Development of molecular markers for genetic male sterility in Gossypium hirsutum

Genetic male sterility (GMS) in cotton mediated by two homozygous recessive genes, ms5ms5 and ms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. Sequence analysis on ms5 and ms6 loci in Gossypium hirsutum was conducted to reveal genomic variation at these two loci between GMS...

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Autores principales: Feng, Xuehui, Keim, Don, Wanjugi, Humphrey, Coulibaly, Issa, Fu, Yan, Schwarz, John, Huesgen, Scott, Cho, Seungho
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Netherlands 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458262/
https://www.ncbi.nlm.nih.gov/pubmed/26074724
http://dx.doi.org/10.1007/s11032-015-0336-z
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author Feng, Xuehui
Keim, Don
Wanjugi, Humphrey
Coulibaly, Issa
Fu, Yan
Schwarz, John
Huesgen, Scott
Cho, Seungho
author_facet Feng, Xuehui
Keim, Don
Wanjugi, Humphrey
Coulibaly, Issa
Fu, Yan
Schwarz, John
Huesgen, Scott
Cho, Seungho
author_sort Feng, Xuehui
collection PubMed
description Genetic male sterility (GMS) in cotton mediated by two homozygous recessive genes, ms5ms5 and ms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. Sequence analysis on ms5 and ms6 loci in Gossypium hirsutum was conducted to reveal genomic variation at these two loci between GMS and wild-type G. hirsutum inbred lines, and sequence polymorphism linked to ms5 on A12 and ms6 on D12 was revealed. A haplotype marker set that consisted of four SNPs targeting both ms5 and ms6 gene regions was developed and validated for association with GMS in cotton. Predictability of GMS phenotype by this haplotype SNP set was over 99 %. GMS haplotype marker set can serve as a high-throughput molecular breeding tool to select GMS individuals and improve hybrid production efficiency.
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spelling pubmed-44582622015-06-11 Development of molecular markers for genetic male sterility in Gossypium hirsutum Feng, Xuehui Keim, Don Wanjugi, Humphrey Coulibaly, Issa Fu, Yan Schwarz, John Huesgen, Scott Cho, Seungho Mol Breed Article Genetic male sterility (GMS) in cotton mediated by two homozygous recessive genes, ms5ms5 and ms6ms6, is expressed as non-dehiscent anthers and unviable pollen grains. Sequence analysis on ms5 and ms6 loci in Gossypium hirsutum was conducted to reveal genomic variation at these two loci between GMS and wild-type G. hirsutum inbred lines, and sequence polymorphism linked to ms5 on A12 and ms6 on D12 was revealed. A haplotype marker set that consisted of four SNPs targeting both ms5 and ms6 gene regions was developed and validated for association with GMS in cotton. Predictability of GMS phenotype by this haplotype SNP set was over 99 %. GMS haplotype marker set can serve as a high-throughput molecular breeding tool to select GMS individuals and improve hybrid production efficiency. Springer Netherlands 2015-06-07 2015 /pmc/articles/PMC4458262/ /pubmed/26074724 http://dx.doi.org/10.1007/s11032-015-0336-z Text en © The Author(s) 2015 Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
spellingShingle Article
Feng, Xuehui
Keim, Don
Wanjugi, Humphrey
Coulibaly, Issa
Fu, Yan
Schwarz, John
Huesgen, Scott
Cho, Seungho
Development of molecular markers for genetic male sterility in Gossypium hirsutum
title Development of molecular markers for genetic male sterility in Gossypium hirsutum
title_full Development of molecular markers for genetic male sterility in Gossypium hirsutum
title_fullStr Development of molecular markers for genetic male sterility in Gossypium hirsutum
title_full_unstemmed Development of molecular markers for genetic male sterility in Gossypium hirsutum
title_short Development of molecular markers for genetic male sterility in Gossypium hirsutum
title_sort development of molecular markers for genetic male sterility in gossypium hirsutum
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458262/
https://www.ncbi.nlm.nih.gov/pubmed/26074724
http://dx.doi.org/10.1007/s11032-015-0336-z
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