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Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy

BACKGROUND: Heliotropium strigosum is used in traditional medicine to manage gastrointestinal pain, respiratory distress and vascular disorders. The present study was undertaken to provide scientific evidences for these folkloric uses by in vitro experimental settings. METHODS: A crude methanol extr...

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Autores principales: Janbaz, Khalid H., Javed, Sana, Saqib, Fatima, Imran, Imran, Zia-Ul-Haq, Muhammad, De Feo, Vincenzo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458017/
https://www.ncbi.nlm.nih.gov/pubmed/26048286
http://dx.doi.org/10.1186/s12906-015-0697-1
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author Janbaz, Khalid H.
Javed, Sana
Saqib, Fatima
Imran, Imran
Zia-Ul-Haq, Muhammad
De Feo, Vincenzo
author_facet Janbaz, Khalid H.
Javed, Sana
Saqib, Fatima
Imran, Imran
Zia-Ul-Haq, Muhammad
De Feo, Vincenzo
author_sort Janbaz, Khalid H.
collection PubMed
description BACKGROUND: Heliotropium strigosum is used in traditional medicine to manage gastrointestinal pain, respiratory distress and vascular disorders. The present study was undertaken to provide scientific evidences for these folkloric uses by in vitro experimental settings. METHODS: A crude methanol extract of the Heliotropium strigosum (Hs.Cr) was tested in vitro on isolated rabbit jejunum preparations to detect the possible presence of spasmolytic activity. Moreover, isolated rabbit tracheal and aorta preparations were used to ascertain the relaxant effects of the extract. RESULTS: The Hs.Cr exhibited relaxant effects in rabbit jejunum in a concentration dependent manner (0.01-3.0 mg/ml). The Hs.Cr also relaxed K(+) (80 mM)-induced spastic contractions in rabbit jejunum and shifted the Ca(2+) concentration response curves towards right. The extract relaxed carbachol (1 μM)- as well as K(+) (80 mM)-induced contractions in rabbit trachea at concentrations ranging from 0.01 to 10 mg/ml. Moreover, Hs.Cr. also relaxed (0.01-3.0 mg/ml) the phenylephrine (1 μM)- and K(+) (80 mM)-induced contractions in isolated rabbit aorta. CONCLUSIONS: The Hs.Cr was found to exhibit spasmolytic, bronchodilator and vasorelaxant activities on isolated rabbit jejunum, trachea and aorta preparations, likely mediated through Ca(2+) channel blockade. This finding may provide a scientific basis for the folkloric uses of the plant.
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spelling pubmed-44580172015-06-07 Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy Janbaz, Khalid H. Javed, Sana Saqib, Fatima Imran, Imran Zia-Ul-Haq, Muhammad De Feo, Vincenzo BMC Complement Altern Med Research Article BACKGROUND: Heliotropium strigosum is used in traditional medicine to manage gastrointestinal pain, respiratory distress and vascular disorders. The present study was undertaken to provide scientific evidences for these folkloric uses by in vitro experimental settings. METHODS: A crude methanol extract of the Heliotropium strigosum (Hs.Cr) was tested in vitro on isolated rabbit jejunum preparations to detect the possible presence of spasmolytic activity. Moreover, isolated rabbit tracheal and aorta preparations were used to ascertain the relaxant effects of the extract. RESULTS: The Hs.Cr exhibited relaxant effects in rabbit jejunum in a concentration dependent manner (0.01-3.0 mg/ml). The Hs.Cr also relaxed K(+) (80 mM)-induced spastic contractions in rabbit jejunum and shifted the Ca(2+) concentration response curves towards right. The extract relaxed carbachol (1 μM)- as well as K(+) (80 mM)-induced contractions in rabbit trachea at concentrations ranging from 0.01 to 10 mg/ml. Moreover, Hs.Cr. also relaxed (0.01-3.0 mg/ml) the phenylephrine (1 μM)- and K(+) (80 mM)-induced contractions in isolated rabbit aorta. CONCLUSIONS: The Hs.Cr was found to exhibit spasmolytic, bronchodilator and vasorelaxant activities on isolated rabbit jejunum, trachea and aorta preparations, likely mediated through Ca(2+) channel blockade. This finding may provide a scientific basis for the folkloric uses of the plant. BioMed Central 2015-06-06 /pmc/articles/PMC4458017/ /pubmed/26048286 http://dx.doi.org/10.1186/s12906-015-0697-1 Text en © Janbaz et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Janbaz, Khalid H.
Javed, Sana
Saqib, Fatima
Imran, Imran
Zia-Ul-Haq, Muhammad
De Feo, Vincenzo
Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy
title Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy
title_full Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy
title_fullStr Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy
title_full_unstemmed Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy
title_short Validation of ethnopharmacological uses of Heliotropium strigosum Willd. as spasmolytic, bronchodilator and vasorelaxant remedy
title_sort validation of ethnopharmacological uses of heliotropium strigosum willd. as spasmolytic, bronchodilator and vasorelaxant remedy
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458017/
https://www.ncbi.nlm.nih.gov/pubmed/26048286
http://dx.doi.org/10.1186/s12906-015-0697-1
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