Cargando…

RapGene: a fast and accurate strategy for synthetic gene assembly in Escherichia coli

The ability to assemble DNA sequences de novo through efficient and powerful DNA fabrication methods is one of the foundational technologies of synthetic biology. Gene synthesis, in particular, has been considered the main driver for the emergence of this new scientific discipline. Here we describe...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zampini, Massimiliano, Stevens, Pauline Rees, Pachebat, Justin A., Kingston-Smith, Alison, Mur, Luis A. J., Hayes, Finbarr
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Nature Publishing Group 2015
Materias:	Article
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462754/ https://www.ncbi.nlm.nih.gov/pubmed/26062748 http://dx.doi.org/10.1038/srep11302

Ejemplares similares

Terminator Operon Reporter: combining a transcription termination switch with reporter technology for improved gene synthesis and synthetic biology applications
por: Zampini, Massimiliano, et al.
Publicado: (2016)

Combinatorial targeting of ribbon–helix–helix artificial transcription factors to chimeric recognition sites
por: Zampini, Massimiliano, et al.
Publicado: (2012)

Segrosome assembly at the pliable parH centromere
por: Wu, Meiyi, et al.
Publicado: (2011)

Comparative Metabolite Fingerprinting of the Rumen System during Colonisation of Three Forage Grass (Lolium perenne L.) Varieties
por: Kingston-Smith, Alison H., et al.
Publicado: (2013)

Design Parameters to Control Synthetic Gene Expression in Escherichia coli
por: Welch, Mark, et al.
Publicado: (2009)

Design and synthesis of synthetic UP elements for modulation of gene expression in Escherichia coli
por: Presnell, Kristin V., et al.
Publicado: (2019)

Engineered Orthogonal Quorum Sensing Systems for Synthetic Gene Regulation in Escherichia coli
por: Tekel, Stefan J., et al.
Publicado: (2019)

Design of simple synthetic RNA thermometers for temperature-controlled gene expression in Escherichia coli
por: Neupert, Juliane, et al.
Publicado: (2008)

Novel recA-Independent Horizontal Gene Transfer in Escherichia coli K-12
por: Kingston, Anthony W., et al.
Publicado: (2015)

A fast, robust, and tunable synthetic gene oscillator
por: Stricker, Jesse, et al.
Publicado: (2008)

A Bayesian Approach for Fast and Accurate Gene Tree Reconstruction
por: Rasmussen, Matthew D., et al.
Publicado: (2011)

Deep Learning Enables Fast and Accurate Imputation of Gene Expression
por: Viñas, Ramon, et al.
Publicado: (2021)

Correction: Novel recA-Independent Horizontal Gene Transfer in Escherichia coli K-12
por: Kingston, Anthony W., et al.
Publicado: (2015)

Accurate, Model-Based Tuning of Synthetic Gene Expression Using Introns in S. cerevisiae
por: Yofe, Ido, et al.
Publicado: (2014)

Designing artificial synthetic promoters for accurate, smart, and versatile gene expression in plants
por: Yasmeen, Erum, et al.
Publicado: (2023)

Engineering and application of synthetic nar promoter for fine-tuning the expression of metabolic pathway genes in Escherichia coli
por: Hwang, Hee Jin, et al.
Publicado: (2018)

Two New Plasmid Post-segregational Killing Mechanisms for the Implementation of Synthetic Gene Networks in Escherichia coli
por: Fedorec, Alex J.H., et al.
Publicado: (2019)

Small RNA‐binding protein RapZ mediates cell envelope precursor sensing and signaling in Escherichia coli
por: Khan, Muna A, et al.
Publicado: (2020)

Highly multiplexed, fast and accurate nanopore sequencing for verification of synthetic DNA constructs and sequence libraries
por: Currin, Andrew, et al.
Publicado: (2019)

NINJA-OPS: Fast Accurate Marker Gene Alignment Using Concatenated Ribosomes
por: Al-Ghalith, Gabriel A., et al.
Publicado: (2016)

Fast and accurate gene regulatory network inference by normalized least squares regression
por: Hillerton, Thomas, et al.
Publicado: (2022)

RapMap: a rapid, sensitive and accurate tool for mapping RNA-seq reads to transcriptomes
por: Srivastava, Avi, et al.
Publicado: (2016)

Microbiomes attached to fresh perennial ryegrass are temporally resilient and adapt to changing ecological niches
por: Huws, Sharon A., et al.
Publicado: (2021)

Correction to: Microbiomes attached to fresh perennial ryegrass are temporally resilient and adapt to changing ecological niches
por: Huws, Sharon A., et al.
Publicado: (2021)

In Vivo Transcription Kinetics of a Synthetic Gene Uninvolved in Stress-Response Pathways in Stressed Escherichia coli Cells
por: Muthukrishnan, Anantha-Barathi, et al.
Publicado: (2014)

Engineering Escherichia coli FAB system using synthetic plant genes for the production of long chain fatty acids
por: Kassab, Elias, et al.
Publicado: (2019)

Structural basis of RNA polymerase recycling by the Swi2/Snf2 family of ATPase RapA in Escherichia coli
por: Qayyum, M. Zuhaib, et al.
Publicado: (2021)

Accurate differentiation of Escherichia coli and Shigella serogroups: challenges and strategies
por: Devanga Ragupathi, N.K., et al.
Publicado: (2017)

FastMulRFS: fast and accurate species tree estimation under generic gene duplication and loss models
por: Molloy, Erin K, et al.
Publicado: (2020)

A synthetic Escherichia coli predator–prey ecosystem
por: Balagaddé, Frederick K, et al.
Publicado: (2008)

Engineering a synthetic pathway for maleate in Escherichia coli
por: Noda, Shuhei, et al.
Publicado: (2017)

Protocols for Implementing an Escherichia coli Based TX-TL Cell-Free Expression System for Synthetic Biology
por: Sun, Zachary Z., et al.
Publicado: (2013)

LFMM 2: Fast and Accurate Inference of Gene-Environment Associations in Genome-Wide Studies
por: Caye, Kevin, et al.
Publicado: (2019)

Fast and accurate inference of gene regulatory networks through robust precision matrix estimation
por: Passemiers, Antoine, et al.
Publicado: (2022)

PCycDB: a comprehensive and accurate database for fast analysis of phosphorus cycling genes
por: Zeng, Jiaxiong, et al.
Publicado: (2022)

COrona-rap
por: van Hogendorp, Sophie
Publicado: (2020)

Rapping Mania
Publicado: (1853)

Spiritual Rappings
Publicado: (1853)

Rapping Mania
Publicado: (1853)

Seven gene deletions in seven days: Fast generation of Escherichia coli strains tolerant to acetate and osmotic stress
por: Jensen, Sheila I., et al.
Publicado: (2015)

Cannot write session to /tmp/vufind_sessions/sess_onobfom0mkpcmkbhj2p0chbvf1