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Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia

Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400...

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Autores principales: Goes, Elenice S. R., Lara, Jorge A. F., Gasparino, Eliane, Del Vesco, Ana P., Goes, Marcio D., Alexandre Filho, Luiz, Ribeiro, Ricardo P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459807/
https://www.ncbi.nlm.nih.gov/pubmed/26053858
http://dx.doi.org/10.1371/journal.pone.0129145
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author Goes, Elenice S. R.
Lara, Jorge A. F.
Gasparino, Eliane
Del Vesco, Ana P.
Goes, Marcio D.
Alexandre Filho, Luiz
Ribeiro, Ricardo P.
author_facet Goes, Elenice S. R.
Lara, Jorge A. F.
Gasparino, Eliane
Del Vesco, Ana P.
Goes, Marcio D.
Alexandre Filho, Luiz
Ribeiro, Ricardo P.
author_sort Goes, Elenice S. R.
collection PubMed
description Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, and 240 minutes).Transportation times alone reduced cortisol levels up to 180 minutes, followed by increased WHC and mRNA expression, RyR1 and RyR3 (200 kg/m³ density). No effect of density x transportation time interacted on the evaluated parameters. Results provided the first evidence that pre-slaughter stress affected ryanodine gene expression receptors and, consequently, the water-holding capacity in tilapia fillets.
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spelling pubmed-44598072015-06-16 Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia Goes, Elenice S. R. Lara, Jorge A. F. Gasparino, Eliane Del Vesco, Ana P. Goes, Marcio D. Alexandre Filho, Luiz Ribeiro, Ricardo P. PLoS One Research Article Current study evaluated the effect of pre-slaughter stress on serum cortisol levels, pH, colorimetry, water-holding capacity (WHC) and gene expression of ryanodine receptors (RyR1 and RyR3) in the Nile tilapia. A 3x4 factorial scheme experiment was conducted comprising three densities (100, 200, 400 kg/m³) with four transportation times (60, 120, 180, and 240 minutes).Transportation times alone reduced cortisol levels up to 180 minutes, followed by increased WHC and mRNA expression, RyR1 and RyR3 (200 kg/m³ density). No effect of density x transportation time interacted on the evaluated parameters. Results provided the first evidence that pre-slaughter stress affected ryanodine gene expression receptors and, consequently, the water-holding capacity in tilapia fillets. Public Library of Science 2015-06-08 /pmc/articles/PMC4459807/ /pubmed/26053858 http://dx.doi.org/10.1371/journal.pone.0129145 Text en © 2015 Goes et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Goes, Elenice S. R.
Lara, Jorge A. F.
Gasparino, Eliane
Del Vesco, Ana P.
Goes, Marcio D.
Alexandre Filho, Luiz
Ribeiro, Ricardo P.
Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia
title Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia
title_full Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia
title_fullStr Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia
title_full_unstemmed Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia
title_short Pre-Slaughter Stress Affects Ryanodine Receptor Protein Gene Expression and the Water-Holding Capacity in Fillets of the Nile Tilapia
title_sort pre-slaughter stress affects ryanodine receptor protein gene expression and the water-holding capacity in fillets of the nile tilapia
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459807/
https://www.ncbi.nlm.nih.gov/pubmed/26053858
http://dx.doi.org/10.1371/journal.pone.0129145
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