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iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants

There is considerable interest in implantation techniques and scaffolds for tissue engineering and, for safety and biocompatibility reasons, inflammation, angiogenesis, and fibrosis need to be determined. The contribution of inducible nitric oxide synthase (iNOS) in the regulation of the foreign bod...

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Autores principales: Cassini-Vieira, Puebla, Araújo, Fernanda Assis, da Costa Dias, Filipi Leles, Russo, Remo Castro, Andrade, Silvia Passos, Teixeira, Mauro Martins, Barcelos, Luciola Silva
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi Publishing Corporation 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461775/
https://www.ncbi.nlm.nih.gov/pubmed/26106257
http://dx.doi.org/10.1155/2015/138461
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author Cassini-Vieira, Puebla
Araújo, Fernanda Assis
da Costa Dias, Filipi Leles
Russo, Remo Castro
Andrade, Silvia Passos
Teixeira, Mauro Martins
Barcelos, Luciola Silva
author_facet Cassini-Vieira, Puebla
Araújo, Fernanda Assis
da Costa Dias, Filipi Leles
Russo, Remo Castro
Andrade, Silvia Passos
Teixeira, Mauro Martins
Barcelos, Luciola Silva
author_sort Cassini-Vieira, Puebla
collection PubMed
description There is considerable interest in implantation techniques and scaffolds for tissue engineering and, for safety and biocompatibility reasons, inflammation, angiogenesis, and fibrosis need to be determined. The contribution of inducible nitric oxide synthase (iNOS) in the regulation of the foreign body reaction induced by subcutaneous implantation of a synthetic matrix was never investigated. Here, we examined the role of iNOS in angiogenesis, inflammation, and collagen deposition induced by polyether-polyurethane synthetic implants, using mice with targeted disruption of the iNOS gene (iNOS(−/−)) and wild-type (WT) mice. The hemoglobin content and number of vessels were decreased in the implants of iNOS(−/−) mice compared to WT mice 14 days after implantation. VEGF levels were also reduced in the implants of iNOS(−/−) mice. In contrast, the iNOS(−/−) implants exhibited an increased neutrophil and macrophage infiltration. However, no alterations were observed in levels of CXCL1 and CCL2, chemokines related to neutrophil and macrophage migration, respectively. Furthermore, the implants of iNOS(−/−) mice showed boosted collagen deposition. These data suggest that iNOS activity controls inflammation, angiogenesis, and fibrogenesis in polyether-polyurethane synthetic implants and that lack of iNOS expression increases foreign body reaction to implants in mice.
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spelling pubmed-44617752015-06-23 iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants Cassini-Vieira, Puebla Araújo, Fernanda Assis da Costa Dias, Filipi Leles Russo, Remo Castro Andrade, Silvia Passos Teixeira, Mauro Martins Barcelos, Luciola Silva Mediators Inflamm Research Article There is considerable interest in implantation techniques and scaffolds for tissue engineering and, for safety and biocompatibility reasons, inflammation, angiogenesis, and fibrosis need to be determined. The contribution of inducible nitric oxide synthase (iNOS) in the regulation of the foreign body reaction induced by subcutaneous implantation of a synthetic matrix was never investigated. Here, we examined the role of iNOS in angiogenesis, inflammation, and collagen deposition induced by polyether-polyurethane synthetic implants, using mice with targeted disruption of the iNOS gene (iNOS(−/−)) and wild-type (WT) mice. The hemoglobin content and number of vessels were decreased in the implants of iNOS(−/−) mice compared to WT mice 14 days after implantation. VEGF levels were also reduced in the implants of iNOS(−/−) mice. In contrast, the iNOS(−/−) implants exhibited an increased neutrophil and macrophage infiltration. However, no alterations were observed in levels of CXCL1 and CCL2, chemokines related to neutrophil and macrophage migration, respectively. Furthermore, the implants of iNOS(−/−) mice showed boosted collagen deposition. These data suggest that iNOS activity controls inflammation, angiogenesis, and fibrogenesis in polyether-polyurethane synthetic implants and that lack of iNOS expression increases foreign body reaction to implants in mice. Hindawi Publishing Corporation 2015 2015-05-27 /pmc/articles/PMC4461775/ /pubmed/26106257 http://dx.doi.org/10.1155/2015/138461 Text en Copyright © 2015 Puebla Cassini-Vieira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Cassini-Vieira, Puebla
Araújo, Fernanda Assis
da Costa Dias, Filipi Leles
Russo, Remo Castro
Andrade, Silvia Passos
Teixeira, Mauro Martins
Barcelos, Luciola Silva
iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
title iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
title_full iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
title_fullStr iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
title_full_unstemmed iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
title_short iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
title_sort inos activity modulates inflammation, angiogenesis, and tissue fibrosis in polyether-polyurethane synthetic implants
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461775/
https://www.ncbi.nlm.nih.gov/pubmed/26106257
http://dx.doi.org/10.1155/2015/138461
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