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Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
BACKGROUND: Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
BioMed Central
2015
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461904/ https://www.ncbi.nlm.nih.gov/pubmed/25980573 http://dx.doi.org/10.1186/s12951-015-0096-2 |
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author | Pan, Jian-feng Liu, Ning-hua Shu, Lin-yuan Sun, Hui |
author_facet | Pan, Jian-feng Liu, Ning-hua Shu, Lin-yuan Sun, Hui |
author_sort | Pan, Jian-feng |
collection | PubMed |
description | BACKGROUND: Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time. RESULTS: PLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously. CONCLUSIONS: PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area. |
format | Online Article Text |
id | pubmed-4461904 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | BioMed Central |
record_format | MEDLINE/PubMed |
spelling | pubmed-44619042015-06-11 Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices Pan, Jian-feng Liu, Ning-hua Shu, Lin-yuan Sun, Hui J Nanobiotechnology Research BACKGROUND: Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time. RESULTS: PLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously. CONCLUSIONS: PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area. BioMed Central 2015-05-16 /pmc/articles/PMC4461904/ /pubmed/25980573 http://dx.doi.org/10.1186/s12951-015-0096-2 Text en © Pan et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated. |
spellingShingle | Research Pan, Jian-feng Liu, Ning-hua Shu, Lin-yuan Sun, Hui Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices |
title | Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices |
title_full | Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices |
title_fullStr | Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices |
title_full_unstemmed | Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices |
title_short | Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices |
title_sort | application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461904/ https://www.ncbi.nlm.nih.gov/pubmed/25980573 http://dx.doi.org/10.1186/s12951-015-0096-2 |
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