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An Approach to Heterologous Expression of Membrane Proteins. The Case of Bacteriorhodopsin
Heterologous overexpression of functional membrane proteins is a major bottleneck of structural biology. Bacteriorhodopsin from Halobium salinarum (bR) is a striking example of the difficulties in membrane protein overexpression. We suggest a general approach with a finite number of steps which allo...
| Autores principales: | , , , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Public Library of Science
2015
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457421/ https://www.ncbi.nlm.nih.gov/pubmed/26046789 http://dx.doi.org/10.1371/journal.pone.0128390 |