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Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction

BACKGROUND: Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as...

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Autores principales: Li, Chencheng, Chen, Xiaonan, Huang, Junwen, Sun, Qianqian, Wang, Lei
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459687/
https://www.ncbi.nlm.nih.gov/pubmed/26044724
http://dx.doi.org/10.1186/s40001-015-0148-y
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author Li, Chencheng
Chen, Xiaonan
Huang, Junwen
Sun, Qianqian
Wang, Lei
author_facet Li, Chencheng
Chen, Xiaonan
Huang, Junwen
Sun, Qianqian
Wang, Lei
author_sort Li, Chencheng
collection PubMed
description BACKGROUND: Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as indicators for AMI. METHODS: Detection of AMI patients and normal persons by using miRNA microarray chip analysis and miR-26a, miR-191, and miR-208b was screened out. Eighty-seven AMI patients and eighty-seven homogeneous healthy individuals were recruited. Total mRNA including miRNA was isolated and miR-26a, miR-191, and miR-208b expression were determined by qRT-PCR. Receiver operating characteristic curve analysis was performed to evaluate the instructive power of miR-26a, miR-191, and miR-208b for AMI. Dual-luciferase reporter assays indicated p21 is a direct target of miR-208b. RESULTS: miR-26a and miR-191 were low expressed in AMI compared with normal healthy people, but miR-208b was expressed at a high level in AMI. miR-26a showed an area under the curve (AUC) of 0.745, with a sensitivity of 73.6 % and a specificity of 72.4 %.The AUC for miR-191 was 0.669, with a sensitivity of 62.1 % and a specificity of 69.0 %.The AUC for miR-208b was 0.674, with a sensitivity of 59.8 % and a specificity of 73.6 %. CONCLUSIONS: miR-208b was significantly increased in the AMI compared with healthy people, while miR-26a and miR-191 were decreased. miR-26a, miR-191, and miR-208b were potential indices of AMI, and miR-208b was more effective in patients with non-ST-elevation myocardial infarction.
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spelling pubmed-44596872015-06-09 Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction Li, Chencheng Chen, Xiaonan Huang, Junwen Sun, Qianqian Wang, Lei Eur J Med Res Research BACKGROUND: Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as indicators for AMI. METHODS: Detection of AMI patients and normal persons by using miRNA microarray chip analysis and miR-26a, miR-191, and miR-208b was screened out. Eighty-seven AMI patients and eighty-seven homogeneous healthy individuals were recruited. Total mRNA including miRNA was isolated and miR-26a, miR-191, and miR-208b expression were determined by qRT-PCR. Receiver operating characteristic curve analysis was performed to evaluate the instructive power of miR-26a, miR-191, and miR-208b for AMI. Dual-luciferase reporter assays indicated p21 is a direct target of miR-208b. RESULTS: miR-26a and miR-191 were low expressed in AMI compared with normal healthy people, but miR-208b was expressed at a high level in AMI. miR-26a showed an area under the curve (AUC) of 0.745, with a sensitivity of 73.6 % and a specificity of 72.4 %.The AUC for miR-191 was 0.669, with a sensitivity of 62.1 % and a specificity of 69.0 %.The AUC for miR-208b was 0.674, with a sensitivity of 59.8 % and a specificity of 73.6 %. CONCLUSIONS: miR-208b was significantly increased in the AMI compared with healthy people, while miR-26a and miR-191 were decreased. miR-26a, miR-191, and miR-208b were potential indices of AMI, and miR-208b was more effective in patients with non-ST-elevation myocardial infarction. BioMed Central 2015-06-05 /pmc/articles/PMC4459687/ /pubmed/26044724 http://dx.doi.org/10.1186/s40001-015-0148-y Text en © Li et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Li, Chencheng
Chen, Xiaonan
Huang, Junwen
Sun, Qianqian
Wang, Lei
Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction
title Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction
title_full Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction
title_fullStr Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction
title_full_unstemmed Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction
title_short Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction
title_sort clinical impact of circulating mir-26a, mir-191, and mir-208b in plasma of patients with acute myocardial infarction
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459687/
https://www.ncbi.nlm.nih.gov/pubmed/26044724
http://dx.doi.org/10.1186/s40001-015-0148-y
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