Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division

Asymmetric distribution of fate determinants within cells is an essential biological strategy to prepare them for asymmetric division. In this work we measure the intracellular distribution of 12 maternal microRNAs (miRNA) along the animal-vegetal axis of the Xenopus laevis oocyte using qPCR tomogra...

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Autores principales: Sidova, Monika, Sindelka, Radek, Castoldi, Mirco, Benes, Vladimir, Kubista, Mikael
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461913/
https://www.ncbi.nlm.nih.gov/pubmed/26059897
http://dx.doi.org/10.1038/srep11157
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author Sidova, Monika
Sindelka, Radek
Castoldi, Mirco
Benes, Vladimir
Kubista, Mikael
author_facet Sidova, Monika
Sindelka, Radek
Castoldi, Mirco
Benes, Vladimir
Kubista, Mikael
author_sort Sidova, Monika
collection PubMed
description Asymmetric distribution of fate determinants within cells is an essential biological strategy to prepare them for asymmetric division. In this work we measure the intracellular distribution of 12 maternal microRNAs (miRNA) along the animal-vegetal axis of the Xenopus laevis oocyte using qPCR tomography. We find the miRNAs have distinct intracellular profiles that resemble two out of the three profiles we previously observed for mRNAs. Our results suggest that miRNAs in addition to proteins and mRNAs may have asymmetric distribution within the oocyte and may contribute to asymmetric cell division as cell fate determinants.
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spelling pubmed-44619132015-06-12 Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division Sidova, Monika Sindelka, Radek Castoldi, Mirco Benes, Vladimir Kubista, Mikael Sci Rep Article Asymmetric distribution of fate determinants within cells is an essential biological strategy to prepare them for asymmetric division. In this work we measure the intracellular distribution of 12 maternal microRNAs (miRNA) along the animal-vegetal axis of the Xenopus laevis oocyte using qPCR tomography. We find the miRNAs have distinct intracellular profiles that resemble two out of the three profiles we previously observed for mRNAs. Our results suggest that miRNAs in addition to proteins and mRNAs may have asymmetric distribution within the oocyte and may contribute to asymmetric cell division as cell fate determinants. Nature Publishing Group 2015-06-10 /pmc/articles/PMC4461913/ /pubmed/26059897 http://dx.doi.org/10.1038/srep11157 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Sidova, Monika
Sindelka, Radek
Castoldi, Mirco
Benes, Vladimir
Kubista, Mikael
Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division
title Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division
title_full Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division
title_fullStr Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division
title_full_unstemmed Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division
title_short Intracellular microRNA profiles form in the Xenopus laevis oocyte that may contribute to asymmetric cell division
title_sort intracellular microrna profiles form in the xenopus laevis oocyte that may contribute to asymmetric cell division
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461913/
https://www.ncbi.nlm.nih.gov/pubmed/26059897
http://dx.doi.org/10.1038/srep11157
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