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Induction of a chronic myocardial infarction in the laboratory animal - experimental model

INTRODUCTION: Ischemic heart disease is a major public health problem in western countries. Appropriate animal experimental models of chronic myocardial infarction is an essential first step in order to investigate and develop new therapeutic interventions. AIM: The aim of this study was to find an...

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Autores principales: POP, IONEL CIPRIAN, GRAD, NICOLAE-OVIDIU, PESTEAN, COSMIN, TAULESCU, MARIAN, MIRCEAN, MIRCEA, MIRONIUC, ION-AUREL
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Iuliu Hatieganu University of Medicine and Pharmacy 2013
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462451/
https://www.ncbi.nlm.nih.gov/pubmed/26527973
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author POP, IONEL CIPRIAN
GRAD, NICOLAE-OVIDIU
PESTEAN, COSMIN
TAULESCU, MARIAN
MIRCEAN, MIRCEA
MIRONIUC, ION-AUREL
author_facet POP, IONEL CIPRIAN
GRAD, NICOLAE-OVIDIU
PESTEAN, COSMIN
TAULESCU, MARIAN
MIRCEAN, MIRCEA
MIRONIUC, ION-AUREL
author_sort POP, IONEL CIPRIAN
collection PubMed
description INTRODUCTION: Ischemic heart disease is a major public health problem in western countries. Appropriate animal experimental models of chronic myocardial infarction is an essential first step in order to investigate and develop new therapeutic interventions. AIM: The aim of this study was to find an optimal place for a coronary artery ligation to induce an optimal chronic myocardial infarction and also a new heart approach that will not require oro-tracheal intubation. MATERIAL AND METHODS: To achieve these goals we used a group of rabbits and after induction of anesthesia and cardiac exposure by rib osteotomy (rib III, IV and V) at the costo-sternal junction level on the right side we performed three different left anterior descending artery (LAD) ligation at different distances (5, 10 and 15 mm) in relation to the apex. Thirty days after the acute myocardial infarction, we correlated laboratory investigations (serology, ECG, cardiac ultrasound) with histopathological findings. RESULTS: Heart approach achieved by rib osteotomy (rib III, IV and V) at the costo-sternal junction level on the right side, maintains the integrity of the ribcage, allowing it to take part in respiratory movements and the animal model does not need oro-tracheal intubation. Ligation of LAD at 15 mm from the apex was incompatible with life; ligation of LAD at 5 mm from the apex does not achieved transmural myocardial infarction and ligation of LAD at 10 mm from the apex achieved a transmural myocardial infarction of the left ventricle which also involved the distal part of the interventricular septum. CONCLUSION: Ligation of LAD at 10 mm from the apex achieved a transmural myocardial infarction of the left ventricle, is in an easily accessible area from technical point of view, it is sufficiently expanded to induce hemodynamic effects that can be quantified with paraclinical examination and also it is compatible with the experimental animal life. If the heart is approached by rib III, IV and V osteotomy at the costo-sternal junction level on the right side combined with neuroleptic anaesthesia, the animal does not need assisted ventilation.
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spelling pubmed-44624512015-11-02 Induction of a chronic myocardial infarction in the laboratory animal - experimental model POP, IONEL CIPRIAN GRAD, NICOLAE-OVIDIU PESTEAN, COSMIN TAULESCU, MARIAN MIRCEAN, MIRCEA MIRONIUC, ION-AUREL Clujul Med Original Research INTRODUCTION: Ischemic heart disease is a major public health problem in western countries. Appropriate animal experimental models of chronic myocardial infarction is an essential first step in order to investigate and develop new therapeutic interventions. AIM: The aim of this study was to find an optimal place for a coronary artery ligation to induce an optimal chronic myocardial infarction and also a new heart approach that will not require oro-tracheal intubation. MATERIAL AND METHODS: To achieve these goals we used a group of rabbits and after induction of anesthesia and cardiac exposure by rib osteotomy (rib III, IV and V) at the costo-sternal junction level on the right side we performed three different left anterior descending artery (LAD) ligation at different distances (5, 10 and 15 mm) in relation to the apex. Thirty days after the acute myocardial infarction, we correlated laboratory investigations (serology, ECG, cardiac ultrasound) with histopathological findings. RESULTS: Heart approach achieved by rib osteotomy (rib III, IV and V) at the costo-sternal junction level on the right side, maintains the integrity of the ribcage, allowing it to take part in respiratory movements and the animal model does not need oro-tracheal intubation. Ligation of LAD at 15 mm from the apex was incompatible with life; ligation of LAD at 5 mm from the apex does not achieved transmural myocardial infarction and ligation of LAD at 10 mm from the apex achieved a transmural myocardial infarction of the left ventricle which also involved the distal part of the interventricular septum. CONCLUSION: Ligation of LAD at 10 mm from the apex achieved a transmural myocardial infarction of the left ventricle, is in an easily accessible area from technical point of view, it is sufficiently expanded to induce hemodynamic effects that can be quantified with paraclinical examination and also it is compatible with the experimental animal life. If the heart is approached by rib III, IV and V osteotomy at the costo-sternal junction level on the right side combined with neuroleptic anaesthesia, the animal does not need assisted ventilation. Iuliu Hatieganu University of Medicine and Pharmacy 2013 2013-11-06 /pmc/articles/PMC4462451/ /pubmed/26527973 Text en http://creativecommons.org/licenses/by-nc-nd/4.0/ This work is licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 International License
spellingShingle Original Research
POP, IONEL CIPRIAN
GRAD, NICOLAE-OVIDIU
PESTEAN, COSMIN
TAULESCU, MARIAN
MIRCEAN, MIRCEA
MIRONIUC, ION-AUREL
Induction of a chronic myocardial infarction in the laboratory animal - experimental model
title Induction of a chronic myocardial infarction in the laboratory animal - experimental model
title_full Induction of a chronic myocardial infarction in the laboratory animal - experimental model
title_fullStr Induction of a chronic myocardial infarction in the laboratory animal - experimental model
title_full_unstemmed Induction of a chronic myocardial infarction in the laboratory animal - experimental model
title_short Induction of a chronic myocardial infarction in the laboratory animal - experimental model
title_sort induction of a chronic myocardial infarction in the laboratory animal - experimental model
topic Original Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462451/
https://www.ncbi.nlm.nih.gov/pubmed/26527973
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