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Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1
Nowadays, the knowledge in DNA methylation-mediated gene regulation has shed light on the understanding of virus-host interplay in the context of genome alteration. It has also been shown that HIV is able to change the DNA methylation pattern by DNA methyltransferases and such changes can be correla...
Autores principales: | , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454074/ https://www.ncbi.nlm.nih.gov/pubmed/26039376 http://dx.doi.org/10.1038/srep10806 |
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author | Zhang, Yinfeng Li, Sai-Kam Yi Yang, Kevin Liu, Minghua Lee, Nelson Tang, Xian Wang, Hui Liu, Li Chen, Zhiwei Zhang, Chiyu Wang, Jianhua Kwok-Wing Tsui, Stephen |
author_facet | Zhang, Yinfeng Li, Sai-Kam Yi Yang, Kevin Liu, Minghua Lee, Nelson Tang, Xian Wang, Hui Liu, Li Chen, Zhiwei Zhang, Chiyu Wang, Jianhua Kwok-Wing Tsui, Stephen |
author_sort | Zhang, Yinfeng |
collection | PubMed |
description | Nowadays, the knowledge in DNA methylation-mediated gene regulation has shed light on the understanding of virus-host interplay in the context of genome alteration. It has also been shown that HIV is able to change the DNA methylation pattern by DNA methyltransferases and such changes can be correlated with the progression of AIDS. In this study, we have investigated the relationship between genome-wide DNA methylation pattern and HIV infection using the methylated DNA immunoprecipitation - microarray method. A pair of monozygotic twins was recruited: one of the twins was infected with HIV while the other was not. Based on data from the microarray experiment, 4679 differentially methylated regions in the HIV positive subject with the significant peak values were identified. Selected genes were then validated in human T lymphocyte CEM*174 cell line and HIV/AIDS patients by comparing with normal subjects. We found that IGFBP6 and SATB2 were significantly down-regulated in HIV-infected CEM*174 cells and 3 different cohorts of HIV/AIDS patients while their promoters were predominantly hyper-methylated compared with normal controls. This study also provides a resource for the identification of HIV-induced methylation and contributes to better understanding of the development of HIV/AIDS. |
format | Online Article Text |
id | pubmed-4454074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Nature Publishing Group |
record_format | MEDLINE/PubMed |
spelling | pubmed-44540742015-06-10 Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1 Zhang, Yinfeng Li, Sai-Kam Yi Yang, Kevin Liu, Minghua Lee, Nelson Tang, Xian Wang, Hui Liu, Li Chen, Zhiwei Zhang, Chiyu Wang, Jianhua Kwok-Wing Tsui, Stephen Sci Rep Article Nowadays, the knowledge in DNA methylation-mediated gene regulation has shed light on the understanding of virus-host interplay in the context of genome alteration. It has also been shown that HIV is able to change the DNA methylation pattern by DNA methyltransferases and such changes can be correlated with the progression of AIDS. In this study, we have investigated the relationship between genome-wide DNA methylation pattern and HIV infection using the methylated DNA immunoprecipitation - microarray method. A pair of monozygotic twins was recruited: one of the twins was infected with HIV while the other was not. Based on data from the microarray experiment, 4679 differentially methylated regions in the HIV positive subject with the significant peak values were identified. Selected genes were then validated in human T lymphocyte CEM*174 cell line and HIV/AIDS patients by comparing with normal subjects. We found that IGFBP6 and SATB2 were significantly down-regulated in HIV-infected CEM*174 cells and 3 different cohorts of HIV/AIDS patients while their promoters were predominantly hyper-methylated compared with normal controls. This study also provides a resource for the identification of HIV-induced methylation and contributes to better understanding of the development of HIV/AIDS. Nature Publishing Group 2015-06-03 /pmc/articles/PMC4454074/ /pubmed/26039376 http://dx.doi.org/10.1038/srep10806 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ |
spellingShingle | Article Zhang, Yinfeng Li, Sai-Kam Yi Yang, Kevin Liu, Minghua Lee, Nelson Tang, Xian Wang, Hui Liu, Li Chen, Zhiwei Zhang, Chiyu Wang, Jianhua Kwok-Wing Tsui, Stephen Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1 |
title | Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1 |
title_full | Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1 |
title_fullStr | Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1 |
title_full_unstemmed | Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1 |
title_short | Whole genome methylation array reveals the down-regulation of IGFBP6 and SATB2 by HIV-1 |
title_sort | whole genome methylation array reveals the down-regulation of igfbp6 and satb2 by hiv-1 |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454074/ https://www.ncbi.nlm.nih.gov/pubmed/26039376 http://dx.doi.org/10.1038/srep10806 |
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