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In vivo imaging of CREB phosphorylation in awake-mouse brain

The cyclic adenosine monophosphate response element binding protein (CREB) is a phosphorylation-dependent transcription factor that plays important roles in memory consolidation and several neuropsychological disorders. Although analyzing the spatiotemporal pattern of CREB phosphorylation is require...

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Detalles Bibliográficos
Autores principales: Ishimoto, Tetsuya, Mano, Hiroki, Mori, Hisashi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456726/
https://www.ncbi.nlm.nih.gov/pubmed/26044058
http://dx.doi.org/10.1038/srep09757
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author Ishimoto, Tetsuya
Mano, Hiroki
Mori, Hisashi
author_facet Ishimoto, Tetsuya
Mano, Hiroki
Mori, Hisashi
author_sort Ishimoto, Tetsuya
collection PubMed
description The cyclic adenosine monophosphate response element binding protein (CREB) is a phosphorylation-dependent transcription factor that plays important roles in memory consolidation and several neuropsychological disorders. Although analyzing the spatiotemporal pattern of CREB phosphorylation is required for elucidating the mechanism of memory consolidation, imaging of phosphorylation of a particular protein in the brain of live animals is impossible at present. Here, we developed a method for visualizing the CREB phosphorylation in the cerebral cortex of an awake mouse using a split luciferase technique. Using this technique, we demonstrated the correlation between the change in CREB phosphorylation at a particular region in the brain and behavioral consequences induced by the administration of reserpine, a psychotropic agent.
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spelling pubmed-44567262015-06-12 In vivo imaging of CREB phosphorylation in awake-mouse brain Ishimoto, Tetsuya Mano, Hiroki Mori, Hisashi Sci Rep Article The cyclic adenosine monophosphate response element binding protein (CREB) is a phosphorylation-dependent transcription factor that plays important roles in memory consolidation and several neuropsychological disorders. Although analyzing the spatiotemporal pattern of CREB phosphorylation is required for elucidating the mechanism of memory consolidation, imaging of phosphorylation of a particular protein in the brain of live animals is impossible at present. Here, we developed a method for visualizing the CREB phosphorylation in the cerebral cortex of an awake mouse using a split luciferase technique. Using this technique, we demonstrated the correlation between the change in CREB phosphorylation at a particular region in the brain and behavioral consequences induced by the administration of reserpine, a psychotropic agent. Nature Publishing Group 2015-06-05 /pmc/articles/PMC4456726/ /pubmed/26044058 http://dx.doi.org/10.1038/srep09757 Text en Copyright © 2015, Macmillan Publishers Limited. All rights reserved http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder in order to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Ishimoto, Tetsuya
Mano, Hiroki
Mori, Hisashi
In vivo imaging of CREB phosphorylation in awake-mouse brain
title In vivo imaging of CREB phosphorylation in awake-mouse brain
title_full In vivo imaging of CREB phosphorylation in awake-mouse brain
title_fullStr In vivo imaging of CREB phosphorylation in awake-mouse brain
title_full_unstemmed In vivo imaging of CREB phosphorylation in awake-mouse brain
title_short In vivo imaging of CREB phosphorylation in awake-mouse brain
title_sort in vivo imaging of creb phosphorylation in awake-mouse brain
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456726/
https://www.ncbi.nlm.nih.gov/pubmed/26044058
http://dx.doi.org/10.1038/srep09757
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