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Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
BACKGROUND: Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human. OBJECTIVES: The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a pote...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Kowsar
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458350/ https://www.ncbi.nlm.nih.gov/pubmed/26060564 http://dx.doi.org/10.5812/jjm.8(5)2015.17802 |
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author | Abdollahi, Seyed Hossein Ayoobi, Fateme Khorramdelazad, Hossein Nasiri Ahmadabadi, Behzad Rezayati, Mohammadtaghi Kazemi Arababadi, Mohammad Zare-Bidaki, Mohammad |
author_facet | Abdollahi, Seyed Hossein Ayoobi, Fateme Khorramdelazad, Hossein Nasiri Ahmadabadi, Behzad Rezayati, Mohammadtaghi Kazemi Arababadi, Mohammad Zare-Bidaki, Mohammad |
author_sort | Abdollahi, Seyed Hossein |
collection | PubMed |
description | BACKGROUND: Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human. OBJECTIVES: The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a potential candidate for immunization against the parasite and its effect on the production of transforming growth factor-beta (TGF-β). MATERIALS AND METHODS: Toxoplasma gondii tachyzoites were inoculated in the peritoneal cavity of mice and E/SA was harvested and used in animal immunization with and without adjuvant. Serum levels of anti-E/SA antibodies and TGF-β were measured in days 0, 3, 7, 14, 28 and 56 after immunization using ELISA technique. The measurements were statistically analyzed. RESULTS: Our results showed that the serum levels of anti-E/SA immunoglobulins significantly increased in all of the immunized groups. The differences of the serum levels of TGF-β between the groups were statistically significant at days 28 and 56 after immunization with E/SA. CONCLUSIONS: Based on our study, in vivo prepared E/SA may be considered as a good candidate for animal immunization. |
format | Online Article Text |
id | pubmed-4458350 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Kowsar |
record_format | MEDLINE/PubMed |
spelling | pubmed-44583502015-06-09 Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins Abdollahi, Seyed Hossein Ayoobi, Fateme Khorramdelazad, Hossein Nasiri Ahmadabadi, Behzad Rezayati, Mohammadtaghi Kazemi Arababadi, Mohammad Zare-Bidaki, Mohammad Jundishapur J Microbiol Research Article BACKGROUND: Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human. OBJECTIVES: The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a potential candidate for immunization against the parasite and its effect on the production of transforming growth factor-beta (TGF-β). MATERIALS AND METHODS: Toxoplasma gondii tachyzoites were inoculated in the peritoneal cavity of mice and E/SA was harvested and used in animal immunization with and without adjuvant. Serum levels of anti-E/SA antibodies and TGF-β were measured in days 0, 3, 7, 14, 28 and 56 after immunization using ELISA technique. The measurements were statistically analyzed. RESULTS: Our results showed that the serum levels of anti-E/SA immunoglobulins significantly increased in all of the immunized groups. The differences of the serum levels of TGF-β between the groups were statistically significant at days 28 and 56 after immunization with E/SA. CONCLUSIONS: Based on our study, in vivo prepared E/SA may be considered as a good candidate for animal immunization. Kowsar 2015-05-31 /pmc/articles/PMC4458350/ /pubmed/26060564 http://dx.doi.org/10.5812/jjm.8(5)2015.17802 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited. |
spellingShingle | Research Article Abdollahi, Seyed Hossein Ayoobi, Fateme Khorramdelazad, Hossein Nasiri Ahmadabadi, Behzad Rezayati, Mohammadtaghi Kazemi Arababadi, Mohammad Zare-Bidaki, Mohammad Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins |
title | Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins |
title_full | Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins |
title_fullStr | Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins |
title_full_unstemmed | Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins |
title_short | Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins |
title_sort | levels of transforming growth factor-beta after immunization of mice with in vivo prepared toxoplasma gondii excretory/secretory proteins |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458350/ https://www.ncbi.nlm.nih.gov/pubmed/26060564 http://dx.doi.org/10.5812/jjm.8(5)2015.17802 |
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