Cargando…
Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties
BACKGROUND: Mushrooms have been valued for their nutritive content and as traditional medicines; several important medicinal properties of mushrooms have been recognized worldwide. OBJECTIVE: The purpose of this study was to elucidate the cell growth inhibitory potential of four edible mushrooms; Co...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Medknow Publications & Media Pvt Ltd
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461969/ https://www.ncbi.nlm.nih.gov/pubmed/26109775 http://dx.doi.org/10.4103/0973-1296.157665 |
_version_ | 1782375584205111296 |
---|---|
author | Sadi, Gökhan Emsen, Buğrahan Kaya, Abdullah Kocabaş, Aytaç Çınar, Seval Kartal, Deniz İrtem |
author_facet | Sadi, Gökhan Emsen, Buğrahan Kaya, Abdullah Kocabaş, Aytaç Çınar, Seval Kartal, Deniz İrtem |
author_sort | Sadi, Gökhan |
collection | PubMed |
description | BACKGROUND: Mushrooms have been valued for their nutritive content and as traditional medicines; several important medicinal properties of mushrooms have been recognized worldwide. OBJECTIVE: The purpose of this study was to elucidate the cell growth inhibitory potential of four edible mushrooms; Coprinus comatus (O.F. Mull.) Pers. (Agaricaceae), Tricholoma fracticum (Britzelm.) Kreisel (Tricholomataceae), Rhizopogon luteolus Fr. and Nordholm (Rhizopogonaceae), Lentinus tigrinus (Bull.) Fr. (Polyporaceae) on hepatocellular carcinoma (HepG2) cells in conjunction with their antioxidant and antibacterial capacities. MATERIALS AND METHODS: Five different extracts of edible mushrooms were obtained using water, methanol, acetone, n-hexane and chloroform as solvent systems for cytotoxic, antioxidant and antibacterial properties. RESULTS: C. comatus showed substantial in vitro cytotoxic activity against HepG2 cell lines with all extracts especially with chloroform 50% inhibition (IC(50) value of 0.086 mg/ml) and acetone (IC(50) value of 0.420 mg/ml). Chloroform extract of C. comatus had maximum amount of β-carotene (25.94 μg/mg), total phenolic content (76.32 μg/mg) and lycopene (12.00 μg/mg), and n-hexane extract of L. tigrinus had maximum amount of flavonoid (3.67 μg/mg). While chloroform extract of C. comatus showed the highest 2, 2-diphenyl-1-picrylhydrazyl (DPPH) capturing activity (1.579 mg/ml), the best result for metal chelating activity was obtained from methanolic extract (0.842 mg/ml). Moreover, all tested mushrooms demonstrated antibacterial activity and n-hexane extract of L. tigrinus and acetone extracts of T. fracticum were the most active against tested microorganism. CONCLUSION: These results indicate that different extracts of investigated mushroom have considerable cytotoxic, antioxidant and antibacterial properties and may be utilized as a promising source of therapeutics. |
format | Online Article Text |
id | pubmed-4461969 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Medknow Publications & Media Pvt Ltd |
record_format | MEDLINE/PubMed |
spelling | pubmed-44619692015-06-24 Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties Sadi, Gökhan Emsen, Buğrahan Kaya, Abdullah Kocabaş, Aytaç Çınar, Seval Kartal, Deniz İrtem Pharmacogn Mag Original Article BACKGROUND: Mushrooms have been valued for their nutritive content and as traditional medicines; several important medicinal properties of mushrooms have been recognized worldwide. OBJECTIVE: The purpose of this study was to elucidate the cell growth inhibitory potential of four edible mushrooms; Coprinus comatus (O.F. Mull.) Pers. (Agaricaceae), Tricholoma fracticum (Britzelm.) Kreisel (Tricholomataceae), Rhizopogon luteolus Fr. and Nordholm (Rhizopogonaceae), Lentinus tigrinus (Bull.) Fr. (Polyporaceae) on hepatocellular carcinoma (HepG2) cells in conjunction with their antioxidant and antibacterial capacities. MATERIALS AND METHODS: Five different extracts of edible mushrooms were obtained using water, methanol, acetone, n-hexane and chloroform as solvent systems for cytotoxic, antioxidant and antibacterial properties. RESULTS: C. comatus showed substantial in vitro cytotoxic activity against HepG2 cell lines with all extracts especially with chloroform 50% inhibition (IC(50) value of 0.086 mg/ml) and acetone (IC(50) value of 0.420 mg/ml). Chloroform extract of C. comatus had maximum amount of β-carotene (25.94 μg/mg), total phenolic content (76.32 μg/mg) and lycopene (12.00 μg/mg), and n-hexane extract of L. tigrinus had maximum amount of flavonoid (3.67 μg/mg). While chloroform extract of C. comatus showed the highest 2, 2-diphenyl-1-picrylhydrazyl (DPPH) capturing activity (1.579 mg/ml), the best result for metal chelating activity was obtained from methanolic extract (0.842 mg/ml). Moreover, all tested mushrooms demonstrated antibacterial activity and n-hexane extract of L. tigrinus and acetone extracts of T. fracticum were the most active against tested microorganism. CONCLUSION: These results indicate that different extracts of investigated mushroom have considerable cytotoxic, antioxidant and antibacterial properties and may be utilized as a promising source of therapeutics. Medknow Publications & Media Pvt Ltd 2015-05 /pmc/articles/PMC4461969/ /pubmed/26109775 http://dx.doi.org/10.4103/0973-1296.157665 Text en Copyright: © Pharmacognosy Magazine http://creativecommons.org/licenses/by-nc-sa/3.0 This is an open-access article distributed under the terms of the Creative Commons Attribution-Noncommercial-Share Alike 3.0 Unported, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Original Article Sadi, Gökhan Emsen, Buğrahan Kaya, Abdullah Kocabaş, Aytaç Çınar, Seval Kartal, Deniz İrtem Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties |
title | Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties |
title_full | Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties |
title_fullStr | Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties |
title_full_unstemmed | Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties |
title_short | Cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties |
title_sort | cytotoxicity of some edible mushrooms extracts over liver hepatocellular carcinoma cells in conjunction with their antioxidant and antibacterial properties |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4461969/ https://www.ncbi.nlm.nih.gov/pubmed/26109775 http://dx.doi.org/10.4103/0973-1296.157665 |
work_keys_str_mv | AT sadigokhan cytotoxicityofsomeediblemushroomsextractsoverliverhepatocellularcarcinomacellsinconjunctionwiththeirantioxidantandantibacterialproperties AT emsenbugrahan cytotoxicityofsomeediblemushroomsextractsoverliverhepatocellularcarcinomacellsinconjunctionwiththeirantioxidantandantibacterialproperties AT kayaabdullah cytotoxicityofsomeediblemushroomsextractsoverliverhepatocellularcarcinomacellsinconjunctionwiththeirantioxidantandantibacterialproperties AT kocabasaytac cytotoxicityofsomeediblemushroomsextractsoverliverhepatocellularcarcinomacellsinconjunctionwiththeirantioxidantandantibacterialproperties AT cınarseval cytotoxicityofsomeediblemushroomsextractsoverliverhepatocellularcarcinomacellsinconjunctionwiththeirantioxidantandantibacterialproperties AT kartaldenizirtem cytotoxicityofsomeediblemushroomsextractsoverliverhepatocellularcarcinomacellsinconjunctionwiththeirantioxidantandantibacterialproperties |