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Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction
BACKGROUND: Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459687/ https://www.ncbi.nlm.nih.gov/pubmed/26044724 http://dx.doi.org/10.1186/s40001-015-0148-y |
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author | Li, Chencheng Chen, Xiaonan Huang, Junwen Sun, Qianqian Wang, Lei |
author_facet | Li, Chencheng Chen, Xiaonan Huang, Junwen Sun, Qianqian Wang, Lei |
author_sort | Li, Chencheng |
collection | PubMed |
description | BACKGROUND: Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as indicators for AMI. METHODS: Detection of AMI patients and normal persons by using miRNA microarray chip analysis and miR-26a, miR-191, and miR-208b was screened out. Eighty-seven AMI patients and eighty-seven homogeneous healthy individuals were recruited. Total mRNA including miRNA was isolated and miR-26a, miR-191, and miR-208b expression were determined by qRT-PCR. Receiver operating characteristic curve analysis was performed to evaluate the instructive power of miR-26a, miR-191, and miR-208b for AMI. Dual-luciferase reporter assays indicated p21 is a direct target of miR-208b. RESULTS: miR-26a and miR-191 were low expressed in AMI compared with normal healthy people, but miR-208b was expressed at a high level in AMI. miR-26a showed an area under the curve (AUC) of 0.745, with a sensitivity of 73.6 % and a specificity of 72.4 %.The AUC for miR-191 was 0.669, with a sensitivity of 62.1 % and a specificity of 69.0 %.The AUC for miR-208b was 0.674, with a sensitivity of 59.8 % and a specificity of 73.6 %. CONCLUSIONS: miR-208b was significantly increased in the AMI compared with healthy people, while miR-26a and miR-191 were decreased. miR-26a, miR-191, and miR-208b were potential indices of AMI, and miR-208b was more effective in patients with non-ST-elevation myocardial infarction. |
format | Online Article Text |
id | pubmed-4459687 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44596872015-06-09 Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction Li, Chencheng Chen, Xiaonan Huang, Junwen Sun, Qianqian Wang, Lei Eur J Med Res Research BACKGROUND: Aberrant expression of several types of miRNAs has been reported in acute myocardial infarction (AMI). The objective of our study was to compare miRNA expression in AMI patients and normal healthy people and determine whether miR-26a, miR-191, and miR-208b could be measured in plasma as indicators for AMI. METHODS: Detection of AMI patients and normal persons by using miRNA microarray chip analysis and miR-26a, miR-191, and miR-208b was screened out. Eighty-seven AMI patients and eighty-seven homogeneous healthy individuals were recruited. Total mRNA including miRNA was isolated and miR-26a, miR-191, and miR-208b expression were determined by qRT-PCR. Receiver operating characteristic curve analysis was performed to evaluate the instructive power of miR-26a, miR-191, and miR-208b for AMI. Dual-luciferase reporter assays indicated p21 is a direct target of miR-208b. RESULTS: miR-26a and miR-191 were low expressed in AMI compared with normal healthy people, but miR-208b was expressed at a high level in AMI. miR-26a showed an area under the curve (AUC) of 0.745, with a sensitivity of 73.6 % and a specificity of 72.4 %.The AUC for miR-191 was 0.669, with a sensitivity of 62.1 % and a specificity of 69.0 %.The AUC for miR-208b was 0.674, with a sensitivity of 59.8 % and a specificity of 73.6 %. CONCLUSIONS: miR-208b was significantly increased in the AMI compared with healthy people, while miR-26a and miR-191 were decreased. miR-26a, miR-191, and miR-208b were potential indices of AMI, and miR-208b was more effective in patients with non-ST-elevation myocardial infarction. BioMed Central 2015-06-05 /pmc/articles/PMC4459687/ /pubmed/26044724 http://dx.doi.org/10.1186/s40001-015-0148-y Text en © Li et al. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Li, Chencheng Chen, Xiaonan Huang, Junwen Sun, Qianqian Wang, Lei Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction |
title | Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction |
title_full | Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction |
title_fullStr | Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction |
title_full_unstemmed | Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction |
title_short | Clinical impact of circulating miR-26a, miR-191, and miR-208b in plasma of patients with acute myocardial infarction |
title_sort | clinical impact of circulating mir-26a, mir-191, and mir-208b in plasma of patients with acute myocardial infarction |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459687/ https://www.ncbi.nlm.nih.gov/pubmed/26044724 http://dx.doi.org/10.1186/s40001-015-0148-y |
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