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miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop
BACKGROUND: The effective mechanisms of microRNAs (miRNAs) functions as oncogenes or tumour suppressors in human hepatocellular carcinoma (HCC) are still obscure. Here, we investigated the function and expression of miR-1228 in HCC. METHODS: The role of miR-1228 in HCC was determined by colony forma...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453453/ https://www.ncbi.nlm.nih.gov/pubmed/25422913 http://dx.doi.org/10.1038/bjc.2014.593 |
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author | Zhang, Y Dai, J Deng, H Wan, H Liu, M Wang, J Li, S Li, X Tang, H |
author_facet | Zhang, Y Dai, J Deng, H Wan, H Liu, M Wang, J Li, S Li, X Tang, H |
author_sort | Zhang, Y |
collection | PubMed |
description | BACKGROUND: The effective mechanisms of microRNAs (miRNAs) functions as oncogenes or tumour suppressors in human hepatocellular carcinoma (HCC) are still obscure. Here, we investigated the function and expression of miR-1228 in HCC. METHODS: The role of miR-1228 in HCC was determined by colony formation, transwell, and nude mice xenograft experiments. miR-1228 target gene were identified by EGFP reporter assays, real-time PCR, and western blot analysis. Dual-luciferase reporter assay and real-time PCR analysis are used to examine the regulation of p53. RESULTS: miR-1228 promoted proliferation and metastasis, and facilitated the transition of cell cycle in hepatoma cells. miR-1228 downregulated p53 expression by binding to its 3′UTR. The ectopic expression of p53 abrogated the phenotypes induced by miR-1228. An inverse correlation existed between miR-1228 and p53 expression in hepatoma tissues compared with the adjacent tissues and three hepatoma cell lines. Moreover, we found that p53 suppressed the expression and promoter activity of miR-1228. CONCLUSIONS: miR-1228 functions as an oncogene by promoting cell cycle progression and cell mobility and negatively regulates the expression of p53. p53 downregulation in turn leads to an increase in miR-1228 expression, thereby forming a positive feedback loop that contributes to cancerogenesis in HCC. |
format | Online Article Text |
id | pubmed-4453453 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44534532016-01-20 miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop Zhang, Y Dai, J Deng, H Wan, H Liu, M Wang, J Li, S Li, X Tang, H Br J Cancer Molecular Diagnostics BACKGROUND: The effective mechanisms of microRNAs (miRNAs) functions as oncogenes or tumour suppressors in human hepatocellular carcinoma (HCC) are still obscure. Here, we investigated the function and expression of miR-1228 in HCC. METHODS: The role of miR-1228 in HCC was determined by colony formation, transwell, and nude mice xenograft experiments. miR-1228 target gene were identified by EGFP reporter assays, real-time PCR, and western blot analysis. Dual-luciferase reporter assay and real-time PCR analysis are used to examine the regulation of p53. RESULTS: miR-1228 promoted proliferation and metastasis, and facilitated the transition of cell cycle in hepatoma cells. miR-1228 downregulated p53 expression by binding to its 3′UTR. The ectopic expression of p53 abrogated the phenotypes induced by miR-1228. An inverse correlation existed between miR-1228 and p53 expression in hepatoma tissues compared with the adjacent tissues and three hepatoma cell lines. Moreover, we found that p53 suppressed the expression and promoter activity of miR-1228. CONCLUSIONS: miR-1228 functions as an oncogene by promoting cell cycle progression and cell mobility and negatively regulates the expression of p53. p53 downregulation in turn leads to an increase in miR-1228 expression, thereby forming a positive feedback loop that contributes to cancerogenesis in HCC. Nature Publishing Group 2015-01-20 2014-11-25 /pmc/articles/PMC4453453/ /pubmed/25422913 http://dx.doi.org/10.1038/bjc.2014.593 Text en Copyright © 2015 Cancer Research UK http://creativecommons.org/licenses/by-nc-sa/3.0/ From twelve months after its original publication, this work is licensed under the Creative Commons Attribution-NonCommercial-Share Alike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ |
spellingShingle | Molecular Diagnostics Zhang, Y Dai, J Deng, H Wan, H Liu, M Wang, J Li, S Li, X Tang, H miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop |
title | miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop |
title_full | miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop |
title_fullStr | miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop |
title_full_unstemmed | miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop |
title_short | miR-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop |
title_sort | mir-1228 promotes the proliferation and metastasis of hepatoma cells through a p53 forward feedback loop |
topic | Molecular Diagnostics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453453/ https://www.ncbi.nlm.nih.gov/pubmed/25422913 http://dx.doi.org/10.1038/bjc.2014.593 |
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