Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters

We present an attolitre-sized arrayed lipid bilayer chamber system (aL-ALBiC) for rapid and massively parallel single-molecule assay of membrane transporter activity. Because of the small reaction volume (200 aL), the aL-ALBiC performed fast detection of single transporter activity, thereby enhancin...

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Detalles Bibliográficos
Autores principales: Soga, Naoki, Watanabe, Rikiya, Noji, Hiroyuki
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458879/
https://www.ncbi.nlm.nih.gov/pubmed/26052065
http://dx.doi.org/10.1038/srep11025
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author Soga, Naoki
Watanabe, Rikiya
Noji, Hiroyuki
author_facet Soga, Naoki
Watanabe, Rikiya
Noji, Hiroyuki
author_sort Soga, Naoki
collection PubMed
description We present an attolitre-sized arrayed lipid bilayer chamber system (aL-ALBiC) for rapid and massively parallel single-molecule assay of membrane transporter activity. Because of the small reaction volume (200 aL), the aL-ALBiC performed fast detection of single transporter activity, thereby enhancing the sensitivity, throughput, and accuracy of the analysis. Thus, aL-ALBiC broadens the opportunities for single-molecule analysis of various membrane transporters and can be used in pharmaceutical applications such as drug screening.
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spelling pubmed-44588792015-06-17 Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters Soga, Naoki Watanabe, Rikiya Noji, Hiroyuki Sci Rep Article We present an attolitre-sized arrayed lipid bilayer chamber system (aL-ALBiC) for rapid and massively parallel single-molecule assay of membrane transporter activity. Because of the small reaction volume (200 aL), the aL-ALBiC performed fast detection of single transporter activity, thereby enhancing the sensitivity, throughput, and accuracy of the analysis. Thus, aL-ALBiC broadens the opportunities for single-molecule analysis of various membrane transporters and can be used in pharmaceutical applications such as drug screening. Nature Publishing Group 2015-06-08 /pmc/articles/PMC4458879/ /pubmed/26052065 http://dx.doi.org/10.1038/srep11025 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Soga, Naoki
Watanabe, Rikiya
Noji, Hiroyuki
Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
title Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
title_full Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
title_fullStr Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
title_full_unstemmed Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
title_short Attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
title_sort attolitre-sized lipid bilayer chamber array for rapid detection of single transporters
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4458879/
https://www.ncbi.nlm.nih.gov/pubmed/26052065
http://dx.doi.org/10.1038/srep11025
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AT nojihiroyuki attolitresizedlipidbilayerchamberarrayforrapiddetectionofsingletransporters

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