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Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism
The present study aims to elucidate the roles of nitric oxide synthase activity, oxidative stress, inflammation, and apoptosis in penile toxicity of aging mice associated with excess manganese (Mn) treatment and to investigate the effect of oral treatment with the herbal formula B401 in this respect...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove Medical Press
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455845/ https://www.ncbi.nlm.nih.gov/pubmed/26064043 http://dx.doi.org/10.2147/CIA.S82026 |
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author | Hsu, Chih-Hsiang Lin, Ching-Lung Wang, Sheue-Er Sheu, Shuenn-Jyi Chien, Chiang-Ting Wu, Chung-Hsin |
author_facet | Hsu, Chih-Hsiang Lin, Ching-Lung Wang, Sheue-Er Sheu, Shuenn-Jyi Chien, Chiang-Ting Wu, Chung-Hsin |
author_sort | Hsu, Chih-Hsiang |
collection | PubMed |
description | The present study aims to elucidate the roles of nitric oxide synthase activity, oxidative stress, inflammation, and apoptosis in penile toxicity of aging mice associated with excess manganese (Mn) treatment and to investigate the effect of oral treatment with the herbal formula B401 in this respect. ICR strain mice were divided into two groups: the vehicle (sham group) and the B401 (50 mg/kg) group. The mice were orally treated for 5 days; then a high single dose of MnCl(2) (100 mg/kg) was given by intraperitoneal injection to the mice. One day after MnCl(2) treatment, corpora cavernosal tissues of both Mn-treated mice and their controls were simultaneously sampled to examine their immunohistochemical staining and Western blot analysis. Nitric oxide (NO) production, levels of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), expression levels of factors governing angiogenesis (vascular endothelial growth factor), oxidative stress (catalase, superoxide dismutase 2,4-hydroxynonenal), inflammation (tumor necrosis factor alpha), apoptosis (B-cell lymphoma 2 [Bcl-2], Bcl-2-associated X protein [Bax], cleaved poly(adenosine diphosphate-ribose) polymerase [c-PARP], cytochrome C, caspase-12, and caspase-3) were evaluated in penile corpus cavernosum of the mice. We found that penile toxicity in the mice was enhanced under excess Mn treatment through reduction of NOS activity and increase in oxidative stress, inflammation, and apoptosis in the penile cavernous tissue. Furthermore, the penile toxicity in mice with manganism was alleviated by oral B401 treatment through enhancement of both nitric oxide synthesis and angiogenesis, with simultaneous reduction of oxidative stress, inflammation, and apoptosis in penile corpus cavernosum. We suggest that the herbal formula B401 may serve as a potential dietotherapeutic supplement for penile toxicity or dysfunction in aging males. |
format | Online Article Text |
id | pubmed-4455845 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Dove Medical Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-44558452015-06-10 Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism Hsu, Chih-Hsiang Lin, Ching-Lung Wang, Sheue-Er Sheu, Shuenn-Jyi Chien, Chiang-Ting Wu, Chung-Hsin Clin Interv Aging Original Research The present study aims to elucidate the roles of nitric oxide synthase activity, oxidative stress, inflammation, and apoptosis in penile toxicity of aging mice associated with excess manganese (Mn) treatment and to investigate the effect of oral treatment with the herbal formula B401 in this respect. ICR strain mice were divided into two groups: the vehicle (sham group) and the B401 (50 mg/kg) group. The mice were orally treated for 5 days; then a high single dose of MnCl(2) (100 mg/kg) was given by intraperitoneal injection to the mice. One day after MnCl(2) treatment, corpora cavernosal tissues of both Mn-treated mice and their controls were simultaneously sampled to examine their immunohistochemical staining and Western blot analysis. Nitric oxide (NO) production, levels of neuronal nitric oxide synthase (nNOS) and endothelial nitric oxide synthase (eNOS), expression levels of factors governing angiogenesis (vascular endothelial growth factor), oxidative stress (catalase, superoxide dismutase 2,4-hydroxynonenal), inflammation (tumor necrosis factor alpha), apoptosis (B-cell lymphoma 2 [Bcl-2], Bcl-2-associated X protein [Bax], cleaved poly(adenosine diphosphate-ribose) polymerase [c-PARP], cytochrome C, caspase-12, and caspase-3) were evaluated in penile corpus cavernosum of the mice. We found that penile toxicity in the mice was enhanced under excess Mn treatment through reduction of NOS activity and increase in oxidative stress, inflammation, and apoptosis in the penile cavernous tissue. Furthermore, the penile toxicity in mice with manganism was alleviated by oral B401 treatment through enhancement of both nitric oxide synthesis and angiogenesis, with simultaneous reduction of oxidative stress, inflammation, and apoptosis in penile corpus cavernosum. We suggest that the herbal formula B401 may serve as a potential dietotherapeutic supplement for penile toxicity or dysfunction in aging males. Dove Medical Press 2015-05-28 /pmc/articles/PMC4455845/ /pubmed/26064043 http://dx.doi.org/10.2147/CIA.S82026 Text en © 2015 Hsu et al. This work is published by Dove Medical Press Limited, and licensed under Creative Commons Attribution – Non Commercial (unported, v3.0) License The full terms of the License are available at http://creativecommons.org/licenses/by-nc/3.0/. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. |
spellingShingle | Original Research Hsu, Chih-Hsiang Lin, Ching-Lung Wang, Sheue-Er Sheu, Shuenn-Jyi Chien, Chiang-Ting Wu, Chung-Hsin Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism |
title | Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism |
title_full | Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism |
title_fullStr | Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism |
title_full_unstemmed | Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism |
title_short | Oral treatment with herbal formula B401 alleviates penile toxicity in aging mice with manganism |
title_sort | oral treatment with herbal formula b401 alleviates penile toxicity in aging mice with manganism |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455845/ https://www.ncbi.nlm.nih.gov/pubmed/26064043 http://dx.doi.org/10.2147/CIA.S82026 |
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