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Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts

Iron oxide (Fe(3)O(4)) nanoparticles have been used in many biomedical approaches. The toxicity of Fe(3)O(4) nanoparticles on mammalian cells was published recently. Though, little is known about the viability of human cells after treatment with Fe(3)O(4) nanoparticles. Herein, we examined the toxic...

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Autores principales: Alili, Lirija, Chapiro, Swetlana, Marten, Gernot U., Schmidt, Annette M., Zanger, Klaus, Brenneisen, Peter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454731/
https://www.ncbi.nlm.nih.gov/pubmed/26090418
http://dx.doi.org/10.1155/2015/530957
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author Alili, Lirija
Chapiro, Swetlana
Marten, Gernot U.
Schmidt, Annette M.
Zanger, Klaus
Brenneisen, Peter
author_facet Alili, Lirija
Chapiro, Swetlana
Marten, Gernot U.
Schmidt, Annette M.
Zanger, Klaus
Brenneisen, Peter
author_sort Alili, Lirija
collection PubMed
description Iron oxide (Fe(3)O(4)) nanoparticles have been used in many biomedical approaches. The toxicity of Fe(3)O(4) nanoparticles on mammalian cells was published recently. Though, little is known about the viability of human cells after treatment with Fe(3)O(4) nanoparticles. Herein, we examined the toxicity, production of reactive oxygen species, and invasive capacity after treatment of human dermal fibroblasts (HDF) and cells of the squamous tumor cell line (SCL-1) with Fe(3)O(4) nanoparticles. These nanoparticles had an average size of 65 nm. Fe(3)O(4) nanoparticles induced oxidative stress via generation of reactive oxygen species (ROS) and subsequent initiation of lipid peroxidation. Furthermore, the question was addressed of whether Fe(3)O(4) nanoparticles affect myofibroblast formation, known to be involved in tumor invasion. Herein, Fe(3)O(4) nanoparticles prevent the expression alpha-smooth muscle actin and therefore decrease the number of myofibroblastic cells. Moreover, our data show in vitro that concentrations of Fe(3)O(4 ) nanoparticles, which are nontoxic for normal cells, partially reveal a ROS-triggered cytotoxic but also a pro-invasive effect on the fraction of squamous cancer cells surviving the treatment with Fe(3)O(4) nanoparticles. The data herein show that the Fe(3)O(4) nanoparticles appear not to be adequate for use in therapeutic approaches against cancer cells, in contrast to recently published data with cerium oxide nanoparticles.
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spelling pubmed-44547312015-06-18 Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts Alili, Lirija Chapiro, Swetlana Marten, Gernot U. Schmidt, Annette M. Zanger, Klaus Brenneisen, Peter Biomed Res Int Research Article Iron oxide (Fe(3)O(4)) nanoparticles have been used in many biomedical approaches. The toxicity of Fe(3)O(4) nanoparticles on mammalian cells was published recently. Though, little is known about the viability of human cells after treatment with Fe(3)O(4) nanoparticles. Herein, we examined the toxicity, production of reactive oxygen species, and invasive capacity after treatment of human dermal fibroblasts (HDF) and cells of the squamous tumor cell line (SCL-1) with Fe(3)O(4) nanoparticles. These nanoparticles had an average size of 65 nm. Fe(3)O(4) nanoparticles induced oxidative stress via generation of reactive oxygen species (ROS) and subsequent initiation of lipid peroxidation. Furthermore, the question was addressed of whether Fe(3)O(4) nanoparticles affect myofibroblast formation, known to be involved in tumor invasion. Herein, Fe(3)O(4) nanoparticles prevent the expression alpha-smooth muscle actin and therefore decrease the number of myofibroblastic cells. Moreover, our data show in vitro that concentrations of Fe(3)O(4 ) nanoparticles, which are nontoxic for normal cells, partially reveal a ROS-triggered cytotoxic but also a pro-invasive effect on the fraction of squamous cancer cells surviving the treatment with Fe(3)O(4) nanoparticles. The data herein show that the Fe(3)O(4) nanoparticles appear not to be adequate for use in therapeutic approaches against cancer cells, in contrast to recently published data with cerium oxide nanoparticles. Hindawi Publishing Corporation 2015 2015-05-21 /pmc/articles/PMC4454731/ /pubmed/26090418 http://dx.doi.org/10.1155/2015/530957 Text en Copyright © 2015 Lirija Alili et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Alili, Lirija
Chapiro, Swetlana
Marten, Gernot U.
Schmidt, Annette M.
Zanger, Klaus
Brenneisen, Peter
Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts
title Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts
title_full Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts
title_fullStr Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts
title_full_unstemmed Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts
title_short Effect of Fe(3)O(4) Nanoparticles on Skin Tumor Cells and Dermal Fibroblasts
title_sort effect of fe(3)o(4) nanoparticles on skin tumor cells and dermal fibroblasts
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454731/
https://www.ncbi.nlm.nih.gov/pubmed/26090418
http://dx.doi.org/10.1155/2015/530957
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