RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein

We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detecti...

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Detalles Bibliográficos
Autores principales: Lee, Jeong-Soo, Kwak, Su-Jin, Kim, Jungeun, Kim, Ae-Kyeong, Noh, Hae Min, Kim, Jin-Soo, Yu, Kweon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2014
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455777/
https://www.ncbi.nlm.nih.gov/pubmed/24875628
http://dx.doi.org/10.1534/g3.114.012179
Descripción
Sumario:We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.

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