Cargando…

Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets

Previously, we demonstrated that the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) HuN4 strain causes obvious thymic atrophy and thymocytes apoptosis in infected piglets after birth, which is more severe than that induced by classical PRRSV. In this study, we inves...

Descripción completa

Detalles Bibliográficos
Autores principales: Wang, Gang, Yu, Ying, Tu, Yabin, Tong, Jie, Liu, Yonggang, Zhang, Chong, Chang, Yafei, Wang, Shujie, Jiang, Chenggang, Zhou, En-Min, Cai, Xuehui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457848/
https://www.ncbi.nlm.nih.gov/pubmed/26046751
http://dx.doi.org/10.1371/journal.pone.0128292
_version_ 1782375007473631232
author Wang, Gang
Yu, Ying
Tu, Yabin
Tong, Jie
Liu, Yonggang
Zhang, Chong
Chang, Yafei
Wang, Shujie
Jiang, Chenggang
Zhou, En-Min
Cai, Xuehui
author_facet Wang, Gang
Yu, Ying
Tu, Yabin
Tong, Jie
Liu, Yonggang
Zhang, Chong
Chang, Yafei
Wang, Shujie
Jiang, Chenggang
Zhou, En-Min
Cai, Xuehui
author_sort Wang, Gang
collection PubMed
description Previously, we demonstrated that the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) HuN4 strain causes obvious thymic atrophy and thymocytes apoptosis in infected piglets after birth, which is more severe than that induced by classical PRRSV. In this study, we investigated apoptosis and autophagy in the thymus of piglets infected with the HP-PRRSV HuN4 strain, and found that both apoptosis and autophagy occurred in the thymus of piglets infected with HP-PRRSV. In addition to a few virus-infected cells, CD14(+) cells, the main autophagic cells in the thymus were thymic epithelial cells. These findings demonstrated that HP-PRRSV induces apoptosis in bystander cells, and induces autophagy in both infected and bystander cells in the thymus of infected piglets. Herein, we first present new data on the thymic lesions induced by HP-PRRSV, and show that apoptosis and autophagy are key mechanisms involved in cell survival and determinants of the severity of thymic atrophy in infected piglets. Finally, future studies of the mechanism underlying immune responses are proposed based on our current understanding of PRRSV-host interactions.
format Online
Article
Text
id pubmed-4457848
institution National Center for Biotechnology Information
language English
publishDate 2015
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-44578482015-06-09 Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets Wang, Gang Yu, Ying Tu, Yabin Tong, Jie Liu, Yonggang Zhang, Chong Chang, Yafei Wang, Shujie Jiang, Chenggang Zhou, En-Min Cai, Xuehui PLoS One Research Article Previously, we demonstrated that the highly pathogenic porcine reproductive and respiratory syndrome virus (HP-PRRSV) HuN4 strain causes obvious thymic atrophy and thymocytes apoptosis in infected piglets after birth, which is more severe than that induced by classical PRRSV. In this study, we investigated apoptosis and autophagy in the thymus of piglets infected with the HP-PRRSV HuN4 strain, and found that both apoptosis and autophagy occurred in the thymus of piglets infected with HP-PRRSV. In addition to a few virus-infected cells, CD14(+) cells, the main autophagic cells in the thymus were thymic epithelial cells. These findings demonstrated that HP-PRRSV induces apoptosis in bystander cells, and induces autophagy in both infected and bystander cells in the thymus of infected piglets. Herein, we first present new data on the thymic lesions induced by HP-PRRSV, and show that apoptosis and autophagy are key mechanisms involved in cell survival and determinants of the severity of thymic atrophy in infected piglets. Finally, future studies of the mechanism underlying immune responses are proposed based on our current understanding of PRRSV-host interactions. Public Library of Science 2015-06-05 /pmc/articles/PMC4457848/ /pubmed/26046751 http://dx.doi.org/10.1371/journal.pone.0128292 Text en © 2015 Wang et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Wang, Gang
Yu, Ying
Tu, Yabin
Tong, Jie
Liu, Yonggang
Zhang, Chong
Chang, Yafei
Wang, Shujie
Jiang, Chenggang
Zhou, En-Min
Cai, Xuehui
Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets
title Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets
title_full Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets
title_fullStr Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets
title_full_unstemmed Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets
title_short Highly Pathogenic Porcine Reproductive and Respiratory Syndrome Virus Infection Induced Apoptosis and Autophagy in Thymi of Infected Piglets
title_sort highly pathogenic porcine reproductive and respiratory syndrome virus infection induced apoptosis and autophagy in thymi of infected piglets
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4457848/
https://www.ncbi.nlm.nih.gov/pubmed/26046751
http://dx.doi.org/10.1371/journal.pone.0128292
work_keys_str_mv AT wanggang highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT yuying highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT tuyabin highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT tongjie highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT liuyonggang highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT zhangchong highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT changyafei highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT wangshujie highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT jiangchenggang highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT zhouenmin highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets
AT caixuehui highlypathogenicporcinereproductiveandrespiratorysyndromevirusinfectioninducedapoptosisandautophagyinthymiofinfectedpiglets