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Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum

The Salmonella enterica serovars Enteritidis, Dublin, and Gallinarum are closely related but differ in virulence and host range. To identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of Enteritidis strain...

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Autores principales: Matthews, T. David, Schmieder, Robert, Silva, Genivaldo G. Z., Busch, Julia, Cassman, Noriko, Dutilh, Bas E., Green, Dawn, Matlock, Brian, Heffernan, Brian, Olsen, Gary J., Farris Hanna, Leigh, Schifferli, Dieter M., Maloy, Stanley, Dinsdale, Elizabeth A., Edwards, Robert A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454671/
https://www.ncbi.nlm.nih.gov/pubmed/26039056
http://dx.doi.org/10.1371/journal.pone.0126883
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author Matthews, T. David
Schmieder, Robert
Silva, Genivaldo G. Z.
Busch, Julia
Cassman, Noriko
Dutilh, Bas E.
Green, Dawn
Matlock, Brian
Heffernan, Brian
Olsen, Gary J.
Farris Hanna, Leigh
Schifferli, Dieter M.
Maloy, Stanley
Dinsdale, Elizabeth A.
Edwards, Robert A.
author_facet Matthews, T. David
Schmieder, Robert
Silva, Genivaldo G. Z.
Busch, Julia
Cassman, Noriko
Dutilh, Bas E.
Green, Dawn
Matlock, Brian
Heffernan, Brian
Olsen, Gary J.
Farris Hanna, Leigh
Schifferli, Dieter M.
Maloy, Stanley
Dinsdale, Elizabeth A.
Edwards, Robert A.
author_sort Matthews, T. David
collection PubMed
description The Salmonella enterica serovars Enteritidis, Dublin, and Gallinarum are closely related but differ in virulence and host range. To identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of Enteritidis strain LK5 and Dublin strain SARB12 and compared these genomes to the publicly available Enteritidis P125109, Dublin CT 02021853 and Dublin SD3246 genome sequences. We also compared the publicly available Gallinarum genome sequences from biotype Gallinarum 287/91 and Pullorum RKS5078. Using bioinformatic approaches, we identified single nucleotide polymorphisms, insertions, deletions, and differences in prophage and pseudogene content between strains belonging to the same serovar. Through our analysis we also identified several prophage cargo genes and pseudogenes that affect virulence and may contribute to a host-specific, systemic lifestyle. These results strongly argue that the Enteritidis, Dublin and Gallinarum serovars of Salmonella enterica evolve by acquiring new genes through horizontal gene transfer, followed by the formation of pseudogenes. The loss of genes necessary for a gastrointestinal lifestyle ultimately leads to a systemic lifestyle and niche exclusion in the host-specific serovars.
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spelling pubmed-44546712015-06-09 Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum Matthews, T. David Schmieder, Robert Silva, Genivaldo G. Z. Busch, Julia Cassman, Noriko Dutilh, Bas E. Green, Dawn Matlock, Brian Heffernan, Brian Olsen, Gary J. Farris Hanna, Leigh Schifferli, Dieter M. Maloy, Stanley Dinsdale, Elizabeth A. Edwards, Robert A. PLoS One Research Article The Salmonella enterica serovars Enteritidis, Dublin, and Gallinarum are closely related but differ in virulence and host range. To identify the genetic elements responsible for these differences and to better understand how these serovars are evolving, we sequenced the genomes of Enteritidis strain LK5 and Dublin strain SARB12 and compared these genomes to the publicly available Enteritidis P125109, Dublin CT 02021853 and Dublin SD3246 genome sequences. We also compared the publicly available Gallinarum genome sequences from biotype Gallinarum 287/91 and Pullorum RKS5078. Using bioinformatic approaches, we identified single nucleotide polymorphisms, insertions, deletions, and differences in prophage and pseudogene content between strains belonging to the same serovar. Through our analysis we also identified several prophage cargo genes and pseudogenes that affect virulence and may contribute to a host-specific, systemic lifestyle. These results strongly argue that the Enteritidis, Dublin and Gallinarum serovars of Salmonella enterica evolve by acquiring new genes through horizontal gene transfer, followed by the formation of pseudogenes. The loss of genes necessary for a gastrointestinal lifestyle ultimately leads to a systemic lifestyle and niche exclusion in the host-specific serovars. Public Library of Science 2015-06-03 /pmc/articles/PMC4454671/ /pubmed/26039056 http://dx.doi.org/10.1371/journal.pone.0126883 Text en © 2015 Matthews et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Matthews, T. David
Schmieder, Robert
Silva, Genivaldo G. Z.
Busch, Julia
Cassman, Noriko
Dutilh, Bas E.
Green, Dawn
Matlock, Brian
Heffernan, Brian
Olsen, Gary J.
Farris Hanna, Leigh
Schifferli, Dieter M.
Maloy, Stanley
Dinsdale, Elizabeth A.
Edwards, Robert A.
Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum
title Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum
title_full Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum
title_fullStr Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum
title_full_unstemmed Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum
title_short Genomic Comparison of the Closely-Related Salmonella enterica Serovars Enteritidis, Dublin and Gallinarum
title_sort genomic comparison of the closely-related salmonella enterica serovars enteritidis, dublin and gallinarum
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454671/
https://www.ncbi.nlm.nih.gov/pubmed/26039056
http://dx.doi.org/10.1371/journal.pone.0126883
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