Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach

BACKGROUND: Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. METHODS: A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercop...

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Autores principales: Marangi, Marianna, Koehler, Anson V, Zanzani, Sergio A, Manfredi, Maria T, Brianti, Emanuele, Giangaspero, Annunziata, Gasser, Robin B
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Research
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456053/
https://www.ncbi.nlm.nih.gov/pubmed/25972100
http://dx.doi.org/10.1186/s13071-015-0872-8
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author Marangi, Marianna
Koehler, Anson V
Zanzani, Sergio A
Manfredi, Maria T
Brianti, Emanuele
Giangaspero, Annunziata
Gasser, Robin B
author_facet Marangi, Marianna
Koehler, Anson V
Zanzani, Sergio A
Manfredi, Maria T
Brianti, Emanuele
Giangaspero, Annunziata
Gasser, Robin B
author_sort Marangi, Marianna
collection PubMed
description BACKGROUND: Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. METHODS: A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. RESULTS: Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. CONCLUSIONS: The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans.
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spelling pubmed-44560532015-06-05 Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach Marangi, Marianna Koehler, Anson V Zanzani, Sergio A Manfredi, Maria T Brianti, Emanuele Giangaspero, Annunziata Gasser, Robin B Parasit Vectors Research BACKGROUND: Cyclospora is a protistan parasite that causes enteritis in several species of animals including humans. The aim of this study was to investigate the presence of Cyclospora in captive non-human primates. METHODS: A total of 119 faecal samples from Pan troglodytes, Macaca sylvanus, Cercopithecus cephus, Erythrocebus patas, Chlorocebus aethiops and Macaca fascicularis from a wildlife animal rescue center as well as from Macaca fascicularis from an experimental primate research center were tested for the presence of Cyclospora by quantitative real-time PCR (qPCR) and single-strand conformation polymorphism (SSCP) analysis. RESULTS: Cyclospora was detected in three Pan troglodytes (13.6%) and nine (9.3%) Macaca fascicularis. CONCLUSIONS: The present study represents the first record of Cyclospora in captive primates in Europe, suggesting the presence of Cyclospora cayetanensis, which is transmissible to humans. BioMed Central 2015-05-15 /pmc/articles/PMC4456053/ /pubmed/25972100 http://dx.doi.org/10.1186/s13071-015-0872-8 Text en © Marangi et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Marangi, Marianna
Koehler, Anson V
Zanzani, Sergio A
Manfredi, Maria T
Brianti, Emanuele
Giangaspero, Annunziata
Gasser, Robin B
Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
title Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
title_full Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
title_fullStr Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
title_full_unstemmed Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
title_short Detection of Cyclospora in captive chimpanzees and macaques by a quantitative PCR-based mutation scanning approach
title_sort detection of cyclospora in captive chimpanzees and macaques by a quantitative pcr-based mutation scanning approach
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456053/
https://www.ncbi.nlm.nih.gov/pubmed/25972100
http://dx.doi.org/10.1186/s13071-015-0872-8
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