From hundreds to thousands: Widening the normal human Urinome

The limits on protein detection in urine are unknown. Improving the analytical approach to detection would increase the number of identified proteins and potentially strengthen their predictive potential in diseases. Here, we present the data that resulted from a combination of analytical procedures...

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Detalles Bibliográficos
Autores principales: Santucci, Laura, Candiano, Giovanni, Petretto, Andrea, Bruschi, Maurizio, Lavarello, Chiara, Inglese, Elvira, Giorgio Righetti, Pier, Marco Ghiggeri, Gian
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2014
Materias:
Data Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459867/
https://www.ncbi.nlm.nih.gov/pubmed/26217681
http://dx.doi.org/10.1016/j.dib.2014.08.006
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author Santucci, Laura
Candiano, Giovanni
Petretto, Andrea
Bruschi, Maurizio
Lavarello, Chiara
Inglese, Elvira
Giorgio Righetti, Pier
Marco Ghiggeri, Gian
author_facet Santucci, Laura
Candiano, Giovanni
Petretto, Andrea
Bruschi, Maurizio
Lavarello, Chiara
Inglese, Elvira
Giorgio Righetti, Pier
Marco Ghiggeri, Gian
author_sort Santucci, Laura
collection PubMed
description The limits on protein detection in urine are unknown. Improving the analytical approach to detection would increase the number of identified proteins and potentially strengthen their predictive potential in diseases. Here, we present the data that resulted from a combination of analytical procedures for maximizing sensitivity and reproducibility of normal human urinary proteome analysis. These procedures are ultracentrifugation, vesicle separation, combinatorial peptide ligand libraries (CPLL) and solvent removal of pigments. Proteins were identified by an Orbitrap Velos Mass Spectrometry. 3429 proteins are characterized, 1724 of which are novel discoveries. The data are related to Santucci et al. (in press) [1] and available both here and at ChorusProject.org under project name “From hundreds to thousands: widening the normal human Urinome”. The material supplied to Chorus Progect.org includes technical MS spectra data only.
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spelling pubmed-44598672015-07-27 From hundreds to thousands: Widening the normal human Urinome Santucci, Laura Candiano, Giovanni Petretto, Andrea Bruschi, Maurizio Lavarello, Chiara Inglese, Elvira Giorgio Righetti, Pier Marco Ghiggeri, Gian Data Brief Data Article The limits on protein detection in urine are unknown. Improving the analytical approach to detection would increase the number of identified proteins and potentially strengthen their predictive potential in diseases. Here, we present the data that resulted from a combination of analytical procedures for maximizing sensitivity and reproducibility of normal human urinary proteome analysis. These procedures are ultracentrifugation, vesicle separation, combinatorial peptide ligand libraries (CPLL) and solvent removal of pigments. Proteins were identified by an Orbitrap Velos Mass Spectrometry. 3429 proteins are characterized, 1724 of which are novel discoveries. The data are related to Santucci et al. (in press) [1] and available both here and at ChorusProject.org under project name “From hundreds to thousands: widening the normal human Urinome”. The material supplied to Chorus Progect.org includes technical MS spectra data only. Elsevier 2014-08-22 /pmc/articles/PMC4459867/ /pubmed/26217681 http://dx.doi.org/10.1016/j.dib.2014.08.006 Text en © 2014 The Authors http://creativecommons.org/licenses/by/3.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/3.0/).
spellingShingle Data Article
Santucci, Laura
Candiano, Giovanni
Petretto, Andrea
Bruschi, Maurizio
Lavarello, Chiara
Inglese, Elvira
Giorgio Righetti, Pier
Marco Ghiggeri, Gian
From hundreds to thousands: Widening the normal human Urinome
title From hundreds to thousands: Widening the normal human Urinome
title_full From hundreds to thousands: Widening the normal human Urinome
title_fullStr From hundreds to thousands: Widening the normal human Urinome
title_full_unstemmed From hundreds to thousands: Widening the normal human Urinome
title_short From hundreds to thousands: Widening the normal human Urinome
title_sort from hundreds to thousands: widening the normal human urinome
topic Data Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4459867/
https://www.ncbi.nlm.nih.gov/pubmed/26217681
http://dx.doi.org/10.1016/j.dib.2014.08.006
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