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Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin

We observed that oleuropein, the main constituent of the leaves and unprocessed olive drupes of Olea europaea, protected mice from high-fat diet-induced adiposity by up-regulation of genes involved in Wnt10b-mediated signaling in adipose tissue. The activation of Wnt/β-catenin pathway is also well e...

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Autores principales: Tong, Tao, Kim, Nahyun, Park, Taesun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462586/
https://www.ncbi.nlm.nih.gov/pubmed/26060936
http://dx.doi.org/10.1371/journal.pone.0129578
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author Tong, Tao
Kim, Nahyun
Park, Taesun
author_facet Tong, Tao
Kim, Nahyun
Park, Taesun
author_sort Tong, Tao
collection PubMed
description We observed that oleuropein, the main constituent of the leaves and unprocessed olive drupes of Olea europaea, protected mice from high-fat diet-induced adiposity by up-regulation of genes involved in Wnt10b-mediated signaling in adipose tissue. The activation of Wnt/β-catenin pathway is also well established to positively regulate the anagen phase of hair growth cycle in mice skin. METHODOLOGY AND PRINCIPAL FINDINGS: Oleuropein promoted cultured human follicle dermal papilla cell proliferation and induced LEF1 and Cyc-D1 mRNA expression and β-catenin protein expression in dermal papilla cells. Nuclear accumulation of β-catenin in dermal papilla cells was observed after oleuropein treatment. Topical application of oleuropein (0.4 mg/mouse/day) to C57BL/6N mice accelerated the hair-growth induction and increased the size of hair follicles in telogenic mouse skin. The oleuropein-treated mouse skin showed substantial upregulation of Wnt10b, FZDR1, LRP5, LEF1, Cyc-D1, IGF-1, KGF, HGF, and VEGF mRNA expression and β-catenin protein expression. CONCLUSIONS AND SIGNIFICANCE: These results demonstrate that topical oleuroepin administration induced anagenic hair growth in telogenic C57BL/6N mouse skin. The hair-growth promoting effect of oleuropein in mice appeared to be associated with the stimulation of the Wnt10b/β-catenin signaling pathway and the upregulation of IGF-1, KGF, HGF, and VEGF gene expression in mouse skin tissue.
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spelling pubmed-44625862015-06-25 Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin Tong, Tao Kim, Nahyun Park, Taesun PLoS One Research Article We observed that oleuropein, the main constituent of the leaves and unprocessed olive drupes of Olea europaea, protected mice from high-fat diet-induced adiposity by up-regulation of genes involved in Wnt10b-mediated signaling in adipose tissue. The activation of Wnt/β-catenin pathway is also well established to positively regulate the anagen phase of hair growth cycle in mice skin. METHODOLOGY AND PRINCIPAL FINDINGS: Oleuropein promoted cultured human follicle dermal papilla cell proliferation and induced LEF1 and Cyc-D1 mRNA expression and β-catenin protein expression in dermal papilla cells. Nuclear accumulation of β-catenin in dermal papilla cells was observed after oleuropein treatment. Topical application of oleuropein (0.4 mg/mouse/day) to C57BL/6N mice accelerated the hair-growth induction and increased the size of hair follicles in telogenic mouse skin. The oleuropein-treated mouse skin showed substantial upregulation of Wnt10b, FZDR1, LRP5, LEF1, Cyc-D1, IGF-1, KGF, HGF, and VEGF mRNA expression and β-catenin protein expression. CONCLUSIONS AND SIGNIFICANCE: These results demonstrate that topical oleuroepin administration induced anagenic hair growth in telogenic C57BL/6N mouse skin. The hair-growth promoting effect of oleuropein in mice appeared to be associated with the stimulation of the Wnt10b/β-catenin signaling pathway and the upregulation of IGF-1, KGF, HGF, and VEGF gene expression in mouse skin tissue. Public Library of Science 2015-06-10 /pmc/articles/PMC4462586/ /pubmed/26060936 http://dx.doi.org/10.1371/journal.pone.0129578 Text en © 2015 Tong et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.
spellingShingle Research Article
Tong, Tao
Kim, Nahyun
Park, Taesun
Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin
title Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin
title_full Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin
title_fullStr Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin
title_full_unstemmed Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin
title_short Topical Application of Oleuropein Induces Anagen Hair Growth in Telogen Mouse Skin
title_sort topical application of oleuropein induces anagen hair growth in telogen mouse skin
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462586/
https://www.ncbi.nlm.nih.gov/pubmed/26060936
http://dx.doi.org/10.1371/journal.pone.0129578
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