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Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments

To determine how chromosome segregation is coordinated with nuclear envelope formation (NEF), we examined the dynamics of NEF in the presence of lagging acentric chromosomes in Drosophila neuroblasts. Acentric chromosomes often exhibit delayed but ultimately successful segregation and incorporation...

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Detalles Bibliográficos
Autores principales: Karg, Travis, Warecki, Brandt, Sullivan, William
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The American Society for Cell Biology 2015
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462941/
https://www.ncbi.nlm.nih.gov/pubmed/25877868
http://dx.doi.org/10.1091/mbc.E15-01-0026
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author Karg, Travis
Warecki, Brandt
Sullivan, William
author_facet Karg, Travis
Warecki, Brandt
Sullivan, William
author_sort Karg, Travis
collection PubMed
description To determine how chromosome segregation is coordinated with nuclear envelope formation (NEF), we examined the dynamics of NEF in the presence of lagging acentric chromosomes in Drosophila neuroblasts. Acentric chromosomes often exhibit delayed but ultimately successful segregation and incorporation into daughter nuclei. However, it is unknown whether these late-segregating acentric fragments influence NEF to ensure their inclusion in daughter nuclei. Through live analysis, we show that acentric chromosomes induce highly localized delays in the reassembly of the nuclear envelope. These delays result in a gap in the nuclear envelope that facilitates the inclusion of lagging acentrics into telophase daughter nuclei. Localized delays of nuclear envelope reassembly require Aurora B kinase activity. In cells with reduced Aurora B activity, there is a decrease in the frequency of local nuclear envelope reassembly delays, resulting in an increase in the frequency of acentric-bearing, lamin-coated micronuclei. These studies reveal a novel role of Aurora B in maintaining genomic integrity by promoting the formation of a passageway in the nuclear envelope through which late-segregating acentric chromosomes enter the telophase daughter nucleus.
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spelling pubmed-44629412015-08-30 Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments Karg, Travis Warecki, Brandt Sullivan, William Mol Biol Cell Articles To determine how chromosome segregation is coordinated with nuclear envelope formation (NEF), we examined the dynamics of NEF in the presence of lagging acentric chromosomes in Drosophila neuroblasts. Acentric chromosomes often exhibit delayed but ultimately successful segregation and incorporation into daughter nuclei. However, it is unknown whether these late-segregating acentric fragments influence NEF to ensure their inclusion in daughter nuclei. Through live analysis, we show that acentric chromosomes induce highly localized delays in the reassembly of the nuclear envelope. These delays result in a gap in the nuclear envelope that facilitates the inclusion of lagging acentrics into telophase daughter nuclei. Localized delays of nuclear envelope reassembly require Aurora B kinase activity. In cells with reduced Aurora B activity, there is a decrease in the frequency of local nuclear envelope reassembly delays, resulting in an increase in the frequency of acentric-bearing, lamin-coated micronuclei. These studies reveal a novel role of Aurora B in maintaining genomic integrity by promoting the formation of a passageway in the nuclear envelope through which late-segregating acentric chromosomes enter the telophase daughter nucleus. The American Society for Cell Biology 2015-06-15 /pmc/articles/PMC4462941/ /pubmed/25877868 http://dx.doi.org/10.1091/mbc.E15-01-0026 Text en © 2015 Karg, Warecki, and Sullivan. This article is distributed by The American Society for Cell Biology under license from the author(s). Two months after publication it is available to the public under an Attribution–Noncommercial–Share Alike 3.0 Unported Creative Commons License (http://creativecommons.org/licenses/bync-sa/3.0). “ASCB®,” “The American Society for Cell Biology®,” and “Molecular Biology of the Cell®” are registered trademarks of The American Society for Cell Biology.
spellingShingle Articles
Karg, Travis
Warecki, Brandt
Sullivan, William
Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments
title Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments
title_full Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments
title_fullStr Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments
title_full_unstemmed Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments
title_short Aurora B–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments
title_sort aurora b–mediated localized delays in nuclear envelope formation facilitate inclusion of late-segregating chromosome fragments
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462941/
https://www.ncbi.nlm.nih.gov/pubmed/25877868
http://dx.doi.org/10.1091/mbc.E15-01-0026
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