Cargando…

An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin

Heterologous overexpression of functional membrane proteins is a major bottleneck of structural biology. Bacteriorhodopsin from Halobium salinarum (bR) is a striking example of the difficulties in membrane protein overexpression. We suggest a general approach with a finite number of steps which allo...

Descripción completa

Detalles Bibliográficos
Autores principales: Bratanov, Dmitry, Balandin, Taras, Round, Ekaterina, Shevchenko, Vitaly, Gushchin, Ivan, Polovinkin, Vitaly, Borshchevskiy, Valentin, Gordeliy, Valentin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457421/
https://www.ncbi.nlm.nih.gov/pubmed/26046789
http://dx.doi.org/10.1371/journal.pone.0128390