Nanobodies and recombinant binders in cell biology

Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and hi...

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Detalles Bibliográficos
Autores principales: Helma, Jonas, Cardoso, M. Cristina, Muyldermans, Serge, Leonhardt, Heinrich
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Rockefeller University Press 2015
Materias:
Reviews
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460151/
https://www.ncbi.nlm.nih.gov/pubmed/26056137
http://dx.doi.org/10.1083/jcb.201409074
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author Helma, Jonas
Cardoso, M. Cristina
Muyldermans, Serge
Leonhardt, Heinrich
author_facet Helma, Jonas
Cardoso, M. Cristina
Muyldermans, Serge
Leonhardt, Heinrich
author_sort Helma, Jonas
collection PubMed
description Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes.
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spelling pubmed-44601512015-12-08 Nanobodies and recombinant binders in cell biology Helma, Jonas Cardoso, M. Cristina Muyldermans, Serge Leonhardt, Heinrich J Cell Biol Reviews Antibodies are key reagents to investigate cellular processes. The development of recombinant antibodies and binders derived from natural protein scaffolds has expanded traditional applications, such as immunofluorescence, binding arrays, and immunoprecipitation. In addition, their small size and high stability in ectopic environments have enabled their use in all areas of cell research, including structural biology, advanced microscopy, and intracellular expression. Understanding these novel reagents as genetic modules that can be integrated into cellular pathways opens up a broad experimental spectrum to monitor and manipulate cellular processes. The Rockefeller University Press 2015-06-08 /pmc/articles/PMC4460151/ /pubmed/26056137 http://dx.doi.org/10.1083/jcb.201409074 Text en © 2015 Helma et al. This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 3.0 Unported license, as described at http://creativecommons.org/licenses/by-nc-sa/3.0/).
spellingShingle Reviews
Helma, Jonas
Cardoso, M. Cristina
Muyldermans, Serge
Leonhardt, Heinrich
Nanobodies and recombinant binders in cell biology
title Nanobodies and recombinant binders in cell biology
title_full Nanobodies and recombinant binders in cell biology
title_fullStr Nanobodies and recombinant binders in cell biology
title_full_unstemmed Nanobodies and recombinant binders in cell biology
title_short Nanobodies and recombinant binders in cell biology
title_sort nanobodies and recombinant binders in cell biology
topic Reviews
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460151/
https://www.ncbi.nlm.nih.gov/pubmed/26056137
http://dx.doi.org/10.1083/jcb.201409074
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