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Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia
Clinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evalua...
Autores principales: | , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459827/ https://www.ncbi.nlm.nih.gov/pubmed/26053038 http://dx.doi.org/10.1371/journal.pone.0128397 |
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author | Iyomasa, Mamie Mizusaki Fernandes, Fernanda Silva Iyomasa, Daniela Mizusaki Pereira, Yamba Carla Lara Fernández, Rodrigo Alberto Restrepo Calzzani, Ricardo Alexandre Nascimento, Glauce Crivelaro Leite-Panissi, Christie Ramos Andrade Issa, João Paulo Mardegan |
author_facet | Iyomasa, Mamie Mizusaki Fernandes, Fernanda Silva Iyomasa, Daniela Mizusaki Pereira, Yamba Carla Lara Fernández, Rodrigo Alberto Restrepo Calzzani, Ricardo Alexandre Nascimento, Glauce Crivelaro Leite-Panissi, Christie Ramos Andrade Issa, João Paulo Mardegan |
author_sort | Iyomasa, Mamie Mizusaki |
collection | PubMed |
description | Clinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evaluate the influence of acute stress on metabolic activity and oxidative stress of masseter muscles of rats subjected to occlusal modification through morphological and histochemical analyses. In this study, adult Wistar rats were divided into 4 groups: a group with extraction and acute stress (E+A); group with extraction and without stress (E+C); group without extraction and with acute stress (NO+A); and control group without both extraction and stress (NO+C). Masseter muscles were analyzed by Succinate Dehydrogenase (SDH), Nicotinamide Adenine Dinucleotide Diaphorase (NADH) and Reactive Oxygen Species (ROS) techniques. Statistical analyses and two-way ANOVA were applied, followed by Tukey-Kramer tests. In the SDH test, the E+C, E+A and NO+A groups showed a decrease in high desidrogenase activities fibers (P < 0.05), compared to the NO+C group. In the NADH test, there was no difference among the different groups. In the ROS test, in contrast, E+A, E+C and NO+A groups showed a decrease in ROS expression, compared to NO+C groups (P < 0.05). Modified dental occlusion and acute stress - which are important and prevalent problems that affect the general population - are important etiologic factors in metabolic plasticity and ROS levels of masseter muscles. |
format | Online Article Text |
id | pubmed-4459827 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44598272015-06-16 Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia Iyomasa, Mamie Mizusaki Fernandes, Fernanda Silva Iyomasa, Daniela Mizusaki Pereira, Yamba Carla Lara Fernández, Rodrigo Alberto Restrepo Calzzani, Ricardo Alexandre Nascimento, Glauce Crivelaro Leite-Panissi, Christie Ramos Andrade Issa, João Paulo Mardegan PLoS One Research Article Clinical evidence has shown that stress may be associated with alterations in masticatory muscle functions. Morphological changes in masticatory muscles induced by occlusal alterations and associated with emotional stress are still lacking in the literature. The objective of this study was to evaluate the influence of acute stress on metabolic activity and oxidative stress of masseter muscles of rats subjected to occlusal modification through morphological and histochemical analyses. In this study, adult Wistar rats were divided into 4 groups: a group with extraction and acute stress (E+A); group with extraction and without stress (E+C); group without extraction and with acute stress (NO+A); and control group without both extraction and stress (NO+C). Masseter muscles were analyzed by Succinate Dehydrogenase (SDH), Nicotinamide Adenine Dinucleotide Diaphorase (NADH) and Reactive Oxygen Species (ROS) techniques. Statistical analyses and two-way ANOVA were applied, followed by Tukey-Kramer tests. In the SDH test, the E+C, E+A and NO+A groups showed a decrease in high desidrogenase activities fibers (P < 0.05), compared to the NO+C group. In the NADH test, there was no difference among the different groups. In the ROS test, in contrast, E+A, E+C and NO+A groups showed a decrease in ROS expression, compared to NO+C groups (P < 0.05). Modified dental occlusion and acute stress - which are important and prevalent problems that affect the general population - are important etiologic factors in metabolic plasticity and ROS levels of masseter muscles. Public Library of Science 2015-06-08 /pmc/articles/PMC4459827/ /pubmed/26053038 http://dx.doi.org/10.1371/journal.pone.0128397 Text en © 2015 Iyomasa et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Iyomasa, Mamie Mizusaki Fernandes, Fernanda Silva Iyomasa, Daniela Mizusaki Pereira, Yamba Carla Lara Fernández, Rodrigo Alberto Restrepo Calzzani, Ricardo Alexandre Nascimento, Glauce Crivelaro Leite-Panissi, Christie Ramos Andrade Issa, João Paulo Mardegan Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia |
title | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia |
title_full | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia |
title_fullStr | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia |
title_full_unstemmed | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia |
title_short | Metabolic Changes in Masseter Muscle of Rats Submitted to Acute Stress Associated with Exodontia |
title_sort | metabolic changes in masseter muscle of rats submitted to acute stress associated with exodontia |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459827/ https://www.ncbi.nlm.nih.gov/pubmed/26053038 http://dx.doi.org/10.1371/journal.pone.0128397 |
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