been described in K. pneumoniae. Previous investigations of ST258 and a small number of closely related strains suggested that capsular variation was limited within this clone; only two distinct ST258 capsule polysaccharide synthesis (cps) loci have been identified, both acquired through large-scale recombination events (>50 kb). In contrast to previous studies, we report a comparative genomic analysis of the broader K. pneumoniae CG258 (n = 39). We identified 11 different cps loci within CG258, indicating that capsular switching is actually common within the complex. We observed several insertion sequences (IS) within the cps loci, and show further intraclone diversification of two cps loci through IS activity. Our data also indicate that several large-scale recombination events have shaped the genomes of CG258, and that definition of the complex should be broadened to include ST395 (also reported to harbor KPC). As only the second report of extensive intraclonal cps variation among Gram-negative bacterial species, our findings alter our understanding of the evolution of these organisms and have key implications for the design of control measures targeting K. pneumoniae capsules. Oxford University Press 2015-04-10 /pmc/articles/PMC4453057/ /pubmed/25861820 http://dx.doi.org/10.1093/gbe/evv062 Text en © The Author(s) 2015. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Wyres, Kelly L.
Gorrie, Claire
Edwards, David J.
Wertheim, Heiman F.L.
Hsu, Li Yang
Van Kinh, Nguyen
Zadoks, Ruth
Baker, Stephen
Holt, Kathryn E.
Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258
title Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258
title_full Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258
title_fullStr Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258
title_full_unstemmed Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258
title_short Extensive Capsule Locus Variation and Large-Scale Genomic Recombination within the Klebsiella pneumoniae Clonal Group 258
title_sort extensive capsule locus variation and large-scale genomic recombination within the klebsiella pneumoniae clonal group 258
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453057/
https://www.ncbi.nlm.nih.gov/pubmed/25861820
http://dx.doi.org/10.1093/gbe/evv062
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