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DNA transducer-triggered signal switch for visual colorimetric bioanalysis

A simple and versatile colorimetric biosensor has been developed for sensitive and specific detection of a wide range of biomolecules, such as oligonucleotides and aptamer-recognized targets. Combining the signal transducer and catalyzed hairpin assembly (CHA)-based signal amplification, the target...

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Autores principales: Chen, Wenhong, Yan, Yurong, Zhang, Ye, Zhang, Xuemei, Yin, Yibing, Ding, Shijia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462091/
https://www.ncbi.nlm.nih.gov/pubmed/26060886
http://dx.doi.org/10.1038/srep11190
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author Chen, Wenhong
Yan, Yurong
Zhang, Ye
Zhang, Xuemei
Yin, Yibing
Ding, Shijia
author_facet Chen, Wenhong
Yan, Yurong
Zhang, Ye
Zhang, Xuemei
Yin, Yibing
Ding, Shijia
author_sort Chen, Wenhong
collection PubMed
description A simple and versatile colorimetric biosensor has been developed for sensitive and specific detection of a wide range of biomolecules, such as oligonucleotides and aptamer-recognized targets. Combining the signal transducer and catalyzed hairpin assembly (CHA)-based signal amplification, the target DNA binds with the hairpin DNA to form a new nucleic acid sequence and creates a toehold in the transducer for initiating the recycle amplification reaction of CHA. The catalyzed assembly process produces a large amount of G-rich DNA. In the presence of hemin, the G-rich DNA forms G-quadruplex/hemin complex and mimic horseradish peroxidase activity, which catalyzes a colorimetric reaction. Under optimal conditions, the calibration curve of synthetic target DNA has good linearity from 50 pM to 200 nM with a detection limit of 32 pM. This strategy has been successfully applied to detect S. pneumoniae as low as 156 CFU mL(−1), and shows a good specificity against closely related streptococci and major pathogenic bacteria. In addition, the developed method enables successful visual analysis of S. pneumoniae in clinical samples by the naked eye. Importantly, this method demonstrates excellent assay versatility for sensitively detecting oligonucleotides or aptamer-recognized targets.
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spelling pubmed-44620912015-06-12 DNA transducer-triggered signal switch for visual colorimetric bioanalysis Chen, Wenhong Yan, Yurong Zhang, Ye Zhang, Xuemei Yin, Yibing Ding, Shijia Sci Rep Article A simple and versatile colorimetric biosensor has been developed for sensitive and specific detection of a wide range of biomolecules, such as oligonucleotides and aptamer-recognized targets. Combining the signal transducer and catalyzed hairpin assembly (CHA)-based signal amplification, the target DNA binds with the hairpin DNA to form a new nucleic acid sequence and creates a toehold in the transducer for initiating the recycle amplification reaction of CHA. The catalyzed assembly process produces a large amount of G-rich DNA. In the presence of hemin, the G-rich DNA forms G-quadruplex/hemin complex and mimic horseradish peroxidase activity, which catalyzes a colorimetric reaction. Under optimal conditions, the calibration curve of synthetic target DNA has good linearity from 50 pM to 200 nM with a detection limit of 32 pM. This strategy has been successfully applied to detect S. pneumoniae as low as 156 CFU mL(−1), and shows a good specificity against closely related streptococci and major pathogenic bacteria. In addition, the developed method enables successful visual analysis of S. pneumoniae in clinical samples by the naked eye. Importantly, this method demonstrates excellent assay versatility for sensitively detecting oligonucleotides or aptamer-recognized targets. Nature Publishing Group 2015-06-10 /pmc/articles/PMC4462091/ /pubmed/26060886 http://dx.doi.org/10.1038/srep11190 Text en Copyright © 2015, Macmillan Publishers Limited http://creativecommons.org/licenses/by/4.0/ This work is licensed under a Creative Commons Attribution 4.0 International License. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in the credit line; if the material is not included under the Creative Commons license, users will need to obtain permission from the license holder to reproduce the material. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/
spellingShingle Article
Chen, Wenhong
Yan, Yurong
Zhang, Ye
Zhang, Xuemei
Yin, Yibing
Ding, Shijia
DNA transducer-triggered signal switch for visual colorimetric bioanalysis
title DNA transducer-triggered signal switch for visual colorimetric bioanalysis
title_full DNA transducer-triggered signal switch for visual colorimetric bioanalysis
title_fullStr DNA transducer-triggered signal switch for visual colorimetric bioanalysis
title_full_unstemmed DNA transducer-triggered signal switch for visual colorimetric bioanalysis
title_short DNA transducer-triggered signal switch for visual colorimetric bioanalysis
title_sort dna transducer-triggered signal switch for visual colorimetric bioanalysis
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462091/
https://www.ncbi.nlm.nih.gov/pubmed/26060886
http://dx.doi.org/10.1038/srep11190
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