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Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma

BACKGROUND: Lung adenocarcinoma (LAC), the primary histological type of non-small cell lung cancer (NSCLC), has displayed an increasing incidence and mortality worldwide. However, therapeutic approaches were limited. Dysregulation of some lncRNAs has been shown in various types of cancers including...

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Autores principales: Li, Juan, Li, Ping, Zhao, Wei, Yang, Rui, Chen, Shanshan, Bai, Yong, Dun, Shaozhi, Chen, Xiaonan, Du, Yuwen, Wang, Yuanyuan, Zang, Wenqiao, Zhao, Guoqiang, Zhang, Guojun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458341/
https://www.ncbi.nlm.nih.gov/pubmed/26052251
http://dx.doi.org/10.1186/s12935-015-0201-5
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author Li, Juan
Li, Ping
Zhao, Wei
Yang, Rui
Chen, Shanshan
Bai, Yong
Dun, Shaozhi
Chen, Xiaonan
Du, Yuwen
Wang, Yuanyuan
Zang, Wenqiao
Zhao, Guoqiang
Zhang, Guojun
author_facet Li, Juan
Li, Ping
Zhao, Wei
Yang, Rui
Chen, Shanshan
Bai, Yong
Dun, Shaozhi
Chen, Xiaonan
Du, Yuwen
Wang, Yuanyuan
Zang, Wenqiao
Zhao, Guoqiang
Zhang, Guojun
author_sort Li, Juan
collection PubMed
description BACKGROUND: Lung adenocarcinoma (LAC), the primary histological type of non-small cell lung cancer (NSCLC), has displayed an increasing incidence and mortality worldwide. However, therapeutic approaches were limited. Dysregulation of some lncRNAs has been shown in various types of cancers including LAC. The aim of the present study was to vertify lncRNA DLX6-AS1 expression in LAC. METHODS: Microarray assay revealed expression profile of lncRNAs in LAC. qRT-PCR ( quantitative reverse transcription PCR) was performed to identify lncRNA DLX6-AS1 expression level in 72 paired LAC and adjacent normal lung tissues. qRT-PCR and Western blotting were used to verify that down-regulation lncRNA DLX6-AS1 decreased DLX6 (distal-less homeobox 6) mRNA and protein expression. RESULTS: Microarray analysis identified up-regulation of 272 lncRNAs and down-regulation of 635 lncRNAs in LAC tissues. The expression level of lncRNA DLX6-AS1 in LAC tissues was significantly higher compared to paired adjacent normal lung tissues (P< 0.05). In addition, its expression level was closed correlated with both histological differentiation (P = 0.004) and TNM stage (P = 0.033). qRT-PCR and Western blotting analysis showed that DLX6 mRNA and protein levels were lower in si-LncRNA group than in the NC (negative control) and Blank groups. CONCLUSIONS: Microarray analysis identified that lncRNA DLX6-AS1 was up-regulated in LAC tissues. High DLX6-AS1 expression levels were significantly associated with both histological differentiation and TNM stage. Down-regulation of lncRNA DLX6-AS1 expression decreased the DLX6 mRNA and protein levels.
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spelling pubmed-44583412015-06-08 Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma Li, Juan Li, Ping Zhao, Wei Yang, Rui Chen, Shanshan Bai, Yong Dun, Shaozhi Chen, Xiaonan Du, Yuwen Wang, Yuanyuan Zang, Wenqiao Zhao, Guoqiang Zhang, Guojun Cancer Cell Int Primary Research BACKGROUND: Lung adenocarcinoma (LAC), the primary histological type of non-small cell lung cancer (NSCLC), has displayed an increasing incidence and mortality worldwide. However, therapeutic approaches were limited. Dysregulation of some lncRNAs has been shown in various types of cancers including LAC. The aim of the present study was to vertify lncRNA DLX6-AS1 expression in LAC. METHODS: Microarray assay revealed expression profile of lncRNAs in LAC. qRT-PCR ( quantitative reverse transcription PCR) was performed to identify lncRNA DLX6-AS1 expression level in 72 paired LAC and adjacent normal lung tissues. qRT-PCR and Western blotting were used to verify that down-regulation lncRNA DLX6-AS1 decreased DLX6 (distal-less homeobox 6) mRNA and protein expression. RESULTS: Microarray analysis identified up-regulation of 272 lncRNAs and down-regulation of 635 lncRNAs in LAC tissues. The expression level of lncRNA DLX6-AS1 in LAC tissues was significantly higher compared to paired adjacent normal lung tissues (P< 0.05). In addition, its expression level was closed correlated with both histological differentiation (P = 0.004) and TNM stage (P = 0.033). qRT-PCR and Western blotting analysis showed that DLX6 mRNA and protein levels were lower in si-LncRNA group than in the NC (negative control) and Blank groups. CONCLUSIONS: Microarray analysis identified that lncRNA DLX6-AS1 was up-regulated in LAC tissues. High DLX6-AS1 expression levels were significantly associated with both histological differentiation and TNM stage. Down-regulation of lncRNA DLX6-AS1 expression decreased the DLX6 mRNA and protein levels. BioMed Central 2015-05-02 /pmc/articles/PMC4458341/ /pubmed/26052251 http://dx.doi.org/10.1186/s12935-015-0201-5 Text en © Li et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Primary Research
Li, Juan
Li, Ping
Zhao, Wei
Yang, Rui
Chen, Shanshan
Bai, Yong
Dun, Shaozhi
Chen, Xiaonan
Du, Yuwen
Wang, Yuanyuan
Zang, Wenqiao
Zhao, Guoqiang
Zhang, Guojun
Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma
title Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma
title_full Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma
title_fullStr Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma
title_full_unstemmed Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma
title_short Expression of long non-coding RNA DLX6-AS1 in lung adenocarcinoma
title_sort expression of long non-coding rna dlx6-as1 in lung adenocarcinoma
topic Primary Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458341/
https://www.ncbi.nlm.nih.gov/pubmed/26052251
http://dx.doi.org/10.1186/s12935-015-0201-5
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