Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology

We describe a modular culture platform that enables spatiotemporal control of the morphology of 2D neural tissues derived from human pluripotent stem cells (hPSCs) by simply adding clickable peptides to the media. It should be widely applicable for elucidating how spatiotemporal changes in morpholog...

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Detalles Bibliográficos
Autores principales: Knight, G. T., Sha, J., Ashton, R. S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Royal Society of Chemistry 2015
Materias:
Chemistry
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456773/
https://www.ncbi.nlm.nih.gov/pubmed/25688384
http://dx.doi.org/10.1039/c4cc08665a
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author Knight, G. T.
Sha, J.
Ashton, R. S.
author_facet Knight, G. T.
Sha, J.
Ashton, R. S.
author_sort Knight, G. T.
collection PubMed
description We describe a modular culture platform that enables spatiotemporal control of the morphology of 2D neural tissues derived from human pluripotent stem cells (hPSCs) by simply adding clickable peptides to the media. It should be widely applicable for elucidating how spatiotemporal changes in morphology and substrate biochemistry regulate tissue morphogenesis.
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spelling pubmed-44567732015-07-08 Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology Knight, G. T. Sha, J. Ashton, R. S. Chem Commun (Camb) Chemistry We describe a modular culture platform that enables spatiotemporal control of the morphology of 2D neural tissues derived from human pluripotent stem cells (hPSCs) by simply adding clickable peptides to the media. It should be widely applicable for elucidating how spatiotemporal changes in morphology and substrate biochemistry regulate tissue morphogenesis. Royal Society of Chemistry 2015-03-28 2015-02-17 /pmc/articles/PMC4456773/ /pubmed/25688384 http://dx.doi.org/10.1039/c4cc08665a Text en This journal is © The Royal Society of Chemistry 2015 http://creativecommons.org/licenses/by/3.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution 3.0 Unported License (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Chemistry
Knight, G. T.
Sha, J.
Ashton, R. S.
Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology
title Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology
title_full Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology
title_fullStr Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology
title_full_unstemmed Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology
title_short Micropatterned, clickable culture substrates enable in situ spatiotemporal control of human PSC-derived neural tissue morphology
title_sort micropatterned, clickable culture substrates enable in situ spatiotemporal control of human psc-derived neural tissue morphology
topic Chemistry
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456773/
https://www.ncbi.nlm.nih.gov/pubmed/25688384
http://dx.doi.org/10.1039/c4cc08665a
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