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Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection

Different Leishmania species cause distinct clinical manifestations of the infectious disease leishmaniasis. It is fundamentally important to understand the mechanisms governing the interaction between Leishmania and its host cell. Little is known about this interaction between Leishmania (Viannia)...

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Autores principales: Ovalle-Bracho, Clemencia, Franco-Muñoz, Carlos, Londoño-Barbosa, Diana, Restrepo-Montoya, Daniel, Clavijo-Ramírez, Carlos
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460072/
https://www.ncbi.nlm.nih.gov/pubmed/26052705
http://dx.doi.org/10.1371/journal.pone.0128934
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author Ovalle-Bracho, Clemencia
Franco-Muñoz, Carlos
Londoño-Barbosa, Diana
Restrepo-Montoya, Daniel
Clavijo-Ramírez, Carlos
author_facet Ovalle-Bracho, Clemencia
Franco-Muñoz, Carlos
Londoño-Barbosa, Diana
Restrepo-Montoya, Daniel
Clavijo-Ramírez, Carlos
author_sort Ovalle-Bracho, Clemencia
collection PubMed
description Different Leishmania species cause distinct clinical manifestations of the infectious disease leishmaniasis. It is fundamentally important to understand the mechanisms governing the interaction between Leishmania and its host cell. Little is known about this interaction between Leishmania (Viannia) braziliensis and human macrophages. In this study, we aimed to identify differential gene expression between non-infected and L. (V) braziliensis-infected U937-derived macrophages. We deployed a whole human transcriptome microarray analysis using 72 hours post-infection samples and compared those samples with their non-infected counterparts. We found that 218 genes were differentially expressed between infected and non-infected macrophages. A total of 71.6% of these genes were down-regulated in the infected macrophages. Functional enrichment analyses identified the steroid and sterol/cholesterol biosynthetic processes between regulatory networks down-regulated in infected macrophages. RT-qPCR further confirmed this down-regulation in genes belonging to these pathways. These findings contrast with those from studies involving other Leishmania species at earlier infection stages, where gene up-regulation for this metabolic pathway has been reported. Sterol biosynthesis could be an important biological process associated with the expression profile of macrophages infected by L. (V.) braziliensis. Differential transcriptional results suggest a negative regulation of the genetic regulatory network involved in cholesterol biosynthesis.
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spelling pubmed-44600722015-06-16 Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection Ovalle-Bracho, Clemencia Franco-Muñoz, Carlos Londoño-Barbosa, Diana Restrepo-Montoya, Daniel Clavijo-Ramírez, Carlos PLoS One Research Article Different Leishmania species cause distinct clinical manifestations of the infectious disease leishmaniasis. It is fundamentally important to understand the mechanisms governing the interaction between Leishmania and its host cell. Little is known about this interaction between Leishmania (Viannia) braziliensis and human macrophages. In this study, we aimed to identify differential gene expression between non-infected and L. (V) braziliensis-infected U937-derived macrophages. We deployed a whole human transcriptome microarray analysis using 72 hours post-infection samples and compared those samples with their non-infected counterparts. We found that 218 genes were differentially expressed between infected and non-infected macrophages. A total of 71.6% of these genes were down-regulated in the infected macrophages. Functional enrichment analyses identified the steroid and sterol/cholesterol biosynthetic processes between regulatory networks down-regulated in infected macrophages. RT-qPCR further confirmed this down-regulation in genes belonging to these pathways. These findings contrast with those from studies involving other Leishmania species at earlier infection stages, where gene up-regulation for this metabolic pathway has been reported. Sterol biosynthesis could be an important biological process associated with the expression profile of macrophages infected by L. (V.) braziliensis. Differential transcriptional results suggest a negative regulation of the genetic regulatory network involved in cholesterol biosynthesis. Public Library of Science 2015-06-08 /pmc/articles/PMC4460072/ /pubmed/26052705 http://dx.doi.org/10.1371/journal.pone.0128934 Text en © 2015 Ovalle-Bracho et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Ovalle-Bracho, Clemencia
Franco-Muñoz, Carlos
Londoño-Barbosa, Diana
Restrepo-Montoya, Daniel
Clavijo-Ramírez, Carlos
Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection
title Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection
title_full Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection
title_fullStr Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection
title_full_unstemmed Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection
title_short Changes in Macrophage Gene Expression Associated with Leishmania (Viannia) braziliensis Infection
title_sort changes in macrophage gene expression associated with leishmania (viannia) braziliensis infection
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460072/
https://www.ncbi.nlm.nih.gov/pubmed/26052705
http://dx.doi.org/10.1371/journal.pone.0128934
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