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Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study

BACKGROUND: Most of the blood tests aiming for breast cancer screening rely on quantification of a single or few biomarkers. The aim of this study was to evaluate the feasibility of detecting breast cancer by analyzing the total biochemical composition of plasma as well as peripheral blood mononucle...

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Autores principales: Zelig, Udi, Barlev, Eyal, Bar, Omri, Gross, Itai, Flomen, Felix, Mordechai, Shaul, Kapelushnik, Joseph, Nathan, Ilana, Kashtan, Hanoch, Wasserberg, Nir, Madhala-Givon, Osnat
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455613/
https://www.ncbi.nlm.nih.gov/pubmed/25975566
http://dx.doi.org/10.1186/s12885-015-1414-7
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author Zelig, Udi
Barlev, Eyal
Bar, Omri
Gross, Itai
Flomen, Felix
Mordechai, Shaul
Kapelushnik, Joseph
Nathan, Ilana
Kashtan, Hanoch
Wasserberg, Nir
Madhala-Givon, Osnat
author_facet Zelig, Udi
Barlev, Eyal
Bar, Omri
Gross, Itai
Flomen, Felix
Mordechai, Shaul
Kapelushnik, Joseph
Nathan, Ilana
Kashtan, Hanoch
Wasserberg, Nir
Madhala-Givon, Osnat
author_sort Zelig, Udi
collection PubMed
description BACKGROUND: Most of the blood tests aiming for breast cancer screening rely on quantification of a single or few biomarkers. The aim of this study was to evaluate the feasibility of detecting breast cancer by analyzing the total biochemical composition of plasma as well as peripheral blood mononuclear cells (PBMCs) using infrared spectroscopy. METHODS: Blood was collected from 29 patients with confirmed breast cancer and 30 controls with benign or no breast tumors, undergoing screening for breast cancer. PBMCs and plasma were isolated and dried on a zinc selenide slide and measured under a Fourier transform infrared (FTIR) microscope to obtain their infrared absorption spectra. Differences in the spectra of PBMCs and plasma between the groups were analyzed as well as the specific influence of the relevant pathological characteristics of the cancer patients. RESULTS: Several bands in the FTIR spectra of both blood components significantly distinguished patients with and without cancer. Employing feature extraction with quadratic discriminant analysis, a sensitivity of ~90 % and a specificity of ~80 % for breast cancer detection was achieved. These results were confirmed by Monte Carlo cross-validation. Further analysis of the cancer group revealed an influence of several clinical parameters, such as the involvement of lymph nodes, on the infrared spectra, with each blood component affected by different parameters. CONCLUSION: The present preliminary study suggests that FTIR spectroscopy of PBMCs and plasma is a potentially feasible and efficient tool for the early detection of breast neoplasms. An important application of our study is the distinction between benign lesions (considered as part of the non-cancer group) and malignant tumors thus reducing false positive results at screening. Furthermore, the correlation of specific spectral changes with clinical parameters of cancer patients indicates for possible contribution to diagnosis and prognosis.
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spelling pubmed-44556132015-06-05 Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study Zelig, Udi Barlev, Eyal Bar, Omri Gross, Itai Flomen, Felix Mordechai, Shaul Kapelushnik, Joseph Nathan, Ilana Kashtan, Hanoch Wasserberg, Nir Madhala-Givon, Osnat BMC Cancer Research Article BACKGROUND: Most of the blood tests aiming for breast cancer screening rely on quantification of a single or few biomarkers. The aim of this study was to evaluate the feasibility of detecting breast cancer by analyzing the total biochemical composition of plasma as well as peripheral blood mononuclear cells (PBMCs) using infrared spectroscopy. METHODS: Blood was collected from 29 patients with confirmed breast cancer and 30 controls with benign or no breast tumors, undergoing screening for breast cancer. PBMCs and plasma were isolated and dried on a zinc selenide slide and measured under a Fourier transform infrared (FTIR) microscope to obtain their infrared absorption spectra. Differences in the spectra of PBMCs and plasma between the groups were analyzed as well as the specific influence of the relevant pathological characteristics of the cancer patients. RESULTS: Several bands in the FTIR spectra of both blood components significantly distinguished patients with and without cancer. Employing feature extraction with quadratic discriminant analysis, a sensitivity of ~90 % and a specificity of ~80 % for breast cancer detection was achieved. These results were confirmed by Monte Carlo cross-validation. Further analysis of the cancer group revealed an influence of several clinical parameters, such as the involvement of lymph nodes, on the infrared spectra, with each blood component affected by different parameters. CONCLUSION: The present preliminary study suggests that FTIR spectroscopy of PBMCs and plasma is a potentially feasible and efficient tool for the early detection of breast neoplasms. An important application of our study is the distinction between benign lesions (considered as part of the non-cancer group) and malignant tumors thus reducing false positive results at screening. Furthermore, the correlation of specific spectral changes with clinical parameters of cancer patients indicates for possible contribution to diagnosis and prognosis. BioMed Central 2015-05-15 /pmc/articles/PMC4455613/ /pubmed/25975566 http://dx.doi.org/10.1186/s12885-015-1414-7 Text en © Zelig et al.; licensee BioMed Central. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Zelig, Udi
Barlev, Eyal
Bar, Omri
Gross, Itai
Flomen, Felix
Mordechai, Shaul
Kapelushnik, Joseph
Nathan, Ilana
Kashtan, Hanoch
Wasserberg, Nir
Madhala-Givon, Osnat
Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study
title Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study
title_full Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study
title_fullStr Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study
title_full_unstemmed Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study
title_short Early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study
title_sort early detection of breast cancer using total biochemical analysis of peripheral blood components: a preliminary study
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455613/
https://www.ncbi.nlm.nih.gov/pubmed/25975566
http://dx.doi.org/10.1186/s12885-015-1414-7
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