An effective plasma membrane proteomics approach for small tissue samples

Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective e...

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Autores principales: Smolders, Katrien, Lombaert, Nathalie, Valkenborg, Dirk, Baggerman, Geert, Arckens, Lutgarde
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456939/
https://www.ncbi.nlm.nih.gov/pubmed/26047021
http://dx.doi.org/10.1038/srep10917
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author Smolders, Katrien
Lombaert, Nathalie
Valkenborg, Dirk
Baggerman, Geert
Arckens, Lutgarde
author_facet Smolders, Katrien
Lombaert, Nathalie
Valkenborg, Dirk
Baggerman, Geert
Arckens, Lutgarde
author_sort Smolders, Katrien
collection PubMed
description Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale tissue.
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spelling pubmed-44569392015-06-12 An effective plasma membrane proteomics approach for small tissue samples Smolders, Katrien Lombaert, Nathalie Valkenborg, Dirk Baggerman, Geert Arckens, Lutgarde Sci Rep Article Advancing the quest for new drug targets demands the development of innovative plasma membrane proteome research strategies applicable to small, functionally defined tissue samples. Biotinylation of acute tissue slices and streptavidin pull-down followed by shotgun proteomics allowed the selective extraction and identification of >1,600 proteins of which >60% are associated with the plasma membrane, including (G-protein coupled) receptors, ion channels and transporters, and this from mm(3)-scale tissue. Nature Publishing Group 2015-06-05 /pmc/articles/PMC4456939/ /pubmed/26047021 http://dx.doi.org/10.1038/srep10917 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Smolders, Katrien
Lombaert, Nathalie
Valkenborg, Dirk
Baggerman, Geert
Arckens, Lutgarde
An effective plasma membrane proteomics approach for small tissue samples
title An effective plasma membrane proteomics approach for small tissue samples
title_full An effective plasma membrane proteomics approach for small tissue samples
title_fullStr An effective plasma membrane proteomics approach for small tissue samples
title_full_unstemmed An effective plasma membrane proteomics approach for small tissue samples
title_short An effective plasma membrane proteomics approach for small tissue samples
title_sort effective plasma membrane proteomics approach for small tissue samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4456939/
https://www.ncbi.nlm.nih.gov/pubmed/26047021
http://dx.doi.org/10.1038/srep10917
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