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From a meso- to micro-scale connectome: array tomography and mGRASP
Mapping mammalian synaptic connectivity has long been an important goal of neuroscience because knowing how neurons and brain areas are connected underpins an understanding of brain function. Meeting this goal requires advanced techniques with single synapse resolution and large-scale capacity, espe...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454886/ https://www.ncbi.nlm.nih.gov/pubmed/26089781 http://dx.doi.org/10.3389/fnana.2015.00078 |
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author | Rah, Jong-Cheol Feng, Linqing Druckmann, Shaul Lee, Hojin Kim, Jinhyun |
author_facet | Rah, Jong-Cheol Feng, Linqing Druckmann, Shaul Lee, Hojin Kim, Jinhyun |
author_sort | Rah, Jong-Cheol |
collection | PubMed |
description | Mapping mammalian synaptic connectivity has long been an important goal of neuroscience because knowing how neurons and brain areas are connected underpins an understanding of brain function. Meeting this goal requires advanced techniques with single synapse resolution and large-scale capacity, especially at multiple scales tethering the meso- and micro-scale connectome. Among several advanced LM-based connectome technologies, Array Tomography (AT) and mammalian GFP-Reconstitution Across Synaptic Partners (mGRASP) can provide relatively high-throughput mapping synaptic connectivity at multiple scales. AT- and mGRASP-assisted circuit mapping (ATing and mGRASPing), combined with techniques such as retrograde virus, brain clearing techniques, and activity indicators will help unlock the secrets of complex neural circuits. Here, we discuss these useful new tools to enable mapping of brain circuits at multiple scales, some functional implications of spatial synaptic distribution, and future challenges and directions of these endeavors. |
format | Online Article Text |
id | pubmed-4454886 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-44548862015-06-18 From a meso- to micro-scale connectome: array tomography and mGRASP Rah, Jong-Cheol Feng, Linqing Druckmann, Shaul Lee, Hojin Kim, Jinhyun Front Neuroanat Neuroscience Mapping mammalian synaptic connectivity has long been an important goal of neuroscience because knowing how neurons and brain areas are connected underpins an understanding of brain function. Meeting this goal requires advanced techniques with single synapse resolution and large-scale capacity, especially at multiple scales tethering the meso- and micro-scale connectome. Among several advanced LM-based connectome technologies, Array Tomography (AT) and mammalian GFP-Reconstitution Across Synaptic Partners (mGRASP) can provide relatively high-throughput mapping synaptic connectivity at multiple scales. AT- and mGRASP-assisted circuit mapping (ATing and mGRASPing), combined with techniques such as retrograde virus, brain clearing techniques, and activity indicators will help unlock the secrets of complex neural circuits. Here, we discuss these useful new tools to enable mapping of brain circuits at multiple scales, some functional implications of spatial synaptic distribution, and future challenges and directions of these endeavors. Frontiers Media S.A. 2015-06-04 /pmc/articles/PMC4454886/ /pubmed/26089781 http://dx.doi.org/10.3389/fnana.2015.00078 Text en Copyright © 2015 Rah, Feng, Druckmann, Lee and Kim. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution and reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Rah, Jong-Cheol Feng, Linqing Druckmann, Shaul Lee, Hojin Kim, Jinhyun From a meso- to micro-scale connectome: array tomography and mGRASP |
title | From a meso- to micro-scale connectome: array tomography and mGRASP |
title_full | From a meso- to micro-scale connectome: array tomography and mGRASP |
title_fullStr | From a meso- to micro-scale connectome: array tomography and mGRASP |
title_full_unstemmed | From a meso- to micro-scale connectome: array tomography and mGRASP |
title_short | From a meso- to micro-scale connectome: array tomography and mGRASP |
title_sort | from a meso- to micro-scale connectome: array tomography and mgrasp |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4454886/ https://www.ncbi.nlm.nih.gov/pubmed/26089781 http://dx.doi.org/10.3389/fnana.2015.00078 |
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