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Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender

BACKGROUND AND AIMS: We have reported that adverse effects on flow-mediated dilation of an acute elevation of non-esterified fatty acids rich in saturated fat (SFA) are reversed following addition of long-chain (LC) n-3 polyunsaturated fatty acids (PUFA), and hypothesised that these effects may be m...

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Autores principales: Newens, K.J., Thompson, A.K., Jackson, K.G., Williams, C.M.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456421/
https://www.ncbi.nlm.nih.gov/pubmed/25921849
http://dx.doi.org/10.1016/j.numecd.2015.03.004
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author Newens, K.J.
Thompson, A.K.
Jackson, K.G.
Williams, C.M.
author_facet Newens, K.J.
Thompson, A.K.
Jackson, K.G.
Williams, C.M.
author_sort Newens, K.J.
collection PubMed
description BACKGROUND AND AIMS: We have reported that adverse effects on flow-mediated dilation of an acute elevation of non-esterified fatty acids rich in saturated fat (SFA) are reversed following addition of long-chain (LC) n-3 polyunsaturated fatty acids (PUFA), and hypothesised that these effects may be mediated through alterations in insulin signalling pathways. In a subgroup, we explored the effects of raised NEFA enriched with SFA, with or without LC n-3 PUFA, on whole body insulin sensitivity (SI) and responsiveness of the endothelium to insulin infusion. METHODS AND RESULTS: Thirty adults (mean age 27.8 y, BMI 23.2 kg/m(2)) consumed oral fat loads on separate occasions with continuous heparin infusion to elevate NEFA between 60 and 390 min. For the final 150 min, a hyperinsulinaemic-euglycaemic clamp was performed, whilst FMD and circulating markers of endothelial function were measured at baseline, pre-clamp (240 min) and post-clamp (390 min). NEFA elevation during the SFA-rich drinks was associated with impaired FMD (P = 0.027) whilst SFA + LC n-3 PUFA improved FMD at 240 min (P = 0.003). In males, insulin infusion attenuated the increase in FMD with SFA + LC n-3 PUFA (P = 0.049), with SI 10% greater with SFA + LC n-3 PUFA than SFA (P = 0.041). CONCLUSION: This study provides evidence that NEFA composition during acute elevation influences both FMD and SI, with some indication of a difference by gender. However our findings are not consistent with the hypothesis that the effects of fatty acids on endothelial function and SI operate through a common pathway. This trial was registered at clinical trials.gov as NCT01351324 on 6th May 2011.
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spelling pubmed-44564212015-06-14 Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender Newens, K.J. Thompson, A.K. Jackson, K.G. Williams, C.M. Nutr Metab Cardiovasc Dis Article BACKGROUND AND AIMS: We have reported that adverse effects on flow-mediated dilation of an acute elevation of non-esterified fatty acids rich in saturated fat (SFA) are reversed following addition of long-chain (LC) n-3 polyunsaturated fatty acids (PUFA), and hypothesised that these effects may be mediated through alterations in insulin signalling pathways. In a subgroup, we explored the effects of raised NEFA enriched with SFA, with or without LC n-3 PUFA, on whole body insulin sensitivity (SI) and responsiveness of the endothelium to insulin infusion. METHODS AND RESULTS: Thirty adults (mean age 27.8 y, BMI 23.2 kg/m(2)) consumed oral fat loads on separate occasions with continuous heparin infusion to elevate NEFA between 60 and 390 min. For the final 150 min, a hyperinsulinaemic-euglycaemic clamp was performed, whilst FMD and circulating markers of endothelial function were measured at baseline, pre-clamp (240 min) and post-clamp (390 min). NEFA elevation during the SFA-rich drinks was associated with impaired FMD (P = 0.027) whilst SFA + LC n-3 PUFA improved FMD at 240 min (P = 0.003). In males, insulin infusion attenuated the increase in FMD with SFA + LC n-3 PUFA (P = 0.049), with SI 10% greater with SFA + LC n-3 PUFA than SFA (P = 0.041). CONCLUSION: This study provides evidence that NEFA composition during acute elevation influences both FMD and SI, with some indication of a difference by gender. However our findings are not consistent with the hypothesis that the effects of fatty acids on endothelial function and SI operate through a common pathway. This trial was registered at clinical trials.gov as NCT01351324 on 6th May 2011. Elsevier 2015-06 /pmc/articles/PMC4456421/ /pubmed/25921849 http://dx.doi.org/10.1016/j.numecd.2015.03.004 Text en © 2015 Elsevier B.V. All rights reserved. https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Newens, K.J.
Thompson, A.K.
Jackson, K.G.
Williams, C.M.
Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender
title Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender
title_full Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender
title_fullStr Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender
title_full_unstemmed Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender
title_short Endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: Effects of fat composition and gender
title_sort endothelial function and insulin sensitivity during acute non-esterified fatty acid elevation: effects of fat composition and gender
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456421/
https://www.ncbi.nlm.nih.gov/pubmed/25921849
http://dx.doi.org/10.1016/j.numecd.2015.03.004
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