Cargando…
Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei
Melioidosis results from an infection with the soil-borne pathogen Burkholderia pseudomallei, and cases of melioidosis usually cluster after rains or a typhoon. In an endemic area of Taiwan, B. pseudomallei is primarily geographically distributed in cropped fields in the northwest of this area, wher...
Autores principales: | , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2015
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462588/ https://www.ncbi.nlm.nih.gov/pubmed/26061639 http://dx.doi.org/10.1371/journal.pntd.0003834 |
_version_ | 1782375686104678400 |
---|---|
author | Chen, Pei-Shih Chen, Yao-Shen Lin, Hsi-Hsun Liu, Pei-Ju Ni, Wei-Fan Hsueh, Pei-Tan Liang, Shih-Hsiung Chen, Chialin Chen, Ya-Lei |
author_facet | Chen, Pei-Shih Chen, Yao-Shen Lin, Hsi-Hsun Liu, Pei-Ju Ni, Wei-Fan Hsueh, Pei-Tan Liang, Shih-Hsiung Chen, Chialin Chen, Ya-Lei |
author_sort | Chen, Pei-Shih |
collection | PubMed |
description | Melioidosis results from an infection with the soil-borne pathogen Burkholderia pseudomallei, and cases of melioidosis usually cluster after rains or a typhoon. In an endemic area of Taiwan, B. pseudomallei is primarily geographically distributed in cropped fields in the northwest of this area, whereas melioidosis cases are distributed in a densely populated district in the southeast. We hypothesized that contaminated cropped fields generated aerosols contaminated with B. pseudomallei, which were carried by a northwesterly wind to the densely populated southeastern district. We collected soil and aerosol samples from a 72 km(2) area of land, including the melioidosis-clustered area and its surroundings. Aerosols that contained B. pseudomallei-specific TTSS (type III secretion system) ORF2 DNA were well distributed in the endemic area but were rare in the surrounding areas during the rainy season. The concentration of this specific DNA in aerosols was positively correlated with the incidence of melioidosis and the appearance of a northwesterly wind. Moreover, the isolation rate in the superficial layers of the contaminated cropped field in the northwest was correlated with PCR positivity for aerosols collected from the southeast over a 2-year period. According to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analyses, PFGE Type Ia (ST58) was the predominant pattern linking the molecular association among soil, aerosol and human isolates. Thus, the airborne transmission of melioidosis moves from the contaminated soil to aerosols and/or to humans in this endemic area. |
format | Online Article Text |
id | pubmed-4462588 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2015 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-44625882015-06-25 Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei Chen, Pei-Shih Chen, Yao-Shen Lin, Hsi-Hsun Liu, Pei-Ju Ni, Wei-Fan Hsueh, Pei-Tan Liang, Shih-Hsiung Chen, Chialin Chen, Ya-Lei PLoS Negl Trop Dis Research Article Melioidosis results from an infection with the soil-borne pathogen Burkholderia pseudomallei, and cases of melioidosis usually cluster after rains or a typhoon. In an endemic area of Taiwan, B. pseudomallei is primarily geographically distributed in cropped fields in the northwest of this area, whereas melioidosis cases are distributed in a densely populated district in the southeast. We hypothesized that contaminated cropped fields generated aerosols contaminated with B. pseudomallei, which were carried by a northwesterly wind to the densely populated southeastern district. We collected soil and aerosol samples from a 72 km(2) area of land, including the melioidosis-clustered area and its surroundings. Aerosols that contained B. pseudomallei-specific TTSS (type III secretion system) ORF2 DNA were well distributed in the endemic area but were rare in the surrounding areas during the rainy season. The concentration of this specific DNA in aerosols was positively correlated with the incidence of melioidosis and the appearance of a northwesterly wind. Moreover, the isolation rate in the superficial layers of the contaminated cropped field in the northwest was correlated with PCR positivity for aerosols collected from the southeast over a 2-year period. According to pulsed-field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) analyses, PFGE Type Ia (ST58) was the predominant pattern linking the molecular association among soil, aerosol and human isolates. Thus, the airborne transmission of melioidosis moves from the contaminated soil to aerosols and/or to humans in this endemic area. Public Library of Science 2015-06-10 /pmc/articles/PMC4462588/ /pubmed/26061639 http://dx.doi.org/10.1371/journal.pntd.0003834 Text en © 2015 Chen et al http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited. |
spellingShingle | Research Article Chen, Pei-Shih Chen, Yao-Shen Lin, Hsi-Hsun Liu, Pei-Ju Ni, Wei-Fan Hsueh, Pei-Tan Liang, Shih-Hsiung Chen, Chialin Chen, Ya-Lei Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei |
title | Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei
|
title_full | Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei
|
title_fullStr | Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei
|
title_full_unstemmed | Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei
|
title_short | Airborne Transmission of Melioidosis to Humans from Environmental Aerosols Contaminated with B. pseudomallei
|
title_sort | airborne transmission of melioidosis to humans from environmental aerosols contaminated with b. pseudomallei |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462588/ https://www.ncbi.nlm.nih.gov/pubmed/26061639 http://dx.doi.org/10.1371/journal.pntd.0003834 |
work_keys_str_mv | AT chenpeishih airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT chenyaoshen airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT linhsihsun airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT liupeiju airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT niweifan airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT hsuehpeitan airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT liangshihhsiung airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT chenchialin airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei AT chenyalei airbornetransmissionofmelioidosistohumansfromenvironmentalaerosolscontaminatedwithbpseudomallei |