Cargando…
iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants
There is considerable interest in implantation techniques and scaffolds for tissue engineering and, for safety and biocompatibility reasons, inflammation, angiogenesis, and fibrosis need to be determined. The contribution of inducible nitric oxide synthase (iNOS) in the regulation of the foreign bod...
Autores principales: | , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Hindawi Publishing Corporation
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461775/ https://www.ncbi.nlm.nih.gov/pubmed/26106257 http://dx.doi.org/10.1155/2015/138461 |
_version_ | 1782375552452132864 |
---|---|
author | Cassini-Vieira, Puebla Araújo, Fernanda Assis da Costa Dias, Filipi Leles Russo, Remo Castro Andrade, Silvia Passos Teixeira, Mauro Martins Barcelos, Luciola Silva |
author_facet | Cassini-Vieira, Puebla Araújo, Fernanda Assis da Costa Dias, Filipi Leles Russo, Remo Castro Andrade, Silvia Passos Teixeira, Mauro Martins Barcelos, Luciola Silva |
author_sort | Cassini-Vieira, Puebla |
collection | PubMed |
description | There is considerable interest in implantation techniques and scaffolds for tissue engineering and, for safety and biocompatibility reasons, inflammation, angiogenesis, and fibrosis need to be determined. The contribution of inducible nitric oxide synthase (iNOS) in the regulation of the foreign body reaction induced by subcutaneous implantation of a synthetic matrix was never investigated. Here, we examined the role of iNOS in angiogenesis, inflammation, and collagen deposition induced by polyether-polyurethane synthetic implants, using mice with targeted disruption of the iNOS gene (iNOS(−/−)) and wild-type (WT) mice. The hemoglobin content and number of vessels were decreased in the implants of iNOS(−/−) mice compared to WT mice 14 days after implantation. VEGF levels were also reduced in the implants of iNOS(−/−) mice. In contrast, the iNOS(−/−) implants exhibited an increased neutrophil and macrophage infiltration. However, no alterations were observed in levels of CXCL1 and CCL2, chemokines related to neutrophil and macrophage migration, respectively. Furthermore, the implants of iNOS(−/−) mice showed boosted collagen deposition. These data suggest that iNOS activity controls inflammation, angiogenesis, and fibrogenesis in polyether-polyurethane synthetic implants and that lack of iNOS expression increases foreign body reaction to implants in mice. |
format | Online Article Text |
id | pubmed-4461775 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Hindawi Publishing Corporation |
record_format | MEDLINE/PubMed |
spelling | pubmed-44617752015-06-23 iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants Cassini-Vieira, Puebla Araújo, Fernanda Assis da Costa Dias, Filipi Leles Russo, Remo Castro Andrade, Silvia Passos Teixeira, Mauro Martins Barcelos, Luciola Silva Mediators Inflamm Research Article There is considerable interest in implantation techniques and scaffolds for tissue engineering and, for safety and biocompatibility reasons, inflammation, angiogenesis, and fibrosis need to be determined. The contribution of inducible nitric oxide synthase (iNOS) in the regulation of the foreign body reaction induced by subcutaneous implantation of a synthetic matrix was never investigated. Here, we examined the role of iNOS in angiogenesis, inflammation, and collagen deposition induced by polyether-polyurethane synthetic implants, using mice with targeted disruption of the iNOS gene (iNOS(−/−)) and wild-type (WT) mice. The hemoglobin content and number of vessels were decreased in the implants of iNOS(−/−) mice compared to WT mice 14 days after implantation. VEGF levels were also reduced in the implants of iNOS(−/−) mice. In contrast, the iNOS(−/−) implants exhibited an increased neutrophil and macrophage infiltration. However, no alterations were observed in levels of CXCL1 and CCL2, chemokines related to neutrophil and macrophage migration, respectively. Furthermore, the implants of iNOS(−/−) mice showed boosted collagen deposition. These data suggest that iNOS activity controls inflammation, angiogenesis, and fibrogenesis in polyether-polyurethane synthetic implants and that lack of iNOS expression increases foreign body reaction to implants in mice. Hindawi Publishing Corporation 2015 2015-05-27 /pmc/articles/PMC4461775/ /pubmed/26106257 http://dx.doi.org/10.1155/2015/138461 Text en Copyright © 2015 Puebla Cassini-Vieira et al. https://creativecommons.org/licenses/by/3.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Article Cassini-Vieira, Puebla Araújo, Fernanda Assis da Costa Dias, Filipi Leles Russo, Remo Castro Andrade, Silvia Passos Teixeira, Mauro Martins Barcelos, Luciola Silva iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants |
title | iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants |
title_full | iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants |
title_fullStr | iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants |
title_full_unstemmed | iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants |
title_short | iNOS Activity Modulates Inflammation, Angiogenesis, and Tissue Fibrosis in Polyether-Polyurethane Synthetic Implants |
title_sort | inos activity modulates inflammation, angiogenesis, and tissue fibrosis in polyether-polyurethane synthetic implants |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461775/ https://www.ncbi.nlm.nih.gov/pubmed/26106257 http://dx.doi.org/10.1155/2015/138461 |
work_keys_str_mv | AT cassinivieirapuebla inosactivitymodulatesinflammationangiogenesisandtissuefibrosisinpolyetherpolyurethanesyntheticimplants AT araujofernandaassis inosactivitymodulatesinflammationangiogenesisandtissuefibrosisinpolyetherpolyurethanesyntheticimplants AT dacostadiasfilipileles inosactivitymodulatesinflammationangiogenesisandtissuefibrosisinpolyetherpolyurethanesyntheticimplants AT russoremocastro inosactivitymodulatesinflammationangiogenesisandtissuefibrosisinpolyetherpolyurethanesyntheticimplants AT andradesilviapassos inosactivitymodulatesinflammationangiogenesisandtissuefibrosisinpolyetherpolyurethanesyntheticimplants AT teixeiramauromartins inosactivitymodulatesinflammationangiogenesisandtissuefibrosisinpolyetherpolyurethanesyntheticimplants AT barcelosluciolasilva inosactivitymodulatesinflammationangiogenesisandtissuefibrosisinpolyetherpolyurethanesyntheticimplants |