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MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells

BACKGROUND: Mounting evidence suggests that miRNAs have major functions in tumor pathogenesis, and this study aimed to identify the candidate miRNA and investigate its role in nasopharyngeal carcinoma (NPC). METHODS: MiRNA and mRNA expressions were screened by microarray assays. The cell proliferati...

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Autores principales: Yang, Wanyong, Lan, Xi, Li, Dongmin, Li, Tao, Lu, Shemin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460644/
https://www.ncbi.nlm.nih.gov/pubmed/26055874
http://dx.doi.org/10.1186/s12885-015-1464-x
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author Yang, Wanyong
Lan, Xi
Li, Dongmin
Li, Tao
Lu, Shemin
author_facet Yang, Wanyong
Lan, Xi
Li, Dongmin
Li, Tao
Lu, Shemin
author_sort Yang, Wanyong
collection PubMed
description BACKGROUND: Mounting evidence suggests that miRNAs have major functions in tumor pathogenesis, and this study aimed to identify the candidate miRNA and investigate its role in nasopharyngeal carcinoma (NPC). METHODS: MiRNA and mRNA expressions were screened by microarray assays. The cell proliferation, colony formation and migration ability were measured by MTT, soft agar and wound healing assays, respectively. The tumor growth suppression was evaluated by xenografting in nude mice. The plasma miR-223 levels in NPC patients were detected by TaqMan analysis. Real-time quantitative PCR and Western blotting were used to confirm miR-223 and MAFB expression levels. The targeting relationship between miR-223 and MAFB was verified using dual luciferase reporter assay. RESULTS: The miR-223 expression was decreased in CNE-1, CNE-2 cells as compared with NP69 cells, an immortalized human nasopharyngeal epithelial cell line, and its level also reduced in NPC patients’ plasma as compared with healthy controls. Exogenous expression of miR-223 in CNE-2 cells could inhibit cell proliferation both in vitro and in vivo. Extrogenous miR-223 in CNE-2 cells would decrease the ability of colony formation and migration. MAFB, a transcription factor of Maf family members, was identified as a target gene of miR-223. We found that migration and invasion abilities were inhibited by MAFB silencing. CONCLUSIONS: MiR-223 negatively regulates the growth and migration of NPC cells via reducing MAFB expression, and this finding provides a novel insight into understanding miR-223 regulation mechanism in nasopharyngeal carcinoma tumorigenesis.
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spelling pubmed-44606442015-06-10 MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells Yang, Wanyong Lan, Xi Li, Dongmin Li, Tao Lu, Shemin BMC Cancer Research Article BACKGROUND: Mounting evidence suggests that miRNAs have major functions in tumor pathogenesis, and this study aimed to identify the candidate miRNA and investigate its role in nasopharyngeal carcinoma (NPC). METHODS: MiRNA and mRNA expressions were screened by microarray assays. The cell proliferation, colony formation and migration ability were measured by MTT, soft agar and wound healing assays, respectively. The tumor growth suppression was evaluated by xenografting in nude mice. The plasma miR-223 levels in NPC patients were detected by TaqMan analysis. Real-time quantitative PCR and Western blotting were used to confirm miR-223 and MAFB expression levels. The targeting relationship between miR-223 and MAFB was verified using dual luciferase reporter assay. RESULTS: The miR-223 expression was decreased in CNE-1, CNE-2 cells as compared with NP69 cells, an immortalized human nasopharyngeal epithelial cell line, and its level also reduced in NPC patients’ plasma as compared with healthy controls. Exogenous expression of miR-223 in CNE-2 cells could inhibit cell proliferation both in vitro and in vivo. Extrogenous miR-223 in CNE-2 cells would decrease the ability of colony formation and migration. MAFB, a transcription factor of Maf family members, was identified as a target gene of miR-223. We found that migration and invasion abilities were inhibited by MAFB silencing. CONCLUSIONS: MiR-223 negatively regulates the growth and migration of NPC cells via reducing MAFB expression, and this finding provides a novel insight into understanding miR-223 regulation mechanism in nasopharyngeal carcinoma tumorigenesis. BioMed Central 2015-06-09 /pmc/articles/PMC4460644/ /pubmed/26055874 http://dx.doi.org/10.1186/s12885-015-1464-x Text en © Yang et al. 2015 This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research Article
Yang, Wanyong
Lan, Xi
Li, Dongmin
Li, Tao
Lu, Shemin
MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells
title MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells
title_full MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells
title_fullStr MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells
title_full_unstemmed MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells
title_short MiR-223 targeting MAFB suppresses proliferation and migration of nasopharyngeal carcinoma cells
title_sort mir-223 targeting mafb suppresses proliferation and migration of nasopharyngeal carcinoma cells
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4460644/
https://www.ncbi.nlm.nih.gov/pubmed/26055874
http://dx.doi.org/10.1186/s12885-015-1464-x
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