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A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene

BACKGROUND: Surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. In this study beta tubulin gene (BT2), was used as the first time in a PCR-RFLP format to clarify the distribution of dermatophytosis agents in s...

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Autores principales: ABASTABAR, Mahdi, REZAEI-MATEHKOLAEI, Ali, SHIDFAR, Mohammad Reza, KORDBACHEH, Parivash, MOHAMMADI, Rasoul, SHOKOOHI, Tahereh, HEDAYATI, Mohammad Taghi, JALALIZAND, Nilufar, MIRHENDI, Hossein
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Tehran University of Medical Sciences 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453885/
https://www.ncbi.nlm.nih.gov/pubmed/26060667
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author ABASTABAR, Mahdi
REZAEI-MATEHKOLAEI, Ali
SHIDFAR, Mohammad Reza
KORDBACHEH, Parivash
MOHAMMADI, Rasoul
SHOKOOHI, Tahereh
HEDAYATI, Mohammad Taghi
JALALIZAND, Nilufar
MIRHENDI, Hossein
author_facet ABASTABAR, Mahdi
REZAEI-MATEHKOLAEI, Ali
SHIDFAR, Mohammad Reza
KORDBACHEH, Parivash
MOHAMMADI, Rasoul
SHOKOOHI, Tahereh
HEDAYATI, Mohammad Taghi
JALALIZAND, Nilufar
MIRHENDI, Hossein
author_sort ABASTABAR, Mahdi
collection PubMed
description BACKGROUND: Surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. In this study beta tubulin gene (BT2), was used as the first time in a PCR-RFLP format to clarify the distribution of dermatophytosis agents in some parts of Iran. METHODS: A total of 603 clinical isolates was obtained from 500 patients in Tehran, Isfahan, Mazandaran and Guilan provinces. The isolates were identified using macro/micro-morphological criteria and electrophoretic patterns of PCR amplicons of BT2after digestion with each of the restriction enzymes FatI, HpyCH4V, MwoI and Alw21I. RESULTS: Among the patients, 59.2% were male and 40.8% female. The most prevalent clinical form was tinea pedis (42.4%), followed by tinea cruris (24.2%), tinea unguium (12.3%), tinea corporis (10.8%), tinea faciei (4%), tinea manuum (3.14%), tinea capitis (3%) and tinea barbae (0.16%), respectively. Trichophyton interdigitale ranked the first, followed by T. rubrum, Epidermophyton floccosum, Microsporum canis, T. tonsurans, T. erinacei and T. violaceum (each 0.49%) and the less frequent species were T. schoenleinii, M. gypseum and T.anamorph of Arthroderma benhamiae (each 0.16%). A case of scalp infection by E. floccosum was an exceptional event in the study. No case of T. verrucosum was found. CONCLUSION: Trichophyton species and E. floccosum are yet the predominant agents of infection in Iran, while Microsporum species are decreasing. T. interdigitale and Tinea pedis remain as the most causal agent and clinical form of dermatophytosis, respectively. It seems that BT2 can be a useful genetic marker for epidemiological survey of common pathogenic dermatophytes.
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spelling pubmed-44538852015-06-09 A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene ABASTABAR, Mahdi REZAEI-MATEHKOLAEI, Ali SHIDFAR, Mohammad Reza KORDBACHEH, Parivash MOHAMMADI, Rasoul SHOKOOHI, Tahereh HEDAYATI, Mohammad Taghi JALALIZAND, Nilufar MIRHENDI, Hossein Iran J Public Health Original Article BACKGROUND: Surveillance of dermatophytosis is essential to determine the likely changes in etiological trends and distribution profile of this infection. In this study beta tubulin gene (BT2), was used as the first time in a PCR-RFLP format to clarify the distribution of dermatophytosis agents in some parts of Iran. METHODS: A total of 603 clinical isolates was obtained from 500 patients in Tehran, Isfahan, Mazandaran and Guilan provinces. The isolates were identified using macro/micro-morphological criteria and electrophoretic patterns of PCR amplicons of BT2after digestion with each of the restriction enzymes FatI, HpyCH4V, MwoI and Alw21I. RESULTS: Among the patients, 59.2% were male and 40.8% female. The most prevalent clinical form was tinea pedis (42.4%), followed by tinea cruris (24.2%), tinea unguium (12.3%), tinea corporis (10.8%), tinea faciei (4%), tinea manuum (3.14%), tinea capitis (3%) and tinea barbae (0.16%), respectively. Trichophyton interdigitale ranked the first, followed by T. rubrum, Epidermophyton floccosum, Microsporum canis, T. tonsurans, T. erinacei and T. violaceum (each 0.49%) and the less frequent species were T. schoenleinii, M. gypseum and T.anamorph of Arthroderma benhamiae (each 0.16%). A case of scalp infection by E. floccosum was an exceptional event in the study. No case of T. verrucosum was found. CONCLUSION: Trichophyton species and E. floccosum are yet the predominant agents of infection in Iran, while Microsporum species are decreasing. T. interdigitale and Tinea pedis remain as the most causal agent and clinical form of dermatophytosis, respectively. It seems that BT2 can be a useful genetic marker for epidemiological survey of common pathogenic dermatophytes. Tehran University of Medical Sciences 2013-09 /pmc/articles/PMC4453885/ /pubmed/26060667 Text en Copyright © Iranian Public Health Association & Tehran University of Medical Sciences This work is licensed under a Creative Commons Attribution-NonCommercial 3.0 Unported License which allows users to read, copy, distribute and make derivative works for non-commercial purposes from the material, as long as the author of the original work is cited properly.
spellingShingle Original Article
ABASTABAR, Mahdi
REZAEI-MATEHKOLAEI, Ali
SHIDFAR, Mohammad Reza
KORDBACHEH, Parivash
MOHAMMADI, Rasoul
SHOKOOHI, Tahereh
HEDAYATI, Mohammad Taghi
JALALIZAND, Nilufar
MIRHENDI, Hossein
A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene
title A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene
title_full A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene
title_fullStr A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene
title_full_unstemmed A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene
title_short A Molecular Epidemiological Survey of Clinically Important Dermatophytes in Iran Based on Specific RFLP Profiles of Beta-tubulin Gene
title_sort molecular epidemiological survey of clinically important dermatophytes in iran based on specific rflp profiles of beta-tubulin gene
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4453885/
https://www.ncbi.nlm.nih.gov/pubmed/26060667
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