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Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state

OBJECTIVE: This study aimed to evaluate the influence of fetal hemoglobin (Hb F) on hemolysis biomarkers in sickle cell anemia patients. METHODS: Fifty adult sickle cell anemia patients were included in the study. All patients were taking hydroxyurea for at least six months and were followed at the...

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Autores principales: Moreira, Juliane Almeida, Laurentino, Marília Rocha, Machado, Rosângela Pinheiro Gonçalves, Barbosa, Maritza Cavalcante, Gonçalves, Ronaldo Pinheiro, Mota, Amanda de Menezes, Rocha, Lilianne Brito da Silva, Martins, Alice Maria Costa, de Lima Arruda, Alcínia Braga, de Souza, Iêda Pereira, Gonçalves, Romélia Pinheiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Sociedade Brasileira de Hematologia e Hemoterapia 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459481/
https://www.ncbi.nlm.nih.gov/pubmed/26041418
http://dx.doi.org/10.1016/j.bjhh.2015.01.008
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author Moreira, Juliane Almeida
Laurentino, Marília Rocha
Machado, Rosângela Pinheiro Gonçalves
Barbosa, Maritza Cavalcante
Gonçalves, Ronaldo Pinheiro
Mota, Amanda de Menezes
Rocha, Lilianne Brito da Silva
Martins, Alice Maria Costa
de Lima Arruda, Alcínia Braga
de Souza, Iêda Pereira
Gonçalves, Romélia Pinheiro
author_facet Moreira, Juliane Almeida
Laurentino, Marília Rocha
Machado, Rosângela Pinheiro Gonçalves
Barbosa, Maritza Cavalcante
Gonçalves, Ronaldo Pinheiro
Mota, Amanda de Menezes
Rocha, Lilianne Brito da Silva
Martins, Alice Maria Costa
de Lima Arruda, Alcínia Braga
de Souza, Iêda Pereira
Gonçalves, Romélia Pinheiro
author_sort Moreira, Juliane Almeida
collection PubMed
description OBJECTIVE: This study aimed to evaluate the influence of fetal hemoglobin (Hb F) on hemolysis biomarkers in sickle cell anemia patients. METHODS: Fifty adult sickle cell anemia patients were included in the study. All patients were taking hydroxyurea for at least six months and were followed at the outpatient clinic of a hospital in Fortaleza, Ceará, Brazil. The control group consisted of 20 hemoglobin AA individuals. The reticulocyte count was performed by an automated methodology, lactate dehydrogenase and uric acid were measured by spectrophotometry and arginase I by enzyme-linked immunosorbent assay (ELISA). The presence of Hb S was detected by high-performance liquid chromatography. The level of significance was set for a p-value <0.05. RESULTS: A significant increase was observed in the reticulocyte count and lactate dehydrogenase, uric acid and arginase I levels in sickle cell anemia patients compared to the control group (p-value <0.05). Patients having Hb F levels greater than 10% showed a significant decrease in the reticulocyte count, arginase I and lactate dehydrogenase. A significant decrease was observed in arginase I levels in patients taking hydroxyurea at a dose greater than 20 mg/kg/day. CONCLUSION: The results of this study show that sickle cell anemia patients have increases in the hemolysis biomarkers, lactate dehydrogenase, reticulocyte count, arginase I, uric acid and increases in Hb F can reduce the reticulocyte count and arginase I and lactate dehydrogenase levels.
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spelling pubmed-44594812015-06-16 Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state Moreira, Juliane Almeida Laurentino, Marília Rocha Machado, Rosângela Pinheiro Gonçalves Barbosa, Maritza Cavalcante Gonçalves, Ronaldo Pinheiro Mota, Amanda de Menezes Rocha, Lilianne Brito da Silva Martins, Alice Maria Costa de Lima Arruda, Alcínia Braga de Souza, Iêda Pereira Gonçalves, Romélia Pinheiro Rev Bras Hematol Hemoter Original Article OBJECTIVE: This study aimed to evaluate the influence of fetal hemoglobin (Hb F) on hemolysis biomarkers in sickle cell anemia patients. METHODS: Fifty adult sickle cell anemia patients were included in the study. All patients were taking hydroxyurea for at least six months and were followed at the outpatient clinic of a hospital in Fortaleza, Ceará, Brazil. The control group consisted of 20 hemoglobin AA individuals. The reticulocyte count was performed by an automated methodology, lactate dehydrogenase and uric acid were measured by spectrophotometry and arginase I by enzyme-linked immunosorbent assay (ELISA). The presence of Hb S was detected by high-performance liquid chromatography. The level of significance was set for a p-value <0.05. RESULTS: A significant increase was observed in the reticulocyte count and lactate dehydrogenase, uric acid and arginase I levels in sickle cell anemia patients compared to the control group (p-value <0.05). Patients having Hb F levels greater than 10% showed a significant decrease in the reticulocyte count, arginase I and lactate dehydrogenase. A significant decrease was observed in arginase I levels in patients taking hydroxyurea at a dose greater than 20 mg/kg/day. CONCLUSION: The results of this study show that sickle cell anemia patients have increases in the hemolysis biomarkers, lactate dehydrogenase, reticulocyte count, arginase I, uric acid and increases in Hb F can reduce the reticulocyte count and arginase I and lactate dehydrogenase levels. Sociedade Brasileira de Hematologia e Hemoterapia 2015 2015-04-14 /pmc/articles/PMC4459481/ /pubmed/26041418 http://dx.doi.org/10.1016/j.bjhh.2015.01.008 Text en © 2015 Associação Brasileira de Hematologia, Hemoterapia e Terapia Celular. Published by Elsevier Editora Ltda. All rights reserved. http://creativecommons.org/licenses/by-nc-nd/4.0/ This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Original Article
Moreira, Juliane Almeida
Laurentino, Marília Rocha
Machado, Rosângela Pinheiro Gonçalves
Barbosa, Maritza Cavalcante
Gonçalves, Ronaldo Pinheiro
Mota, Amanda de Menezes
Rocha, Lilianne Brito da Silva
Martins, Alice Maria Costa
de Lima Arruda, Alcínia Braga
de Souza, Iêda Pereira
Gonçalves, Romélia Pinheiro
Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state
title Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state
title_full Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state
title_fullStr Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state
title_full_unstemmed Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state
title_short Pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state
title_sort pattern of hemolysis parameters and association with fetal hemoglobin in sickle cell anemia patients in steady state
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459481/
https://www.ncbi.nlm.nih.gov/pubmed/26041418
http://dx.doi.org/10.1016/j.bjhh.2015.01.008
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