A strategy for enhanced circular DNA construction efficiency based on DNA cyclization after microbial transformation

BACKGROUND: With the rapid development of synthetic biology, the demand for assembling multiple DNA (genes) fragments into a large circular DNA structure in one step has dramatically increased. However, for constructions of most circular DNA, there are two contradictions in the ligation/assembly and...

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Detalles Bibliográficos
Autores principales: Guo, Ying-Ying, Shi, Zhen-Yu, Fu, Xiao-Zhi, Chen, Jin-Chun, Wu, Qiong, Chen, Guo-Qiang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455692/
https://www.ncbi.nlm.nih.gov/pubmed/25896825
http://dx.doi.org/10.1186/s12934-015-0204-x

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