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Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants

For the development of “medical foods” and/or botanical drugs as defined USA FDA, clear and systemic characterizations of the taxonomy, index phytochemical components, and the functional or medicinal bioactivities of the reputed or candidate medicinal plant are needed. In this study, we used an inte...

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Autores principales: Lin, Wen-Ching, Wen, Chih-Chun, Chen, Yung-Hsiang, Hsiao, Pei-Wen, Liao, Jiunn-Wang, Peng, Ching-I, Yang, Ning-Sun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456162/
https://www.ncbi.nlm.nih.gov/pubmed/26042672
http://dx.doi.org/10.1371/journal.pone.0129067
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author Lin, Wen-Ching
Wen, Chih-Chun
Chen, Yung-Hsiang
Hsiao, Pei-Wen
Liao, Jiunn-Wang
Peng, Ching-I
Yang, Ning-Sun
author_facet Lin, Wen-Ching
Wen, Chih-Chun
Chen, Yung-Hsiang
Hsiao, Pei-Wen
Liao, Jiunn-Wang
Peng, Ching-I
Yang, Ning-Sun
author_sort Lin, Wen-Ching
collection PubMed
description For the development of “medical foods” and/or botanical drugs as defined USA FDA, clear and systemic characterizations of the taxonomy, index phytochemical components, and the functional or medicinal bioactivities of the reputed or candidate medicinal plant are needed. In this study, we used an integrative approach, including macroscopic and microscopic examination, marker gene analysis, and chemical fingerprinting, to authenticate and validate various species/varieties of Wedelia, a reputed medicinal plant that grows naturally and commonly used in Asian countries. The anti-inflammatory bioactivities of Wedelia extracts were then evaluated in a DSS-induced murine colitis model. Different species/varieties of Wedelia exhibited distinguishable morphology and histological structures. Analysis of the ribosomal DNA internal transcribed spacer (ITS) region revealed significant differences among these plants. Chemical profiling of test Wedelia species demonstrated candidate index compounds and distinguishable secondary metabolites, such as caffeic acid derivatives, which may serve as phytochemical markers or index for quality control and identification of specific Wedelia species. In assessing their effect on treating DSS induced-murine colitis, we observed that only the phytoextract from W. chinensis species exhibited significant anti-inflammatory bioactivity on DSS-induced murine colitis among the various Wedelia species commonly found in Taiwan. Our results provide a translational research approach that may serve as a useful reference platform for biotechnological applications of traditional phytomedicines. Our findings indicate that specific Wedelia species warrant further investigation for potential treatment of human inflammatory bowel disease.
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spelling pubmed-44561622015-06-09 Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants Lin, Wen-Ching Wen, Chih-Chun Chen, Yung-Hsiang Hsiao, Pei-Wen Liao, Jiunn-Wang Peng, Ching-I Yang, Ning-Sun PLoS One Research Article For the development of “medical foods” and/or botanical drugs as defined USA FDA, clear and systemic characterizations of the taxonomy, index phytochemical components, and the functional or medicinal bioactivities of the reputed or candidate medicinal plant are needed. In this study, we used an integrative approach, including macroscopic and microscopic examination, marker gene analysis, and chemical fingerprinting, to authenticate and validate various species/varieties of Wedelia, a reputed medicinal plant that grows naturally and commonly used in Asian countries. The anti-inflammatory bioactivities of Wedelia extracts were then evaluated in a DSS-induced murine colitis model. Different species/varieties of Wedelia exhibited distinguishable morphology and histological structures. Analysis of the ribosomal DNA internal transcribed spacer (ITS) region revealed significant differences among these plants. Chemical profiling of test Wedelia species demonstrated candidate index compounds and distinguishable secondary metabolites, such as caffeic acid derivatives, which may serve as phytochemical markers or index for quality control and identification of specific Wedelia species. In assessing their effect on treating DSS induced-murine colitis, we observed that only the phytoextract from W. chinensis species exhibited significant anti-inflammatory bioactivity on DSS-induced murine colitis among the various Wedelia species commonly found in Taiwan. Our results provide a translational research approach that may serve as a useful reference platform for biotechnological applications of traditional phytomedicines. Our findings indicate that specific Wedelia species warrant further investigation for potential treatment of human inflammatory bowel disease. Public Library of Science 2015-06-04 /pmc/articles/PMC4456162/ /pubmed/26042672 http://dx.doi.org/10.1371/journal.pone.0129067 Text en © 2015 Lin et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Lin, Wen-Ching
Wen, Chih-Chun
Chen, Yung-Hsiang
Hsiao, Pei-Wen
Liao, Jiunn-Wang
Peng, Ching-I
Yang, Ning-Sun
Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants
title Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants
title_full Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants
title_fullStr Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants
title_full_unstemmed Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants
title_short Integrative Approach to Analyze Biodiversity and Anti-Inflammatory Bioactivity of Wedelia Medicinal Plants
title_sort integrative approach to analyze biodiversity and anti-inflammatory bioactivity of wedelia medicinal plants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456162/
https://www.ncbi.nlm.nih.gov/pubmed/26042672
http://dx.doi.org/10.1371/journal.pone.0129067
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