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Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins

BACKGROUND: Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human. OBJECTIVES: The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a pote...

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Autores principales: Abdollahi, Seyed Hossein, Ayoobi, Fateme, Khorramdelazad, Hossein, Nasiri Ahmadabadi, Behzad, Rezayati, Mohammadtaghi, Kazemi Arababadi, Mohammad, Zare-Bidaki, Mohammad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Kowsar 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458350/
https://www.ncbi.nlm.nih.gov/pubmed/26060564
http://dx.doi.org/10.5812/jjm.8(5)2015.17802
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author Abdollahi, Seyed Hossein
Ayoobi, Fateme
Khorramdelazad, Hossein
Nasiri Ahmadabadi, Behzad
Rezayati, Mohammadtaghi
Kazemi Arababadi, Mohammad
Zare-Bidaki, Mohammad
author_facet Abdollahi, Seyed Hossein
Ayoobi, Fateme
Khorramdelazad, Hossein
Nasiri Ahmadabadi, Behzad
Rezayati, Mohammadtaghi
Kazemi Arababadi, Mohammad
Zare-Bidaki, Mohammad
author_sort Abdollahi, Seyed Hossein
collection PubMed
description BACKGROUND: Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human. OBJECTIVES: The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a potential candidate for immunization against the parasite and its effect on the production of transforming growth factor-beta (TGF-β). MATERIALS AND METHODS: Toxoplasma gondii tachyzoites were inoculated in the peritoneal cavity of mice and E/SA was harvested and used in animal immunization with and without adjuvant. Serum levels of anti-E/SA antibodies and TGF-β were measured in days 0, 3, 7, 14, 28 and 56 after immunization using ELISA technique. The measurements were statistically analyzed. RESULTS: Our results showed that the serum levels of anti-E/SA immunoglobulins significantly increased in all of the immunized groups. The differences of the serum levels of TGF-β between the groups were statistically significant at days 28 and 56 after immunization with E/SA. CONCLUSIONS: Based on our study, in vivo prepared E/SA may be considered as a good candidate for animal immunization.
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spelling pubmed-44583502015-06-09 Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins Abdollahi, Seyed Hossein Ayoobi, Fateme Khorramdelazad, Hossein Nasiri Ahmadabadi, Behzad Rezayati, Mohammadtaghi Kazemi Arababadi, Mohammad Zare-Bidaki, Mohammad Jundishapur J Microbiol Research Article BACKGROUND: Zoonotic parasite Toxoplasma gondii has a high prevalence in human populations. A suitable vaccine for animals can stop the transmission of infection between animal and human. OBJECTIVES: The aim of this study was to evaluate in vivo prepared excretory/secretory antigens (E/SA) as a potential candidate for immunization against the parasite and its effect on the production of transforming growth factor-beta (TGF-β). MATERIALS AND METHODS: Toxoplasma gondii tachyzoites were inoculated in the peritoneal cavity of mice and E/SA was harvested and used in animal immunization with and without adjuvant. Serum levels of anti-E/SA antibodies and TGF-β were measured in days 0, 3, 7, 14, 28 and 56 after immunization using ELISA technique. The measurements were statistically analyzed. RESULTS: Our results showed that the serum levels of anti-E/SA immunoglobulins significantly increased in all of the immunized groups. The differences of the serum levels of TGF-β between the groups were statistically significant at days 28 and 56 after immunization with E/SA. CONCLUSIONS: Based on our study, in vivo prepared E/SA may be considered as a good candidate for animal immunization. Kowsar 2015-05-31 /pmc/articles/PMC4458350/ /pubmed/26060564 http://dx.doi.org/10.5812/jjm.8(5)2015.17802 Text en Copyright © 2015, Ahvaz Jundishapur University of Medical Sciences. http://creativecommons.org/licenses/by-nc/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial 4.0 International License (http://creativecommons.org/licenses/by-nc/4.0/) which permits copy and redistribute the material just in noncommercial usages, provided the original work is properly cited.
spellingShingle Research Article
Abdollahi, Seyed Hossein
Ayoobi, Fateme
Khorramdelazad, Hossein
Nasiri Ahmadabadi, Behzad
Rezayati, Mohammadtaghi
Kazemi Arababadi, Mohammad
Zare-Bidaki, Mohammad
Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
title Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
title_full Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
title_fullStr Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
title_full_unstemmed Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
title_short Levels of Transforming Growth Factor-Beta After Immunization of Mice With in vivo prepared Toxoplasma gondii Excretory/Secretory Proteins
title_sort levels of transforming growth factor-beta after immunization of mice with in vivo prepared toxoplasma gondii excretory/secretory proteins
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458350/
https://www.ncbi.nlm.nih.gov/pubmed/26060564
http://dx.doi.org/10.5812/jjm.8(5)2015.17802
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