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The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components

Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view o...

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Autores principales: Aeberhard, Lukas, Banhart, Sebastian, Fischer, Martina, Jehmlich, Nico, Rose, Laura, Koch, Sophia, Laue, Michael, Renard, Bernhard Y., Schmidt, Frank, Heuer, Dagmar
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456400/
https://www.ncbi.nlm.nih.gov/pubmed/26042774
http://dx.doi.org/10.1371/journal.ppat.1004883
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author Aeberhard, Lukas
Banhart, Sebastian
Fischer, Martina
Jehmlich, Nico
Rose, Laura
Koch, Sophia
Laue, Michael
Renard, Bernhard Y.
Schmidt, Frank
Heuer, Dagmar
author_facet Aeberhard, Lukas
Banhart, Sebastian
Fischer, Martina
Jehmlich, Nico
Rose, Laura
Koch, Sophia
Laue, Michael
Renard, Bernhard Y.
Schmidt, Frank
Heuer, Dagmar
author_sort Aeberhard, Lukas
collection PubMed
description Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe the host cell-derived proteome of isolated C. trachomatis inclusions by quantitative proteomics. Computational analysis indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells’ antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular, SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, these findings suggest that C. trachomatis hijacks retrograde pathways for effective infection.
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spelling pubmed-44564002015-06-09 The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components Aeberhard, Lukas Banhart, Sebastian Fischer, Martina Jehmlich, Nico Rose, Laura Koch, Sophia Laue, Michael Renard, Bernhard Y. Schmidt, Frank Heuer, Dagmar PLoS Pathog Research Article Chlamydia trachomatis is an important human pathogen that replicates inside the infected host cell in a unique vacuole, the inclusion. The formation of this intracellular bacterial niche is essential for productive Chlamydia infections. Despite its importance for Chlamydia biology, a holistic view on the protein composition of the inclusion, including its membrane, is currently missing. Here we describe the host cell-derived proteome of isolated C. trachomatis inclusions by quantitative proteomics. Computational analysis indicated that the inclusion is a complex intracellular trafficking platform that interacts with host cells’ antero- and retrograde trafficking pathways. Furthermore, the inclusion is highly enriched for sorting nexins of the SNX-BAR retromer, a complex essential for retrograde trafficking. Functional studies showed that in particular, SNX5 controls the C. trachomatis infection and that retrograde trafficking is essential for infectious progeny formation. In summary, these findings suggest that C. trachomatis hijacks retrograde pathways for effective infection. Public Library of Science 2015-06-04 /pmc/articles/PMC4456400/ /pubmed/26042774 http://dx.doi.org/10.1371/journal.ppat.1004883 Text en © 2015 Aeberhard et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Aeberhard, Lukas
Banhart, Sebastian
Fischer, Martina
Jehmlich, Nico
Rose, Laura
Koch, Sophia
Laue, Michael
Renard, Bernhard Y.
Schmidt, Frank
Heuer, Dagmar
The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
title The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
title_full The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
title_fullStr The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
title_full_unstemmed The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
title_short The Proteome of the Isolated Chlamydia trachomatis Containing Vacuole Reveals a Complex Trafficking Platform Enriched for Retromer Components
title_sort proteome of the isolated chlamydia trachomatis containing vacuole reveals a complex trafficking platform enriched for retromer components
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456400/
https://www.ncbi.nlm.nih.gov/pubmed/26042774
http://dx.doi.org/10.1371/journal.ppat.1004883
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