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A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes
Standard stereotaxic reference systems play a key role in human brain studies. Stereotaxic coordinate systems have also been developed for experimental animals including non-human primates, dogs, and rodents. However, they are lacking for other species being relevant in experimental neuroscience inc...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455244/ https://www.ncbi.nlm.nih.gov/pubmed/26089780 http://dx.doi.org/10.3389/fnana.2015.00069 |
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author | Nitzsche, Björn Frey, Stephen Collins, Louis D. Seeger, Johannes Lobsien, Donald Dreyer, Antje Kirsten, Holger Stoffel, Michael H. Fonov, Vladimir S. Boltze, Johannes |
author_facet | Nitzsche, Björn Frey, Stephen Collins, Louis D. Seeger, Johannes Lobsien, Donald Dreyer, Antje Kirsten, Holger Stoffel, Michael H. Fonov, Vladimir S. Boltze, Johannes |
author_sort | Nitzsche, Björn |
collection | PubMed |
description | Standard stereotaxic reference systems play a key role in human brain studies. Stereotaxic coordinate systems have also been developed for experimental animals including non-human primates, dogs, and rodents. However, they are lacking for other species being relevant in experimental neuroscience including sheep. Here, we present a spatial, unbiased ovine brain template with tissue probability maps (TPM) that offer a detailed stereotaxic reference frame for anatomical features and localization of brain areas, thereby enabling inter-individual and cross-study comparability. Three-dimensional data sets from healthy adult Merino sheep (Ovis orientalis aries, 12 ewes and 26 neutered rams) were acquired on a 1.5 T Philips MRI using a T1w sequence. Data were averaged by linear and non-linear registration algorithms. Moreover, animals were subjected to detailed brain volume analysis including examinations with respect to body weight (BW), age, and sex. The created T1w brain template provides an appropriate population-averaged ovine brain anatomy in a spatial standard coordinate system. Additionally, TPM for gray (GM) and white (WM) matter as well as cerebrospinal fluid (CSF) classification enabled automatic prior-based tissue segmentation using statistical parametric mapping (SPM). Overall, a positive correlation of GM volume and BW explained about 15% of the variance of GM while a positive correlation between WM and age was found. Absolute tissue volume differences were not detected, indeed ewes showed significantly more GM per bodyweight as compared to neutered rams. The created framework including spatial brain template and TPM represent a useful tool for unbiased automatic image preprocessing and morphological characterization in sheep. Therefore, the reported results may serve as a starting point for further experimental and/or translational research aiming at in vivo analysis in this species. |
format | Online Article Text |
id | pubmed-4455244 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-44552442015-06-18 A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes Nitzsche, Björn Frey, Stephen Collins, Louis D. Seeger, Johannes Lobsien, Donald Dreyer, Antje Kirsten, Holger Stoffel, Michael H. Fonov, Vladimir S. Boltze, Johannes Front Neuroanat Neuroscience Standard stereotaxic reference systems play a key role in human brain studies. Stereotaxic coordinate systems have also been developed for experimental animals including non-human primates, dogs, and rodents. However, they are lacking for other species being relevant in experimental neuroscience including sheep. Here, we present a spatial, unbiased ovine brain template with tissue probability maps (TPM) that offer a detailed stereotaxic reference frame for anatomical features and localization of brain areas, thereby enabling inter-individual and cross-study comparability. Three-dimensional data sets from healthy adult Merino sheep (Ovis orientalis aries, 12 ewes and 26 neutered rams) were acquired on a 1.5 T Philips MRI using a T1w sequence. Data were averaged by linear and non-linear registration algorithms. Moreover, animals were subjected to detailed brain volume analysis including examinations with respect to body weight (BW), age, and sex. The created T1w brain template provides an appropriate population-averaged ovine brain anatomy in a spatial standard coordinate system. Additionally, TPM for gray (GM) and white (WM) matter as well as cerebrospinal fluid (CSF) classification enabled automatic prior-based tissue segmentation using statistical parametric mapping (SPM). Overall, a positive correlation of GM volume and BW explained about 15% of the variance of GM while a positive correlation between WM and age was found. Absolute tissue volume differences were not detected, indeed ewes showed significantly more GM per bodyweight as compared to neutered rams. The created framework including spatial brain template and TPM represent a useful tool for unbiased automatic image preprocessing and morphological characterization in sheep. Therefore, the reported results may serve as a starting point for further experimental and/or translational research aiming at in vivo analysis in this species. Frontiers Media S.A. 2015-06-04 /pmc/articles/PMC4455244/ /pubmed/26089780 http://dx.doi.org/10.3389/fnana.2015.00069 Text en Copyright © 2015 Nitzsche, Frey, Collins, Seeger, Lobsien, Dreyer, Kirsten, Stoffel, Fonov and Boltze. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Nitzsche, Björn Frey, Stephen Collins, Louis D. Seeger, Johannes Lobsien, Donald Dreyer, Antje Kirsten, Holger Stoffel, Michael H. Fonov, Vladimir S. Boltze, Johannes A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes |
title | A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes |
title_full | A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes |
title_fullStr | A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes |
title_full_unstemmed | A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes |
title_short | A stereotaxic, population-averaged T1w ovine brain atlas including cerebral morphology and tissue volumes |
title_sort | stereotaxic, population-averaged t1w ovine brain atlas including cerebral morphology and tissue volumes |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455244/ https://www.ncbi.nlm.nih.gov/pubmed/26089780 http://dx.doi.org/10.3389/fnana.2015.00069 |
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