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microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma

BACKGROUND: microRNAs (miRNAs) have been reported to modulate macrophage colony-stimulating factor (M-CSF) and macrophages. The aim of this study was to find whether miR-26a can suppress M-CSF expression and the recruitment of macrophages. METHODS: Hepatocellular carcinoma (HCC) cell lines with decr...

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Autores principales: Chai, Zong-Tao, Zhu, Xiao-Dong, Ao, Jian-Yang, Wang, Wen-Quan, Gao, Dong-Mei, Kong, Jian, Zhang, Ning, Zhang, Yuan-Yuan, Ye, Bo-Gen, Ma, De-Ning, Cai, Hao, Sun, Hui-Chuan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455972/
https://www.ncbi.nlm.nih.gov/pubmed/26021873
http://dx.doi.org/10.1186/s13045-015-0150-4
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author Chai, Zong-Tao
Zhu, Xiao-Dong
Ao, Jian-Yang
Wang, Wen-Quan
Gao, Dong-Mei
Kong, Jian
Zhang, Ning
Zhang, Yuan-Yuan
Ye, Bo-Gen
Ma, De-Ning
Cai, Hao
Sun, Hui-Chuan
author_facet Chai, Zong-Tao
Zhu, Xiao-Dong
Ao, Jian-Yang
Wang, Wen-Quan
Gao, Dong-Mei
Kong, Jian
Zhang, Ning
Zhang, Yuan-Yuan
Ye, Bo-Gen
Ma, De-Ning
Cai, Hao
Sun, Hui-Chuan
author_sort Chai, Zong-Tao
collection PubMed
description BACKGROUND: microRNAs (miRNAs) have been reported to modulate macrophage colony-stimulating factor (M-CSF) and macrophages. The aim of this study was to find whether miR-26a can suppress M-CSF expression and the recruitment of macrophages. METHODS: Hepatocellular carcinoma (HCC) cell lines with decreased or increased expression of miR-26a were established in a previous study. M-CSF expression by tumor cells was measured by enzyme-linked immunosorbent assay, and cell migration assays were used to explore the effect of HCC cell lines on macrophage recruitment in vitro. Real-time PCR measured a panel of mRNAs expressed by macrophages. Xenograft models were used to observe tumor growth. Immunohistochemistry was conducted to study the relation between miR-26a expression and M-CSF expression and macrophage recruitment in patients with HCC. RESULTS: Ectopic expression of miR-26a reduced expression of M-CSF. The conditioned medium (CM) from HepG2 cells that overexpressed miR-26a reduced the migration ability of THP-1 cells stimulated by phorbol myristate acetate (PMA) increased expression of interleukin (IL)-12b or IL-23 mRNA and decreased expression of chemokine (C-C motif) ligand (CCL)22, CCL17, and IL-10 mRNA, in comparison to the medium from the parental HepG2 cells. These effects could be interrupted by the PI3K/Akt pathway inhibitor LY294002. Ectopic expression of miR-26a in HCC cells suppressed tumor growth, M-CSF expression, and infiltration of macrophages in tumors. Similar results were also found when using HCCLM3 cells. Furthermore, the expression of miR-26a was inversely correlated with M-CSF expression and macrophage infiltration in tumor tissues from patients with HCC. CONCLUSIONS: miR-26a expression reduced M-CSF expression and recruitment of macrophages in HCC.
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spelling pubmed-44559722015-06-05 microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma Chai, Zong-Tao Zhu, Xiao-Dong Ao, Jian-Yang Wang, Wen-Quan Gao, Dong-Mei Kong, Jian Zhang, Ning Zhang, Yuan-Yuan Ye, Bo-Gen Ma, De-Ning Cai, Hao Sun, Hui-Chuan J Hematol Oncol Research Article BACKGROUND: microRNAs (miRNAs) have been reported to modulate macrophage colony-stimulating factor (M-CSF) and macrophages. The aim of this study was to find whether miR-26a can suppress M-CSF expression and the recruitment of macrophages. METHODS: Hepatocellular carcinoma (HCC) cell lines with decreased or increased expression of miR-26a were established in a previous study. M-CSF expression by tumor cells was measured by enzyme-linked immunosorbent assay, and cell migration assays were used to explore the effect of HCC cell lines on macrophage recruitment in vitro. Real-time PCR measured a panel of mRNAs expressed by macrophages. Xenograft models were used to observe tumor growth. Immunohistochemistry was conducted to study the relation between miR-26a expression and M-CSF expression and macrophage recruitment in patients with HCC. RESULTS: Ectopic expression of miR-26a reduced expression of M-CSF. The conditioned medium (CM) from HepG2 cells that overexpressed miR-26a reduced the migration ability of THP-1 cells stimulated by phorbol myristate acetate (PMA) increased expression of interleukin (IL)-12b or IL-23 mRNA and decreased expression of chemokine (C-C motif) ligand (CCL)22, CCL17, and IL-10 mRNA, in comparison to the medium from the parental HepG2 cells. These effects could be interrupted by the PI3K/Akt pathway inhibitor LY294002. Ectopic expression of miR-26a in HCC cells suppressed tumor growth, M-CSF expression, and infiltration of macrophages in tumors. Similar results were also found when using HCCLM3 cells. Furthermore, the expression of miR-26a was inversely correlated with M-CSF expression and macrophage infiltration in tumor tissues from patients with HCC. CONCLUSIONS: miR-26a expression reduced M-CSF expression and recruitment of macrophages in HCC. BioMed Central 2015-05-29 /pmc/articles/PMC4455972/ /pubmed/26021873 http://dx.doi.org/10.1186/s13045-015-0150-4 Text en © Chai et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Chai, Zong-Tao
Zhu, Xiao-Dong
Ao, Jian-Yang
Wang, Wen-Quan
Gao, Dong-Mei
Kong, Jian
Zhang, Ning
Zhang, Yuan-Yuan
Ye, Bo-Gen
Ma, De-Ning
Cai, Hao
Sun, Hui-Chuan
microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma
title microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma
title_full microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma
title_fullStr microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma
title_full_unstemmed microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma
title_short microRNA-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the PI3K/Akt pathway in hepatocellular carcinoma
title_sort microrna-26a suppresses recruitment of macrophages by down-regulating macrophage colony-stimulating factor expression through the pi3k/akt pathway in hepatocellular carcinoma
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4455972/
https://www.ncbi.nlm.nih.gov/pubmed/26021873
http://dx.doi.org/10.1186/s13045-015-0150-4
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