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CRISPR/Cas9-mediated reporter knock-in in mouse haploid embryonic stem cells

Mouse parthenogenetic haploid embryonic stem cells (ESCs) are pluripotent cells generated from chemically activated oocytes. Haploid ESCs provide an opportunity to study the effect of genetic alterations because of their hemizygotic characteristics. However, their further application for the selecti...

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Detalles Bibliográficos
Autores principales:	Kimura, Yasuyoshi, Oda, Masaaki, Nakatani, Tsunetoshi, Sekita, Yoichi, Monfort, Asun, Wutz, Anton, Mochizuki, Hideki, Nakano, Toru
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454075/ https://www.ncbi.nlm.nih.gov/pubmed/26039937 http://dx.doi.org/10.1038/srep10710

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