Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions

[Image: see text] We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high nu...

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Detalles Bibliográficos
Autores principales: Shechtman, Yoav, Weiss, Lucien E., Backer, Adam S., Sahl, Steffen J., Moerner, W. E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2015
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462996/
https://www.ncbi.nlm.nih.gov/pubmed/25939423
http://dx.doi.org/10.1021/acs.nanolett.5b01396
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author Shechtman, Yoav
Weiss, Lucien E.
Backer, Adam S.
Sahl, Steffen J.
Moerner, W. E.
author_facet Shechtman, Yoav
Weiss, Lucien E.
Backer, Adam S.
Sahl, Steffen J.
Moerner, W. E.
author_sort Shechtman, Yoav
collection PubMed
description [Image: see text] We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells.
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spelling pubmed-44629962016-05-05 Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions Shechtman, Yoav Weiss, Lucien E. Backer, Adam S. Sahl, Steffen J. Moerner, W. E. Nano Lett [Image: see text] We employ a novel framework for information-optimal microscopy to design a family of point spread functions (PSFs), the Tetrapod PSFs, which enable high-precision localization of nanoscale emitters in three dimensions over customizable axial (z) ranges of up to 20 μm with a high numerical aperture objective lens. To illustrate, we perform flow profiling in a microfluidic channel and show scan-free tracking of single quantum-dot-labeled phospholipid molecules on the surface of living, thick mammalian cells. American Chemical Society 2015-05-05 2015-06-10 /pmc/articles/PMC4462996/ /pubmed/25939423 http://dx.doi.org/10.1021/acs.nanolett.5b01396 Text en Copyright © 2015 American Chemical Society This is an open access article published under an ACS AuthorChoice License (http://pubs.acs.org/page/policy/authorchoice_termsofuse.html) , which permits copying and redistribution of the article or any adaptations for non-commercial purposes.
spellingShingle Shechtman, Yoav
Weiss, Lucien E.
Backer, Adam S.
Sahl, Steffen J.
Moerner, W. E.
Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
title Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
title_full Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
title_fullStr Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
title_full_unstemmed Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
title_short Precise Three-Dimensional Scan-Free Multiple-Particle Tracking over Large Axial Ranges with Tetrapod Point Spread Functions
title_sort precise three-dimensional scan-free multiple-particle tracking over large axial ranges with tetrapod point spread functions
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4462996/
https://www.ncbi.nlm.nih.gov/pubmed/25939423
http://dx.doi.org/10.1021/acs.nanolett.5b01396
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