Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients

A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA conce...

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Autores principales: Bryzgunova, O. E., Tamkovich, S. N., Cherepanova, A. V., Yarmoshchuk, S. V., Permyakova, V. I., Anykeeva, O. Y., Laktionov, P. P.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: A.I. Gordeyev 2015
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463421/
https://www.ncbi.nlm.nih.gov/pubmed/26085953
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author Bryzgunova, O. E.
Tamkovich, S. N.
Cherepanova, A. V.
Yarmoshchuk, S. V.
Permyakova, V. I.
Anykeeva, O. Y.
Laktionov, P. P.
author_facet Bryzgunova, O. E.
Tamkovich, S. N.
Cherepanova, A. V.
Yarmoshchuk, S. V.
Permyakova, V. I.
Anykeeva, O. Y.
Laktionov, P. P.
author_sort Bryzgunova, O. E.
collection PubMed
description A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface. The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total pool of circulating DNA (cfDNA + csbDNA) should be used for a correct analysis of marker DNA concentrations and data standardization.
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spelling pubmed-44634212015-06-17 Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients Bryzgunova, O. E. Tamkovich, S. N. Cherepanova, A. V. Yarmoshchuk, S. V. Permyakova, V. I. Anykeeva, O. Y. Laktionov, P. P. Acta Naturae Research Article A direct correlation between the concentration of cell-free and cell-surface-bound circulating DNA (cfDNA and csbDNA, respectively) was demonstrated. Based on an inverse correlation between blood plasma DNase activity and the cfDNA concentration, blood DNases are supposed to regulate the cfDNA concentration. However, no correlation was found between the DNase activity in blood plasma and the csbDNA concentration, indicating that blood DNases are not involved in csbDNA dissociation from the cell surface. The possibility of DNA redistribution between cfDNA and csbDNA indicates that the total pool of circulating DNA (cfDNA + csbDNA) should be used for a correct analysis of marker DNA concentrations and data standardization. A.I. Gordeyev 2015 /pmc/articles/PMC4463421/ /pubmed/26085953 Text en Copyright ® 2015 Park-media Ltd. http://creativecommons.org/licenses/by/2.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Bryzgunova, O. E.
Tamkovich, S. N.
Cherepanova, A. V.
Yarmoshchuk, S. V.
Permyakova, V. I.
Anykeeva, O. Y.
Laktionov, P. P.
Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients
title Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients
title_full Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients
title_fullStr Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients
title_full_unstemmed Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients
title_short Redistribution of Free- and Cell-Surface-Bound DNA in Blood of Benign and Malignant Prostate Tumor Patients
title_sort redistribution of free- and cell-surface-bound dna in blood of benign and malignant prostate tumor patients
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4463421/
https://www.ncbi.nlm.nih.gov/pubmed/26085953
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