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Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices

BACKGROUND: Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is...

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Autores principales: Pan, Jian-feng, Liu, Ning-hua, Shu, Lin-yuan, Sun, Hui
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461904/
https://www.ncbi.nlm.nih.gov/pubmed/25980573
http://dx.doi.org/10.1186/s12951-015-0096-2
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author Pan, Jian-feng
Liu, Ning-hua
Shu, Lin-yuan
Sun, Hui
author_facet Pan, Jian-feng
Liu, Ning-hua
Shu, Lin-yuan
Sun, Hui
author_sort Pan, Jian-feng
collection PubMed
description BACKGROUND: Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time. RESULTS: PLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously. CONCLUSIONS: PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area.
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spelling pubmed-44619042015-06-11 Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices Pan, Jian-feng Liu, Ning-hua Shu, Lin-yuan Sun, Hui J Nanobiotechnology Research BACKGROUND: Electrospinning is an easy and effective technique to produce submicron fibers possessing a range of attractive characteristics such as interconnected porous structures similar to natural ECM and good resilience to movement. Rapid and efficient cell attachment to nanofibrous matrices is a necessary prerequisite in tissue engineering. Thus, the aim of this study is to evaluate poly(ε-caprolactone-co-lactide)/Pluronic (PLCL/Pluronic) nanofibrous matrices with avidin-biotin technology for improving cell adhesion for the first time. RESULTS: PLCL/Pluronic nanofibers had relatively homogeneous fibers and interconnected porous structures. Pluronic significantly modified the hydrophilicity of nanofibrous matrices and PLCL/Pluronic nanofibrous matrices had better performance on maintaining cell proliferation. Avidin-biotin technology had no negative effect on the hydrophilic property, mechanical property and cell proliferation. Meanwhile, the attachment and spreading of adipose-derived stem cells (ADSCs) onto PLCL/Pluronic nanofibrous matrices with avidin-biotin technology was promoted obviously. CONCLUSIONS: PLCL/Pluronic nanofibrous matrices inheriting the excellent characteristics of both PLCL and Pluronic have the better cell adhesion ability through avidin-biotin technology, implying a promising application in skin care, tissue regeneration and other related area. BioMed Central 2015-05-16 /pmc/articles/PMC4461904/ /pubmed/25980573 http://dx.doi.org/10.1186/s12951-015-0096-2 Text en © Pan et al.; licensee BioMed Central. 2015 This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
spellingShingle Research
Pan, Jian-feng
Liu, Ning-hua
Shu, Lin-yuan
Sun, Hui
Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
title Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
title_full Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
title_fullStr Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
title_full_unstemmed Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
title_short Application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
title_sort application of avidin-biotin technology to improve cell adhesion on nanofibrous matrices
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461904/
https://www.ncbi.nlm.nih.gov/pubmed/25980573
http://dx.doi.org/10.1186/s12951-015-0096-2
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