RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein

We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detecti...

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Detalles Bibliográficos
Autores principales: Lee, Jeong-Soo, Kwak, Su-Jin, Kim, Jungeun, Kim, Ae-Kyeong, Noh, Hae Min, Kim, Jin-Soo, Yu, Kweon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Genetics Society of America 2014
Materias:
Investigations
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455777/
https://www.ncbi.nlm.nih.gov/pubmed/24875628
http://dx.doi.org/10.1534/g3.114.012179
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author Lee, Jeong-Soo
Kwak, Su-Jin
Kim, Jungeun
Kim, Ae-Kyeong
Noh, Hae Min
Kim, Jin-Soo
Yu, Kweon
author_facet Lee, Jeong-Soo
Kwak, Su-Jin
Kim, Jungeun
Kim, Ae-Kyeong
Noh, Hae Min
Kim, Jin-Soo
Yu, Kweon
author_sort Lee, Jeong-Soo
collection PubMed
description We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay.
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spelling pubmed-44557772015-06-08 RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein Lee, Jeong-Soo Kwak, Su-Jin Kim, Jungeun Kim, Ae-Kyeong Noh, Hae Min Kim, Jin-Soo Yu, Kweon G3 (Bethesda) Investigations We report a method for generating Drosophila germline mutants effectively via injection of the complex of the purified Cas9 protein, tracrRNA, and gene-specific crRNAs, which may reduce delayed mutations because of the transient activity of the Cas9 protein, combined with the simple mutation detection in GO founders by the T7E1 assay. Genetics Society of America 2014-07-01 /pmc/articles/PMC4455777/ /pubmed/24875628 http://dx.doi.org/10.1534/g3.114.012179 Text en Copyright © 2014 Lee et al. http://creativecommons.org/licenses/by/3.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution Unported License (http://creativecommons.org/licenses/by/3.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Investigations
Lee, Jeong-Soo
Kwak, Su-Jin
Kim, Jungeun
Kim, Ae-Kyeong
Noh, Hae Min
Kim, Jin-Soo
Yu, Kweon
RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
title RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
title_full RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
title_fullStr RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
title_full_unstemmed RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
title_short RNA-Guided Genome Editing in Drosophila with the Purified Cas9 Protein
title_sort rna-guided genome editing in drosophila with the purified cas9 protein
topic Investigations
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455777/
https://www.ncbi.nlm.nih.gov/pubmed/24875628
http://dx.doi.org/10.1534/g3.114.012179
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